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wayfaytoolive23
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Registered: 07/31/13
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help
#23375003 - 06/23/16 07:45 PM (7 years, 7 months ago) |
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I inoculated some jars today i wiped the niddle tip with a alcoholpad And then flamed the niddle it like spirited and made like a poping noise Is that normal pls help
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dankington
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yeah. the needle was hot. that's why we depress the plunger very carefully. Get one drop out, and it's ready to use.
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wayfaytoolive23
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I was just triping out so thats normal so what your saying is for next time Let a very small drop out and then the rest
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Canc3r_Dav3
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Quote:
wayfaytoolive23 said: I was just triping out so thats normal so what your saying is for next time Let a very small drop out and then the rest
what he was saying was after you heat the needle barely push the needle so a little lc will come out which results in the needle cooling down.
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wayfaytoolive23
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Thanks guys that helped alot
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Canc3r_Dav3
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no prob, good luck on your grow
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stupididiot
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it's just because the needle had spore liquid in and the heat caused the pressure to rise, with the stopper in the syringe not letting it go backwards, resulted in it pooting out the needle ("science" (no problem))
i would definately still/just make sure you go through the dry verm layer and let it drip down the sides though,
like considering we are causing spores "thermal death" in the needle, i don't really see how squirting more out is going to be of much help as 1)you are prolonging the time the needle can come into contact with pathogens and 2)you would be achieving the same cooling effect (presumably..) whilst increasing the amount of spores available when going through the dry verm / squirting right where you want the spores to go... (with less time for #1).
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dankington
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What are you on about? That's why we use as little spore solution as possible. Flaming the needle and squeezing out a drop as it hisses and sputters is part of proper sterile technique.
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MycoLoopology
Eye's wide open



Registered: 06/12/16
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umm definitely squirt a little bit out to cool the needle down. personally i forgot once(too stoned off a dab or 4 i took beforehand) and i stuck the needle right into the polyfil, still red hot lol. polyfil melted all over the needle. i just flamed it back off and let it cool so i dont think there was much damage done, but still; not ideal.
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mupetmower
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squirting a drop or two out to cool the needle will be much faster than waiting for it to cool on it's own. which means it give less time for it to sit there after flame and before inoc.
like dank said, its just a part of good sterile technique, and the hissing is normal.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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stupididiot
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relatively speaking; how is squirting a bit out into the ambient air, any difference from squirting a bit out into the ambient temperatured mycelium food ?.
please ?
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



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Because the red hot needle can kill spores (in theory) But there are so many it probably doesn't matter... By why chance it...
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stupididiot
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???
i will rephrase in that case:
what is the difference between dead spores on your carpet, and sterile dead spores in the mycelium food (worse case scenario)
??
(again baring in mind, if you are squirting things away, into the air, you are increasing the chances of bacterial spores landing on the needle ?)
(....please don't answer with "you squirt them down" "i use a SAB" etc; that is not answering the question)
??
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dankington
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Because spores aren't sterile, you would just invite more contams. Less is better with spores. Do what you want, though.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
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Quote:
stupididiot said: ???
i will rephrase in that case:
what is the difference between dead spores on your carpet, and sterile dead spores in the mycelium food (worse case scenario)
??
(again baring in mind, if you are squirting things away, into the air, you are increasing the chances of bacterial spores landing on the needle ?)
(....please don't answer with "you squirt them down" "i use a SAB" etc; that is not answering the question)
??
Ohhhhhhh... You don't HAVE to cool the needle first at all really... That is more for if you are doing agar because you only need one drop, so you don't want to chance that one drop being 50% burnt spores.
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desr
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Registered: 10/04/11
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Quote:
stupididiot said: ???
i will rephrase in that case:
what is the difference between dead spores on your carpet, and sterile dead spores in the mycelium food (worse case scenario)
with a red hot needle, you might as well be shooting blanks, so -just liquid.. and if you made your substrate to field capacity, more liquid could negatively affect your moisture content. especially for those using pf jars.
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stupididiot
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Re: help [Re: desr]
#23377704 - 06/24/16 02:16 PM (7 years, 7 months ago) |
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what is the needle capacity ? (vs the "tank")
like 0.1 ml or something ?
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



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Re: help [Re: desr]
#23377736 - 06/24/16 02:28 PM (7 years, 7 months ago) |
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The amount of Extra solution is negligible...
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Q&A US vs. THEM The more I learn, the less I know.
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Supalemonhaze
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Am I the only one that finds the OP funny? Seriously,
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mupetmower
Mower of Muppets



Registered: 03/29/16
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haha yeah i did too.
like... what else could that have been besides normal.
I can actually sorta imagine OP, flaming his needle and hearing the hiss and freaking out haha
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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