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DrMushroom
Human Farmer


Registered: 04/28/12
Posts: 722
Loc: Australia
Last seen: 23 days, 7 hours
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Can i put an air purifier in my still air box and if so what kind
#23382048 - 06/25/16 08:19 PM (7 years, 7 months ago) |
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I tried pretty hard but i still wound up getting a whole bunch of contaminated petri dishes, and likely even contams from the air too in jars.
I live in rural australia so the strongest disinfectant spray i can get is glen 20.
I was wondering, if there are any practical sorts of little 12v/usb air ionizers or purifiers of sorts i can run in the SAB for the day before i do anything.
I have a nebulizer which would fog the whole thing up which could make all the dust/mold settle, however im not sure about how things work when everything goes wet, like, is it neutral/bad, increase contams/etc?
I worry about sprays since i am doing the flame sterilization.
Another idea i had was to find a small cheap HEPA filter and use that to clean the air, something like that would warrant ramping up the box lid seals/sacrifice ease of opening. Of course, i wouldnt run it while im doing anything, this is just to prep the air.
Otherwise im stumped, i clean the box with any combination of iso, peroxide and bleach, plus ordinary methylated spirits afterwards for cleaning the glass up, the box uses disposeable sleeves and gloves that seal well and i replace each time though i still wash it all with iso before i do anything. Last time i also ran a sterilizing UV light over everything to kill off anything in the air with that light but, no good. There were less contams than normal, but still contams all the same. The only thing i dont do is work in a wet box, because water gets everywhere and filthy.
Also could anyone post links to a video showing someone else doing agar work/etc id like to see how someone else works, albeit in a glove box.
Edited by DrMushroom (06/25/16 08:21 PM)
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FriedEgg


Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Re: Can i put an air purifier in my still air box and if so what kind [Re: DrMushroom]
#23384766 - 06/26/16 05:23 PM (7 years, 7 months ago) |
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no. SAB stands for "Still air box", not "Sterile Air Box". even if you could filter the air inside, it would be pointless because you have to open it and stick your hands in there.
here's what everyone else does and it works fine: fill up a spray bottle with plain water + a few drops of dish soap. you don't need bleach or alcohol. spray the shit out of the walls, floor, and ceiling of your SAB with your soap/water mixture. load up your SAB with your jars and anything else. spray some more from high above, causing a fine mist. close the lid and wait 2-5 minutes. then stick your hands in the holes and get to work! if that doesn't work, it's your poor sterile tek that is causing contams, not the SAB itself. if you still need some help, search for "sterile tek" and practice it.
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DrMushroom
Human Farmer


Registered: 04/28/12
Posts: 722
Loc: Australia
Last seen: 23 days, 7 hours
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Re: Can i put an air purifier in my still air box and if so what kind [Re: FriedEgg]
#23385759 - 06/26/16 09:26 PM (7 years, 7 months ago) |
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actually because of the disposeable sleeves i dont have to open the box when i put my arms in. They are attached to the box. But if im spraying atuff in and around then i cant use the filter.
Just to clarify, wet is good though, or ok?
I thought it would make the contam risk worse, and everything would get filthy
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     Mushroom Madness for my face and the tree on the moon dinosaur hello yellow im game. Shaman Australis Botanicals and Mycology (ethnobotany and mycology, an australia based discord) https://discord.gg/UYaH6QT
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FriedEgg


Registered: 09/22/14
Posts: 2,536
Loc: Taiwan
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Re: Can i put an air purifier in my still air box and if so what kind [Re: DrMushroom]
#23386386 - 06/27/16 02:15 AM (7 years, 7 months ago) |
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you should get rid of the gloves and just cut arm holes. a "glove box" like you are describing can cause suction and wild air movements that you don't get with a SAB. yes, wet is fine. just let the air settle for a minute or two after you close the lid.
for sterile tek, make sure you don't put your hand over anything. just search youtube for "aseptic technique", "sterile tek", and "sterile technique". there are lots of great vidoes you can learn from. even the nursing and surgery videos have good info too.
-------------------- (Yes, the egg is real)
How to post pics
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DrMushroom
Human Farmer


Registered: 04/28/12
Posts: 722
Loc: Australia
Last seen: 23 days, 7 hours
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Re: Can i put an air purifier in my still air box and if so what kind [Re: FriedEgg]
#23386646 - 06/27/16 05:44 AM (7 years, 7 months ago) |
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again, no. the gloves go on my hands, it has medical sterile sleeves that i attach to cover my arms, i did previously have a fair bit of success with a long gloved box that was sealed and i also noticed that issue though, it wasnt too bad, its almost non-existent here. still, no reason i cant just not stick the sleeve over the arm hole.
Thats good to know though, i just had an idea. to reduce contams on my dishes i might try building a rack or stand so the dishes are quite high up. the top of the box is glass and well cleaned/sealed so it shouldnt be any more dangerous up above than below.
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bigbigbilly
Stranger
Registered: 07/05/12
Posts: 127
Last seen: 2 months, 5 days
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Re: Can i put an air purifier in my still air box and if so what kind [Re: DrMushroom]
#23396303 - 06/30/16 04:01 AM (7 years, 6 months ago) |
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DrMushroom
Human Farmer


Registered: 04/28/12
Posts: 722
Loc: Australia
Last seen: 23 days, 7 hours
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Re: Can i put an air purifier in my still air box and if so what kind [Re: bigbigbilly]
#23396582 - 06/30/16 06:59 AM (7 years, 6 months ago) |
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that looks interesting though its not what im after. I just wanted to lessen available contaminants in the still air box, since once sealed non more would enter. That way theres less contams available to become airborne. I dont want to make a positive pressure box, though that certainly does look cool, must be tricky though flame sterilizing stuff in there unless your using an induction coil.
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