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OfflineBobhopeing
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Registered: 05/04/16
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Last seen: 7 years, 7 months
Pasteurization question?
    #23381668 - 06/25/16 06:13 PM (7 years, 7 months ago)

ok, so this is my 3rd summer doing this and i do the bucket tek. i have a 70 to 80 percent susses rate with monotubs. and this summer i already lost 6 out of 10 tubs and im fed up, im assuming its pasturizing problems because i know its not my jars. does propery pasturizing really make a 100% success rate and does it really improve everything.  i would like to hear what a  bunch of other people think.

and i just clean my tubs with soap and bleach, that should bo ok right?
or should i wipe with isprol al.


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Invisibledankington
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Re: Pasteurization question? [Re: Bobhopeing]
    #23381702 - 06/25/16 06:24 PM (7 years, 7 months ago)

It's your spawn. I bet.


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OfflineBobhopeing
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Re: Pasteurization question? [Re: dankington]
    #23381710 - 06/25/16 06:27 PM (7 years, 7 months ago)

well that sux,lol,,,i didnt thinks so because this hasent happened till this year.i have awesoem resultes on 8 or 9 out 10 tubs in the past 2 years and this year has just thrown me for a loop.


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Invisibledankington
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Re: Pasteurization question? [Re: Bobhopeing]
    #23381714 - 06/25/16 06:28 PM (7 years, 7 months ago)

yeah I mean. It's like this: you can get away without wiping after you shit once in a while, but eventually you'll stain your underpants. Did you do spore syringe to grains?


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OfflineMycologist217
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Re: Pasteurization question? [Re: dankington]
    #23381738 - 06/25/16 06:33 PM (7 years, 7 months ago)

What kind of substrate are you using? Coir? If so I would also be inclined to believe your spawn is the issue.

If your substrate is properly pasteurized and your spawn is clean you WILL NOT see a contamination before your first flush.....

It's hard to believe that the bucket tek would fail to properly pasteurize coir 6/10 times...which is why I also think you are working with contaminated spawn.


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Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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OfflineBobhopeing
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Re: Pasteurization question? [Re: Mycologist217]
    #23381757 - 06/25/16 06:40 PM (7 years, 7 months ago)

ok, i get what you guys are saying. the grains were just done the way i always done and the looked healthy white myc grew quick


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Invisibledankington
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Re: Pasteurization question? [Re: Bobhopeing]
    #23381764 - 06/25/16 06:42 PM (7 years, 7 months ago)

not necessarily good though. There're visible signs to look for, fast isn't always good.

Did you do spore syringe to grain??


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OfflineBobhopeing
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Re: Pasteurization question? [Re: dankington]
    #23381794 - 06/25/16 06:49 PM (7 years, 7 months ago)

yup, GT to wbs


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Invisibledankington
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Re: Pasteurization question? [Re: Bobhopeing]
    #23381799 - 06/25/16 06:50 PM (7 years, 7 months ago)

that's the problem. Spores aren't clean, friend. So you're gambling going about it that way.


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OfflineBobhopeing
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Re: Pasteurization question? [Re: dankington]
    #23381802 - 06/25/16 06:51 PM (7 years, 7 months ago)

got them from the depot, they came dirty then


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Invisibledankington
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Re: Pasteurization question? [Re: Bobhopeing]
    #23381804 - 06/25/16 06:52 PM (7 years, 7 months ago)

spores typically are. don't blame the vendor, please.
When you grow mushrooms in a 100% sterile environment, then you can judge.


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OfflineBobhopeing
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Re: Pasteurization question? [Re: dankington]
    #23381812 - 06/25/16 06:55 PM (7 years, 7 months ago)

well, im pretty darn clean when in do my wbs and when i inject jars...wasent blaming vendor, more of a question. dint mean to offend bro.


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Invisibledankington
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Re: Pasteurization question? [Re: Bobhopeing]
    #23381816 - 06/25/16 06:56 PM (7 years, 7 months ago)

no it's just that spores aren't clean. Try pastywhyte's easy agar tek to clean your growth up :thumbup:

Or do BRF cakes.


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OfflineBobhopeing
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Re: Pasteurization question? [Re: dankington]
    #23381818 - 06/25/16 06:57 PM (7 years, 7 months ago)

well im on to more so no worries, just wondering if it was pasturizing, well if its my wbs that sucks, is rye grass seed better to deal with,


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OfflineMycologist217
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Re: Pasteurization question? [Re: Bobhopeing]
    #23381827 - 06/25/16 06:59 PM (7 years, 7 months ago)

You are not understanding what he is saying....no matter if you use rye or birdseed, or whatever...,,if you continue growing mushrooms with spores instead of clean mycelium produced with agar work, you will continue see contaminations....,spores come from mushrooms that are growing in open air...,,thus all spores are dirty....and all spore based grows will run the risk of perpetuating the filth into a grow ruining contamination....


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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OfflineLeftfield420
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Re: Pasteurization question? [Re: Bobhopeing]
    #23381885 - 06/25/16 07:17 PM (7 years, 7 months ago)

No spore syringe is 100% clean....ever


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InvisibleSnazz
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Re: Pasteurization question? [Re: Leftfield420]
    #23382343 - 06/25/16 09:50 PM (7 years, 7 months ago)

Paddington Bear is 100% correct.  It's always a crap-shoot from syringe unless you start with petri dishes/pasty plates


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InvisibleCosmoKramer
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Registered: 06/22/16
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Re: Pasteurization question? [Re: Snazz]
    #23382357 - 06/25/16 09:54 PM (7 years, 7 months ago)

There's a lot of contam vectors with syringes even if you make them yourself. Best to always get a couple of clean transfers first so your not wasting time, money, and headaches. Save all of that for real life problems !


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OfflineHybridprX
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Re: Pasteurization question? [Re: CosmoKramer]
    #23383054 - 06/26/16 05:55 AM (7 years, 7 months ago)

Properly prepared spore prints are not dirty...

I can go straight from foil to syringe with 98% of the prints that I've received threw trade and my own.... if they're mishandled during use its the cultivator that interduces the contamination when making the spore syringe.

Always use a test jar if you don't have the means to use agar.

The benefit to agar is the ability to isolate a clean culture "if" the print/syringe is dirty.


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Invisiblemupetmower
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Re: Pasteurization question? [Re: HybridprX]
    #23383063 - 06/26/16 06:07 AM (7 years, 7 months ago)

unless the print came from a fruit that was grown in sterile conditions, then its not going to be 100% clean. no matter how well your sterile technique is. and lets face it, i doubt many prints are taken in 100% sterile conditions. especially when youre a vendor who is having to put out tons of fruits to make all the prints. its just not happening.


that doesnt mean you cant use a syringe from a vendor. you just gotta clean it up on agar first if you want to ensure it' clean before inocing grain.


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-The wise man never stops seeking knowledge.


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