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OfflineDrMushroom
Human Farmer
I'm a teapot

Registered: 04/28/12
Posts: 722
Loc: Australia
Last seen: 23 days, 8 hours
best ways to quickly check spore syringes or LC for contamination
    #23375049 - 06/23/16 08:00 PM (7 years, 7 months ago)

Im in a bit of a pickle here because i cant trust my syringes.
the syringes i used last time without fail had a few contaminations.
At the same time much much older syringes made sloppily and formed a filthy brown mess of spores and myc in yellowish water that even contained windex, has been very successful and produced no contamination.
I made many syringes in one go from different species, pesa, pf classic, orissa and creeper. All creepers contaminated straight away so i went back to the old syringe instead and refilled with new water which i believe is reponsible for the arbitrary black mold contaminations. 1 Pesa contaminated with something that it seems germinated in the syringe, wheras nearly 3 weeks have passed and the other 2 jars have yet to show any growth, be it mold, myc or bacteria.
All but one orissa contaminated with black mold which all suddenly appeared after nothing for weeks along with myc growth in all but quickly killed off by the mold.
1 creeper contaminated, also with black mold, the rest are fine and almost finished colonizing, with one pinning even. This syringe i used for my previous batch however which had a total success rate.
The PF classic has remained dormant for weeks also but finally started growing, none contaminated.
Golden teacher was untouched and not refilled with new water.

Keeping track of the mold types and everything is tedious and frustrating, because, the black mold contamination must come from the creeper syringe and must have tainted the water i used to refil almost empty syringes to try again after the first batch failed.

Dont try to form any assumptions though based on what ive said here.

Point is, its taken a long time and many resources to determine if a spore syringe is actually contaminated or not. My technique is much much cleaner and nitpicky than before, so its demotivating to see so much failure and uncertainty.

I want to know, how can i absolutely determine if a syringe is clean or not, the quickest, cheapest way?
I want to be able to rule out certain things so i can find the contam leaks and fix them.

My technique sometimes has produced perfect results with next to no sterile method, even an open air inoculation of jars/agar from said syringe, my reliable golden teacher syringe was made in open air, and also the worst ever results where ive taken ridiculous measures to ensure cleanliness.

Somethings not good enough and i need to find out what.
everything is in question now so i cant go ahead and try out things like liquid cultures, and im uneasy about making bulk amounts of anything


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InvisibleSnazz
Polymath
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Registered: 11/24/15
Posts: 1,584
Loc: Canada Flag
Re: best ways to quickly check spore syringes or LC for contamination [Re: DrMushroom]
    #23376555 - 06/24/16 08:13 AM (7 years, 7 months ago)

Incubation in a petri dish is the only true way to check what's inside.  Dealing with the microbe world.


Would be nice if some indicator + black light could help, but typically you have a modded microbe in those cases


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