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Throwaway92214
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Registered: 03/30/16
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Last seen: 4 years, 10 months
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PCing slant questions
#23377830 - 06/24/16 03:15 PM (7 years, 7 months ago) |
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So I just put the agar into my tube however the cap to the tube doesn't create an air tight seal around the tube and as a result water can escape or get in if angled incorrectly. Should I put foil around the caps to prevent steam and condensation getting inside and messing up my volume measurements?
Secondly, I am using cellulose filter discs and I have read if they get wet then they can easily become contaminated and become a vector for infection. All of the slant TEKs require you to put water into a jar with a filter disc, will the steam coming from the inside get the bottom side of my disc wet and become a contamination vector?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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You will only be putting the slants in jars for sterilization. That's only to make the temps drop more slowly during de-pressurization so the agar won't boil over and exit the slant. You don't angle the slants in the PC, actually, it's better to leave them straight in there so the boiling won't cause a boil over. I don't use filter discs for PCing, I just leave my jar lids loose, just enough to prevent pressure from building.
Your slants shouldnt be completely sealed either, the pressure can make them crack. You will be wrapping them in parafilm after inoculation so you can have GE. It won't matter that they won't seal completely because you never really need to seal them unless they are unused and in storage. You could wrap them in parafilm during that time to protect them from contamination instead.
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Throwaway92214
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Quote:
Supalemonhaze said: You will only be putting the slants in jars for sterilization. That's only to make the temps drop more slowly during de-pressurization so the agar won't boil over and exit the slant. You don't angle the slants in the PC, actually, it's better to leave them straight in there so the boiling won't cause a boil over. I don't use filter discs for PCing, I just leave my jar lids loose, just enough to prevent pressure from building.
Your slants shouldnt be completely sealed either, the pressure can make them crack. You will be wrapping them in parafilm after inoculation so you can have GE. It won't matter that they won't seal completely because you never really need to seal them unless they are unused and in storage. You could wrap them in parafilm during that time to protect them from contamination instead.
I decided just to use the jars with the discs on them because there was condensation inside already, I'll just replace the SFD on top I guess. Unfortunately the slants are angled a little because there wasn't any room for an extra tube and I could only fit 4 inside my quart jar but it's not an extreme angle, if anything is ruined I can just start over tonight I guess.
Should I switch from cellulose to a SFD instead? I read that a SFD is okay to get wet because it's synthetic but cellulose is not. I'm debating on switching over, what do you think?
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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As I already explained, there is zero chance of contamination occuring due to a wet SFD.
1. The slants will be closed so even if you had some super powered mold spores in your house that could survive the PC's temp, they will just fall on the slant's caps.*edit: they also wouldn't have the time to germinate and grow through your SFD during the PC cycle, given that you have a supermold that is able to survive the extreme heat that is* 2. Contams get through wet filters during colonization and you will not be leaving the slants in the jars after PCing. What you will do, is take them out while still hot, and lay them on their side angled so they set at the desired angle. 3. Contams that grow through cellulose SFDs only do so when the filter is wet for a long time, allowing mold spores to germinate, since they can eat cellulose. They will then sporulate and those spores will drop on your grains. But of course, you don't have grains, you have slants and they will not be in there that long anyway.
Use whatever you have, as I also already mentioned, I don't use filters of any kind in my jars for slants. I just leave the lids loose so the pressure can escape. Slants are even easier to make than no-pour agar IMO, it's really hard to fuck them up. They don't have to be completely straight during Pcing either, it is just what is optimal.
Edited by Supalemonhaze (06/24/16 04:57 PM)
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Throwaway92214
Stranger

Registered: 03/30/16
Posts: 48
Last seen: 4 years, 10 months
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Quote:
Supalemonhaze said: As I already explained, there is zero chance of contamination occuring due to a wet SFD.
1. The slants will be closed so even if you had some super powered mold spores in your house that could survive the PC's temp, they will just fall on the slant's caps.*edit: they also wouldn't have the time to germinate and grow through your SFD during the PC cycle, given that you have a supermold that is able to survive the extreme heat that is* 2. Contams get through wet filters during colonization and you will not be leaving the slants in the jars after PCing. What you will do, is take them out while still hot, and lay them on their side angled so they set at the desired angle. 3. Contams that grow through cellulose SFDs only do so when the filter is wet for a long time, allowing mold spores to germinate, since they can eat cellulose. They will then sporulate and those spores will drop on your grains. But of course, you don't have grains, you have slants and they will not be in there that long anyway.
Use whatever you have, as I also already mentioned, I don't use filters of any kind in my jars for slants. I just leave the lids loose so the pressure can escape. Slants are even easier to make than no-pour agar IMO, it's really hard to fuck them up. They don't have to be completely straight during Pcing either, it is just what is optimal.
My main problem with the SFD thing being wet was because I planned on reusing the jar for grains in the future. I guess if I let them sit in my room and dry they'll be okay.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Correct. You could always skip the filter, just leave the lid loose and once the cycle finishes and the PC loses pressure, transfer to your already prepped SAB. Once you can handle the jar without scalding your hand, open it and lay them at an angle to set. The filter is meant to be a fail-safe but slants are too easy to mess up IMO. I've had more trouble with agar leaking than contamination in slants. My presto loses pressure very fast, boil overs with slants are not uncommon.
Remember to leave the slants slightly unscrewed or the air cause a vacuum during cooling, sucking in air the second you open them. Once cool, tighten the caps and if they don't seal properly, wrap in parafilm as well.
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