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ColonelAngus
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Does gravity affect mycelium growth in colonizing jars? (Pic)
#23380153 - 06/25/16 09:35 AM (7 years, 7 months ago) |
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I've got some 2qt jars of rye, and one is slightly stalled in just one strip. I was thinking maybe if I lay the jar on it's side with the stalled part down, maybe gravity would pull the growth in that direction?
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CosmoKramer
The Assman


Registered: 06/22/16
Posts: 555
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Re: Does gravity affect mycelium growth in colonizing jars? (Pic) [Re: ColonelAngus]
#23380210 - 06/25/16 09:53 AM (7 years, 7 months ago) |
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Mycelium in the colonization stage doesn't really respond to gravity changes that I'm aware of. However if the grains being dry in that one stripe is what stalled the colonization, gravity could perhaps pull moisture or sweat down from the colonized areas. Not sure but it's a just an idea. However I've spawned stalled grains at 90% with no problems, but it has its risks of contaminating your substrate. If it wasn't in the middle you could scrape or try to hold back as much of the stalled grains as possible.
How long has it been colonizing and did you shake around 20-30%?
-------------------- "Get yourself some vitamin C with rose hips and bioflavonoids."
Edited by CosmoKramer (06/25/16 09:55 AM)
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TheChief
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Re: Does gravity affect mycelium growth in colonizing jars? (Pic) [Re: ColonelAngus]
#23380250 - 06/25/16 10:03 AM (7 years, 7 months ago) |
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This may answer your question.

If your jar has stalled it likely has a problem. The only thing I can suggest is give it a shake to see if it recovers and how it smells. Even then you still may be running the risk of using a contaminated or weak culture. The best bet is just to toss questionable jars.
Edited by TheChief (06/25/16 06:03 PM)
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CosmoKramer
The Assman


Registered: 06/22/16
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Re: Does gravity affect mycelium growth in colonizing jars? (Pic) [Re: TheChief]
#23380360 - 06/25/16 10:37 AM (7 years, 7 months ago) |
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Quote:
TheChief said: This may answer your question.

If your jar is stalled it likely has a problem. The only thing I can suggest is give it a shake to see if it recovers and how it smells. Even then you still may be running the risk of using a contaminated or weak culture. The best bet is just to toss questionable jars.

-------------------- "Get yourself some vitamin C with rose hips and bioflavonoids."
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ColonelAngus
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Re: Does gravity affect mycelium growth in colonizing jars? (Pic) [Re: ColonelAngus]
#23380384 - 06/25/16 10:43 AM (7 years, 7 months ago) |
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I shook it once, and it's not officially stalled yet. I did G2G with about a pint into the 1/2 gallon jar on 6/13, so 12 days ago.
I'm just being impatient probably, but the grains do look a tad dry on that side, so I'll try the lay-down and see if it picks up the pace.
Also, if the grains are a bit dry, I read a lot about dunking the grains before spawning and think I'll try it.
I saw most guys saying they shake before putting water in, but that sounds like you'd wash away a lot of mycelium.
Anyone have an opinion on just putting some water in the jar before shaking, then drain it off and shake then spawn?
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GreenRabbit
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Re: Does gravity affect mycelium growth in colonizing jars? (Pic) [Re: ColonelAngus]
#23380408 - 06/25/16 10:50 AM (7 years, 7 months ago) |
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Quote:
CosmoKramer said: Mycelium in the colonization stage doesn't really respond to gravity changes that I'm aware of.
I doubt this is completely true. It is hard to know exactly how the mycelium grows. It shouldn't matter, since mycelium grows in all directions, but we have limited knowledge as to how fungi respond to low gravity.
Quote:
zeronio said: I found an interesting article about gravity and mushrooms. The funny thing is that mushrooms were already grown in space. 
This experiment is showing gravitropic orientation of Flammulina velutipes fruiting bodies under different gravitational conditions. This picture show fruiting bodies grown for 5 days on Earth:
 as a control for fruiting bodies grown in orbit for 7 days during Spacelab D-2 mission:

Note the regular vertical orientation of the control culture and the random orientation of the fruiting bodies produced under conditions of microgravity.
Source: http://www.mssf.org/mnews/0303mn.pdf
Quote:
ColonelAngus said: maybe gravity would pull the growth in that direction?
This is definitely not how it works though. Look at the grain at the top of your jar. I bet there is some mycelium growing straight up. Did you ever shake this jar?
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CosmoKramer
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Re: Does gravity affect mycelium growth in colonizing jars? (Pic) [Re: GreenRabbit]
#23380427 - 06/25/16 10:57 AM (7 years, 7 months ago) |
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Was that experiment done only at the fruiting stage? Obviously that would result in a different outcome due to the weight of fruit bodies. As you said though, I don't believe gravity changes the direction of mycelium while colonizing. It goes wherever and however it wants.
-------------------- "Get yourself some vitamin C with rose hips and bioflavonoids."
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GreenRabbit
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Re: Does gravity affect mycelium growth in colonizing jars? (Pic) [Re: CosmoKramer]
#23380453 - 06/25/16 11:07 AM (7 years, 7 months ago) |
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Quote:
CosmoKramer said: Was that experiment done only at the fruiting stage? Obviously that would result in a different outcome due to the weight of fruit bodies.
I don't know. Possibly. The weight of the fruits doesn't explain it.. If that were the case, the ones grown on Earth would be droopy from gravity while the ones in space could grow upward due to weightlessness. They grow outward in space due to a lack of signalling. They don't know which way up is so they just grow straight out. I bet they would grow upwards and downwards as well if they were floating around.
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CosmoKramer
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Registered: 06/22/16
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Re: Does gravity affect mycelium growth in colonizing jars? (Pic) [Re: GreenRabbit]
#23380474 - 06/25/16 11:16 AM (7 years, 7 months ago) |
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Interesting! Anybody have a spaceship that me and greenrabbit can borrow? We have some experimenting to do.
-------------------- "Get yourself some vitamin C with rose hips and bioflavonoids."
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TheChief
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Re: Does gravity affect mycelium growth in colonizing jars? (Pic) [Re: GreenRabbit]
#23380489 - 06/25/16 11:21 AM (7 years, 7 months ago) |
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The mushrooms themselves definitely seem to know which way is up when gravity is involved. The myc itself probably does too but I doubt it plays much of a factor in how it colonizes a said substrate, as you can see it trying to colonize bare glass in the picture I posted. The mushrooms themselves have many reasons for growing upward, up is always where they find the best conditions to fruit. At the surface they find light, fresh air and moisture; all of these things are required to produce optimum fruit bodies.
My best opinion is to get rid of the jar, as it may cause you some headaches keeping it around. Take it from me, 1 jar of spawn is not worth the risk of spreading contamination around your grow area. Always empty or completely dispose of suspect jars outdoors.
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jcbowling1985 said: "Additionally I did one Armageddon jar with all of the types together."
Edited by TheChief (06/25/16 01:23 PM)
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spacechildo
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Re: Does gravity affect mycelium growth in colonizing jars? (Pic) [Re: ColonelAngus]
#23380509 - 06/25/16 11:30 AM (7 years, 7 months ago) |
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the mushrooms grown in space was an experiment on tropisms, cron has a great read on it in his journal.
Quote:
ColonelAngus said: I shook it once, and it's not officially stalled yet. I did G2G with about a pint into the 1/2 gallon jar on 6/13, so 12 days ago.
a pt of colonized grains should have colonized those 2 qts in no time, something's definitely fishy.
do a new shake and see what happens.
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ColonelAngus
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Re: Does gravity affect mycelium growth in colonizing jars? (Pic) [Re: ColonelAngus]
#23381490 - 06/25/16 05:11 PM (7 years, 7 months ago) |
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Well, not sure if it's the gravity or moisture, but the jar has made more progress today in that spot (after laying down on that side) than it has in 5 days.
Take a look:
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TheChief
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Re: Does gravity affect mycelium growth in colonizing jars? (Pic) [Re: ColonelAngus]
#23381506 - 06/25/16 05:18 PM (7 years, 7 months ago) |
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I'm not going to lie. Visually, this jar looks OK to me, albeit it looking a bit bacterial and needing a few more days. The description given is what makes me suspect otherwise.
Like I said, TOSS questionable jars.
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jcbowling1985 said: "Additionally I did one Armageddon jar with all of the types together."
Edited by TheChief (06/25/16 05:22 PM)
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ColonelAngus
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Re: Does gravity affect mycelium growth in colonizing jars? (Pic) [Re: TheChief]
#23381539 - 06/25/16 05:32 PM (7 years, 7 months ago) |
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Pray tell.. what part of it looks like bacteria? I did have a couple others go bad on me, and they started out really nice but never got this far along, and from what I'm looking at with this jar all the way around, it's looking solid and healthy.. but I definitely want to know what you're seeing.
Thx
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TheChief
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Re: Does gravity affect mycelium growth in colonizing jars? (Pic) [Re: ColonelAngus]
#23381620 - 06/25/16 05:57 PM (7 years, 7 months ago) |
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Well...
#1. The jar has stalled out on you before.
#2. Some of the grains have yellowing around them.
#3. The myc also does look a little suspect. Sometimes other organisms can hide and grow right beside cubes, or even look exactly like them, it is hard to tell if you've never seen it before.
#4. Your grains look overly wet.
#5. Quote:
spacechildo said:a pt of colonized grains should have colonized those 2 qts in no time, something's definitely fishy.
The first two of these reasons alone are grounds to dispose of the jar. They suggest that bacteria is involved, at the very least, if not something more sinister.
You can fruit bacterial jars with some success, just don't expect the best results. You should also be cautious if you decide to fruit them, because contams can arise at any time. It is best to toss bad jars, correct your mistakes, and start with a fresh culture off of agar that you are certain is clean.
Do not G2G jars that look as if they may be bacterial.
Also, I can see gravity playing a little role, if it were maybe used in a sense to manually move mycelium and moisture downward to promote growth in an uncolonized, dry area of substrate. Naturally, I think it grows where it finds nutrients and continues until it can't find anymore, this has nothing to do with gravity. At that point, it fruits to try and propagate spores in an attempt to continue the cycle.
Edited by TheChief (06/25/16 06:46 PM)
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ColonelAngus
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Re: Does gravity affect mycelium growth in colonizing jars? (Pic) [Re: TheChief]
#23381838 - 06/25/16 07:03 PM (7 years, 7 months ago) |
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Ok, thanks Chief! Very informative reply, so much appreciated. I'm starting to wonder if I'm making a mistake using these big 1/2 gallon jars. I just liked the idea of '1 jar / 1 tub' , but I'm thinking maybe the long colonization time is causing more of the grain to sweat, 'pee' or whatever, and allowing more time for nasties to take hold in what's otherwise nice healthy mycelium.
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