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wayfaytoolive23
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help
#23375003 - 06/23/16 07:45 PM (7 years, 7 months ago) |
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I inoculated some jars today i wiped the niddle tip with a alcoholpad And then flamed the niddle it like spirited and made like a poping noise Is that normal pls help
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dankington
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yeah. the needle was hot. that's why we depress the plunger very carefully. Get one drop out, and it's ready to use.
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wayfaytoolive23
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I was just triping out so thats normal so what your saying is for next time Let a very small drop out and then the rest
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Canc3r_Dav3
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Quote:
wayfaytoolive23 said: I was just triping out so thats normal so what your saying is for next time Let a very small drop out and then the rest
what he was saying was after you heat the needle barely push the needle so a little lc will come out which results in the needle cooling down.
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wayfaytoolive23
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Thanks guys that helped alot
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Canc3r_Dav3
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no prob, good luck on your grow
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stupididiot
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it's just because the needle had spore liquid in and the heat caused the pressure to rise, with the stopper in the syringe not letting it go backwards, resulted in it pooting out the needle ("science" (no problem))
i would definately still/just make sure you go through the dry verm layer and let it drip down the sides though,
like considering we are causing spores "thermal death" in the needle, i don't really see how squirting more out is going to be of much help as 1)you are prolonging the time the needle can come into contact with pathogens and 2)you would be achieving the same cooling effect (presumably..) whilst increasing the amount of spores available when going through the dry verm / squirting right where you want the spores to go... (with less time for #1).
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dankington
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What are you on about? That's why we use as little spore solution as possible. Flaming the needle and squeezing out a drop as it hisses and sputters is part of proper sterile technique.
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MycoLoopology
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umm definitely squirt a little bit out to cool the needle down. personally i forgot once(too stoned off a dab or 4 i took beforehand) and i stuck the needle right into the polyfil, still red hot lol. polyfil melted all over the needle. i just flamed it back off and let it cool so i dont think there was much damage done, but still; not ideal.
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mupetmower
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squirting a drop or two out to cool the needle will be much faster than waiting for it to cool on it's own. which means it give less time for it to sit there after flame and before inoc.
like dank said, its just a part of good sterile technique, and the hissing is normal.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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stupididiot
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relatively speaking; how is squirting a bit out into the ambient air, any difference from squirting a bit out into the ambient temperatured mycelium food ?.
please ?
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LocN9ne
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Because the red hot needle can kill spores (in theory) But there are so many it probably doesn't matter... By why chance it...
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stupididiot
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???
i will rephrase in that case:
what is the difference between dead spores on your carpet, and sterile dead spores in the mycelium food (worse case scenario)
??
(again baring in mind, if you are squirting things away, into the air, you are increasing the chances of bacterial spores landing on the needle ?)
(....please don't answer with "you squirt them down" "i use a SAB" etc; that is not answering the question)
??
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dankington
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Because spores aren't sterile, you would just invite more contams. Less is better with spores. Do what you want, though.
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LocN9ne
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Quote:
stupididiot said: ???
i will rephrase in that case:
what is the difference between dead spores on your carpet, and sterile dead spores in the mycelium food (worse case scenario)
??
(again baring in mind, if you are squirting things away, into the air, you are increasing the chances of bacterial spores landing on the needle ?)
(....please don't answer with "you squirt them down" "i use a SAB" etc; that is not answering the question)
??
Ohhhhhhh... You don't HAVE to cool the needle first at all really... That is more for if you are doing agar because you only need one drop, so you don't want to chance that one drop being 50% burnt spores.
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desr
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Quote:
stupididiot said: ???
i will rephrase in that case:
what is the difference between dead spores on your carpet, and sterile dead spores in the mycelium food (worse case scenario)
with a red hot needle, you might as well be shooting blanks, so -just liquid.. and if you made your substrate to field capacity, more liquid could negatively affect your moisture content. especially for those using pf jars.
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stupididiot
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Re: help [Re: desr]
#23377704 - 06/24/16 02:16 PM (7 years, 7 months ago) |
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what is the needle capacity ? (vs the "tank")
like 0.1 ml or something ?
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LocN9ne
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Re: help [Re: desr]
#23377736 - 06/24/16 02:28 PM (7 years, 7 months ago) |
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The amount of Extra solution is negligible...
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Supalemonhaze
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Am I the only one that finds the OP funny? Seriously,
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mupetmower
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haha yeah i did too.
like... what else could that have been besides normal.
I can actually sorta imagine OP, flaming his needle and hearing the hiss and freaking out haha
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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Inocuole
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"niddle" "poping" "spirited"
But he got inoculated right.
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dankington
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whatever innocule. Dick.
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Inocuole
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Whatever you say darkington..
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dankington
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Dude hasn't even been back since we answered the question. I guess he got spirited away.
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Inocuole
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Gotta admit though, the image of somebody getting legitimately freaked out and bug eyed and shit about the hissing/spitting of the syringe needle is pretty hilarious.
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Canc3r_Dav3
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Quote:
mupetmower said: I can actually sorta imagine OP, flaming his needle and hearing the hiss and freaking out haha
Lol freaks out and throws the syringe cause he thinks the pop was it about to explode.
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Supalemonhaze
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Quote:
Inocuole said: Gotta admit though, the image of somebody getting legitimately freaked out and bug eyed and shit about the hissing/spitting of the syringe needle is pretty hilarious.
Very true
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stupididiot
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Quote:
LocN9ne said: The amount of Extra solution is negligible...
.
if it is negligible ?
but the contaminination risk involved with doing so is very real >
then 1) why is it good advice (being negligble, yet providing risk of contamn)
and 2) why the need to navigate to my profile and give me a 0 rating ?
??
they are your words ? > were you angry with yourself and trying ot take it out on me or something ?
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dankington
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you do understand what Loc meant, right? Negligible as in: the losses from the drop or two expelled from the hot needle are negligible.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



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--------------------
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Inocuole
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Quote:
stupididiot said: and 2) why the need to navigate to my profile and give me a 0 rating ?
To be fair, it's only one click away from any of your recent shitposts.
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Canc3r_Dav3
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Lolz
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stupididiot
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...now being stalked by more people.
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you know, when you mindlessly follow rhetoric ? > like going around the houses, squirting liquid into the air "because you heard it [from somewhere] (no sources cited)" thus "do it"
and somebody asks, "why are you going around the houses ?; i don't understand"
and then people reply because that's the way "you do it YOU IDIOT"
> it's not answering the question ?
(presumably because you guys will be really pissed if you open your minds to the fact you may be squirting water from an implement into the air for no particular reason ?.)
like no one has yet answered, objectively WHY DO YOU SQUIRT IT OUT ?
only "the pros do it so i do it toooooooo"
(newsflash > "the pros" maybe bullshitters, and you guys maybe silly).
again; if the difference in quanity is 'neglible' (dunno if that guy is a "pro") (do you need to rage about in people profiles to be pro ?).
and waving about needles you have just sterilised is a bad idea (unless anyone would suggest otherwise ?)
and no one tells me why it is of benefit to squirt into the air (but i know factually it is a bad idea for contamns)
...then
i don't really know what to say ?
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dankington
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who's walking around their houses with syringes you stupid, goddamned idiot?
We aswered your question. It's not our faults you're such as stupid goddamned idiot. Even you recognize the fact!
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stupididiot
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where did you answer the question objectively ? (i.e. without unrelated / negligable information etc)
??????
Edited by stupididiot (06/25/16 05:47 PM)
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dankington
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how did I not? Do you even understand your question, or are you too stupid?
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stupididiot
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yes you said you did, but i can't find it and don't believe you anyway >
so now i am asking you to provide "evidence" of your claim ?
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dankington
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a suspension inside the syringe is as sterile as it's going to be (be it aseptic or not). You are trying to dispel outside contaminants. The needle is open. So, we heat the open needle to around 1000º or whatever, and dispel the solution to cool it. What's there to get?
we're trying to minimize our vectors. Stupid idiot.
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stupididiot
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what ????
you wrote something new ????
please provide evidence of your claim ? > direct me to where the question was answered like you say ?
----------------------
and please read > the temp loss can be achieved with NEGLIGABLE loss of liquid by going into the jar, without the contamn risk >
therefore your new BS is not answering the question.
> the same result can be achieved, without the contamn risk with negligable loss of liquid thus you still have not produced a valid answer > SO WHY DO YOU SQUIRT OUT ?
> still waiting ????
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dankington
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what evidence is needed? It's pretty much common sense at this point. Unless of course you're a stupid goddamned idiot.
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stupididiot
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how is needlessley doing something, that it more complicated and less effective "common sense" ???.
you don't have to provide a quote etc > but you should know you are full of shit for making acusations and not backing them up > a pointless person who doesn't contribute ?
like right up in your personailty to your core is pointless ?, there is no point if you just going around "blah blah" (lying, then when questioned, just type BS ?
> do i have to go from this thread to ?
you are not going to leave or answer are you ? just post BS ?
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Inocuole
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What is the fucking question? I will answer it and everyone will shut the fuck up.
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dankington
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what is more effective? I think you're being an asshole for the sake of it at this point.
You really think I don't contribute? You may not like reason slapping you in the face, but you gotta accept it. Unless you're too much of a stupid idiot to understand what I'm saying.
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Inocuole
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What is more effective between what options? Heat the fuckin needle, eject a drop to cool the needle, continue with your inoculation using sterile needle. Inside an SAB or in front of a flow hood, of course. There is no other more effective technique.
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Supalemonhaze
Spore syringe hater.



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Quote:
stupididiot said:
and no one tells me why it is of benefit to squirt into the air (but i know factually it is a bad idea for contamns)
I'm interested in how someone who probably never even grew shrooms actually knows more than the people who do this on the regular. I gotta ask, how exactly is it a "factually bad idea for contams?" If anything, it's the exact opposite, hence why everyone said to squirt outside the jar.
You're about to get more neg rates I reckon, just about had it with this idiocy.
This is so fucking obvious that it's entirely due to you being so simple-minded. As you should already know, spore solutions are not ever clean. As others have already mentioned in the beggining of the thread, when using spore solutions, less is more. So with that in mind, would you rather have more spore solution in your jar, or less? Let me re-phrase, do you want a higher chance of contams, or less?
Jesus, even my 6 year old nephew could have made the math to this one. You really are something special(not a compliment) to argue about something so simple and elemantary. And you say your name "is just a name." The more you post, the more you do come out like a damned idiot.
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stupididiot
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no its YOU GUYS AGAIN
> IF THE ACTION IS POINTLESS (considering the increased RISK OF CONTAMN and the offset in liquid is *negligible)
* adjective 1. so small, trifling, or unimportant that it may safely be neglected or disregarded:
/\ for you idiots this MEANS "to cool it down" is NOT VALID REASONING FOR DOING THE ACTION:
therefore:
WHY ARE YOU PEOPLE GOING AROUND DOING POINTLESS THINGS FOR NO REASON ????
..........
....
...
..
.
?
still not answered, 3 pages of bullshit from you guys, well done, you should pat yourselves on the back / circle jerk / whatever.
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stupididiot
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or is there a reason ?
guessing there isn't ?
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dankington
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I think you just don't get it. You know, because you're such a stupid fucking idiot.
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dankington
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The bottom line is, we can't teach you grade school science. If you're a 2nd grade drop-out, it's not our problem.
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stupididiot
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yes i knew you weren't going to go......,
very predictable, you morons;
hope the "pros" don't inform you guys that jumping off bridges etc is a good idea etc etc....
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dankington
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:strawmanargument:
instead of trying to insult me, why not ask what exactly you're not getting, idiot? I'd love to help a stupid idiot just be an idiot. You know, a step up.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
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Quote:
stupididiot said: yes i knew you weren't going to go......,
very predictable, you morons;
hope the "pros" don't inform you guys that jumping off bridges etc is a good idea etc etc....
Ok, let's do this another way. Why is it not a good idea to squirt outside the jar? I'm geniunely curious.
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mupetmower
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i think you should just give up on this guy. i really dont see how he doesnt get it. at this point i really do think he is just trolling. question was answered more than once. lets just leave this guy to his own devices. he either isnt going to understand, or he doesnt want to. kinda think it's the latter.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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MycoLoopology
Eye's wide open



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Quote:
Inocuole said: What is more effective between what options? Heat the fuckin needle, eject a drop to cool the needle, continue with your inoculation using sterile needle. Inside an SAB or in front of a flow hood, of course. There is no other more effective technique.
answer is here.
-------------------- Current Grow Log ^^My first grow log <3^^ Wish me luck.
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