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Invisiblemarkisses
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Registered: 09/17/06
Posts: 68
Spore solution on Agar
    #23380787 - 06/25/16 01:00 PM (7 years, 7 months ago)

I have five agar plates and some multi-spore solution.

I was originally going to place one drop of solution in
the middle of one plate, wait until it colonized, and
transfer each sector to its own quart of grain. Then
repeat this process with the other plates.

Now, I'm second guessing the original plan because
I have done more research on agar work.

Can you all give me some suggestions given my situation?


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Invisiblemupetmower
Mower of Muppets
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Registered: 03/29/16
Posts: 3,036
Loc: Here and There
Re: Spore solution on Agar [Re: markisses]
    #23380859 - 06/25/16 01:27 PM (7 years, 7 months ago)

get more plates. make a few with a drop of solution. transfer from those plates to new plates. do this as many times as it takes until you have a clean culture. general rule of thumb is that even if it looks clean, a plate made from spore syringe or print should be given at least 1 transfer to ensure it is clean, before putting it to grain. but honestly, you might need to do more than just 1 transfer to ensure a clean culture, considering prints and spore syringes are rarely clean.

Pasty Plates


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


Edited by mupetmower (06/25/16 01:28 PM)


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Invisiblefilthyknees
no coincidence
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Registered: 03/08/13
Posts: 6,283
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Re: Spore solution on Agar [Re: mupetmower]
    #23381035 - 06/25/16 02:43 PM (7 years, 7 months ago)

when your plates germinate & grow to about the size of a dime make the transfers of only the best growth to new plates.

After those (1st transfer)grow out to dime size (about a week later) I'd do another set of transfers(2nd transfer) new plates (how ever many agar grain master jars you want to end up expanding(plus a few)).

using one plate --> one jar will reduce the risk of contamination, along with a few quick transfers from healthy growth ..... :mushroom2:


--------------------
But if you're in a hurry, and really got to go
If you're in a hurry, might have to find out slow
That it's one thing to try and another to fly
You get there quicker just a step at a time
It's one thing to bark, another to bite
The show ain't over till you pack up at night


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OfflinePsykronik
OverGrower
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Registered: 02/28/12
Posts: 150
Loc: Scotland Flag
Last seen: 7 years, 4 months
Re: Spore solution on Agar [Re: filthyknees]
    #23381401 - 06/25/16 04:39 PM (7 years, 7 months ago)

What I have done is :
prepared 9 PDA plates.
Inoculated 3 with 3 separate strains of cubes ( just a drop of MS syringe ).
The 3 plates are now colonising.
I will isolate and transfer a wedge from each plate onto another plate. So I will have 6 plates. ( 3 plates of master and 3 plates of a transfer )
I will store the master in the fridge for future.
Wait until the 3 transfer plates colonise and then repeat the isolation and transferring steps.
I will then put the 1st transferred plates into fridge.
I will be left with 3 plates that have been transferred twice so the chance of a clean culture to work with is at its highest.


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