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DeadPhan



Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Anyone wipe the top of a master grain jar with iso before a g2g?
#23376951 - 06/24/16 10:26 AM (7 years, 7 months ago) |
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I work in front of a flowhood. I get good success rates when I'm working in smaller draft free enclosures. Pretty much 100 if not fault of iffy spawn. But sometimes I get a bad run. I sometimes look at my master jars and wonder if before g2ging I should give the rim a Lil once around with paper towel and alcohol. Or you think that's asking for more problems. Is everything under a lid and ring sterilized as the same as the whole jar in where it's unnecessary?
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
Edited by DeadPhan (06/24/16 10:32 AM)
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Anyone wipe the top of a master grain jar with is before a g2g? [Re: DeadPhan]
#23376972 - 06/24/16 10:31 AM (7 years, 7 months ago) |
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I wipe EVERYTHING with iso thorougly. Not rubbing or anything, just enough to get everywhere just a bit wet with iso. Then I take my iso bottle and squirt some under the lid before opening it. I don't always do that, just when I remember. Could be overkill, but it surely can't hurt.
If you are talking about the recieving jars that just came out of the PC, you can skip the wiping but if you are talking about the donor jars, it's better to wipe with iso.
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DeadPhan



Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
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Re: Anyone wipe the top of a master grain jar with is before a g2g? [Re: Supalemonhaze]
#23376990 - 06/24/16 10:34 AM (7 years, 7 months ago) |
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Talking about the rim of the master jar after I take the lid off.
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Anyone wipe the top of a master grain jar with is before a g2g? [Re: DeadPhan]
#23377008 - 06/24/16 10:38 AM (7 years, 7 months ago) |
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Oh, nah. Too risky IMO. Paper towel is likely to have contams and especially when working in front of a hood, they can easily end up inside. You should be able to get good enough results without it, I like to take every precaution available without having the risk of fucking it all up in the process. You are better off improving your techinique than relying on something as risky as that. It is very possible to get a 100% success rate over multiple batches without it.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
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Re: Anyone wipe the top of a master grain jar with is before a g2g? [Re: Supalemonhaze]
#23377100 - 06/24/16 11:01 AM (7 years, 7 months ago) |
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Maybe try this if you have 2 piece lids. Take the band off but leave the lid, wipe the "neck" where the band was covering. Then proceed to take off the lid and g2g
Under the ring isn't sterile unless it's right out of the pc
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 16 hours
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Re: Anyone wipe the top of a master grain jar with is before a g2g? [Re: maddchef]
#23377510 - 06/24/16 01:16 PM (7 years, 7 months ago) |
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I just do them in my SAB and do not remove the foil until ready to G2G... so far no problems...
-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Anyone wipe the top of a master grain jar with is before a g2g? [Re: FreeWorldOrder]
#23377662 - 06/24/16 01:59 PM (7 years, 7 months ago) |
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He's talking about the donor jar (master). Leaving the foil on that one can trap moisture on your filter and cause contams, it could also hinder GE if it's wrapped tight enough.
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FreeWorldOrder


Registered: 12/24/13
Posts: 2,002
Loc: Indiana, USA
Last seen: 8 days, 16 hours
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Re: Anyone wipe the top of a master grain jar with is before a g2g? [Re: Supalemonhaze]
#23381424 - 06/25/16 04:47 PM (7 years, 7 months ago) |
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-------------------- "They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin Lets Grow Mushrooms Videos PastyWhyte's Easy Agar TEK Agar's Liquid Inoculant TEK
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