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Offlinezathras
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Perception7]
    #23402240 - 07/01/16 08:04 PM (7 years, 6 months ago)

I like reading your logs, and am excited to see where this one goes! I'm messing around with PE right now myself. Those plates are looking nice and you've got me craving a mycology fridge.


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OfflineMycologist217
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: zathras]
    #23402674 - 07/01/16 11:09 PM (7 years, 6 months ago)

Thanks a lot, Zathras!

I inoculated my first 7qts of birdseed tonight with the tester LC I made 9 days ago...

This really is the hard part for me....it's really the only time during the grow when I just won't know how things are gonna go....my LC is either clean and ready to do some work shooting up WBS, or its dirty and not only do would I have seven useless jars of WBS on my hands, but also be back to square one making an inoculant.....worse comes to worse we will shift our learning objectives and do G2G after making master jars out of the plates we expanded our culture to last week....but obviously we are hoping for clean LC so we can get our tubs spawned in the next two weeks!

Thanks for the support so far guys!

As for those of you growing PE from MS, good luck! I hope you get great results from your grow to capture some genetics! The M217-A isolate I'm working with was derived entirely from a fruit produced in a first generation MS grow! You can find the log for that grow HERE....the last time I used spores....


--------------------
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OfflineMycologist217
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
    #23405808 - 07/03/16 12:22 AM (7 years, 6 months ago)

There is clean rhizo mycelium against the glass of one jar...obviously all will be revealed in time....but I have no reason not to be pretty stoked right now.....hurry up jars!

In other news....watching the liquid culture colonize is kind of crazy....as the mycelium colonizes the honey in suspension the solution becomes less and less Amber in hue. I observed similar results when I used the same LC recipe in January. I know realistically this must be a result of the honey/nutrients being consumed....which leads me to my next observation:

So often, we'll see it on these forums that there are little to no visual indication of a dirty liquid culture, but I think I must disagree....at least in the case with the liquid culture I prefer of 85%water 15% Honey and added mycelium+agar wedge....the rhizomorphs are apparent to the naked eye when looking at the growths in the LC.....I admit that adding a stir device...perhaps a small marble....would make breaking up these colonies easier and protect the integrity of the gas exchange filter, as it's has certainly gotten wet....I am giving my faith to the fact that the filter is almost an inch long and that the vanguard layer on the lid has certainly remained dry....alas...the rhizomorphs are apparent in the solution...additionally....I still have the LC I actually used in January and there is a red flake of silicon that a syringe needle certainly knocked off the SHIP....but even six months later it remains bright red....I feel like scum...bacteria....might either land and rest on the silicon forming a discoloration...idk though because the cube myc certainly doesn't land and rest on it....however, I must say I believe a contamination WOULD indeed make itself visually present in this LC....especially after a significant amount of time (I.e. Six months)

I'll get some pics up when the jars get a little photo worthy....gonna be an exciting week that's for sure!


--------------------
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Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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OfflineKenetic
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
    #23405870 - 07/03/16 12:55 AM (7 years, 6 months ago)

Looks like fun!


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Todo Cambia
   

               
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OfflineMycologist217
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Kenetic]
    #23408138 - 07/03/16 07:14 PM (7 years, 6 months ago)

5/7 jars have visible mycelium growth! Pretty thrilled! Gonna get bird seed soaking tonight to pressure cook and shoot up tomorrow


--------------------
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AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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OfflineMycologist217
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
    #23411923 - 07/04/16 09:19 PM (7 years, 6 months ago)

Second set of seven jars are sterilizing!


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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InvisiblePastywhyteMDiscord
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
    #23411967 - 07/04/16 09:33 PM (7 years, 6 months ago)

Always like to see a good PE grow.


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OfflineMycologist217
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Pastywhyte]
    #23412279 - 07/04/16 11:33 PM (7 years, 6 months ago)


5/7 jars from the first set are looking like this....super thrilled on yet another clean LC! I used about 1.5ml LC per WBS jar....I would use more but I don't want to fill the syringe more than once per inoculation round. Hoping these fully colonize within 10-14 days.


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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OfflineMycologist217
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
    #23413403 - 07/05/16 11:05 AM (7 years, 6 months ago)

Got the second round inoculated and the third round soaking before sterilization tonight.

I want to take a second and toot my own horn. Liquid Culture catches a pretty shady rep on these forums, and I don't like it. I consider myself to be fairly proficient in them now, as I've been consistently producing clean liquid cultures all year, and I sit wish they would catch on a bit more....it reduces labor by so much....you wait for a clean agar wedge...drop it in your LC...colonize....and inoculate your grains....no g2g...i encourage you all to at least try it out, and allow me to plug my own research here....give my Water/Honey LC a try....and the lid build....you can find my LC tek HERE


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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InvisiblePastywhyteMDiscord
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
    #23413461 - 07/05/16 11:26 AM (7 years, 6 months ago)

I don't think it's LC persay that gets a bad rep. Moreso the attitude that it's a shortcut. Spores to LC is frowned upon as is tissue biopsy but even those things can be made to work provided careful testing is done before committing to large quantities grain.

However I will say that I personally dislike simple sugar LC and probably dislike honey most of all. Too much sediment, not enough upside to risk the culture getting lazy. Malt extract is far better.


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InvisibleSnazz
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
    #23413482 - 07/05/16 11:34 AM (7 years, 6 months ago)

They get a bad rep cuz noobs like me use spore syringes to make LC for their first attempt at the hobby. 


Petri dishes and practice with sterile technique makes all the difference.


Just trying them out again after reading your Tek


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Offlineguyute22
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Registered: 07/27/15
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Snazz]
    #23413551 - 07/05/16 12:00 PM (7 years, 6 months ago)

:sun::popcorn::sun:


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OfflineMycologist217
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: guyute22]
    #23413962 - 07/05/16 02:25 PM (7 years, 6 months ago)

I don't like the sediment one bit either, but I can't complain when the hardware store sells all natural organic honey cheap


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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OfflineMycologist217
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
    #23418699 - 07/06/16 10:34 PM (7 years, 6 months ago)

I shook the seven jars from round one yesterday morning, they have all recovered including the two that were not yet showing growth....looking good....

3/7 jars inoculated on Tuesday morning are already showing healthy growth....the LC is hitting its prime I think....ugh....I got distracted by the female species yesterday and did not get the third round of seed sterilized until this evening after a staggering 29 hour soak.....fuck.....but oh well....got round four's seed soaking now so I can sterilize it in the morning after round three gets noc'd up. Ideally I can inoculate round 3 and 4 tomorrow and be on schedule to finish the sixth and final round on Saturday....crazy by then I should be looking at round one being close to done...lots of labor this grow.....hopefully I get 6-7 bad ass Tubs out of it!


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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OfflineMycologist217
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
    #23421994 - 07/08/16 12:17 AM (7 years, 6 months ago)

Round 4 has been inoculated.
Round 5 is sterilizing now for inoculations in the morning.
Round 6 is soaking to get simmered and sterilized in the morning.....

Almost done with birdseed prep....pics will be coming much much more frequently after all 42 jars have been inoculated!

Almost didn't have enough birdseed on hand....but I think we'll barely fill round 6's seven quarts! Ready for the wait I between inoculation and spawning.....I think my colonization closet is a little too low in temp...I'll be raising temps in there once all jars are on board....all jars from rounds 1 & 2 are showing plenty of healthy rhizomorphic mycelium!

Just want to reiterate the same jar of LC is being used in every inoculation, as well as a rotation between two different syringes is being employed. The syringes get sterilized (empty, wrapped in foil) with the birdseed for each round!

POKEMON GO


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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Offlineguyute22
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
    #23422528 - 07/08/16 06:43 AM (7 years, 6 months ago)

i am often tempted to raise temps, but every time I do, comtams, especially mold, become more frequent.  If temp is above 67F, I would not touch it.


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InvisibleSupalemonhaze
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: guyute22]
    #23422611 - 07/08/16 07:27 AM (7 years, 6 months ago)

High 60's is my favourite temp. Anything above 72F annoys me. It's 85F currently in my grow room, safe to say I'm not a happy camper. Did an oyster basket the other day and I put it in a tote to colonize. Checked up on it a few days later and the heat in the tub was uncomfortable on my hand. That shit was easily 95F+. Needless to say, that laundry basket is now green and in a garbage bag.

As for LC, I've had success with it in the past but lately I've had nothing but fails with it. I like it but it's not worth the trouble for me. It's kind of funny because my sterile tek is the best it's ever been atm and my last few attempts with LC crashed and burned miserably.

Malt extract still gets me sediment in my LCs, just as much as a good brand honey. It doesn't bother me much though because the sediment is the first thing the mycelium devours. The mycelium literally grows through the solids, locking them within it and slowly eats them. By the time it's grown enough to use, half of the sediment would be already gone. It's annoying but easy enough to get rid of. I still prefer malt LC though, the solution is clearer than any honey LC I've ever made.


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OfflineMycologist217
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Supalemonhaze]
    #23425303 - 07/09/16 12:22 AM (7 years, 6 months ago)

Got all 42 target grain jars inoculated...glad to be done with that for a while, and will say next time I do a grow log I think it will feature an alternative spawn media too WBS....I'm kind of tired of it.

I inoculated one jar from round 5 with 10mls of LC. We'll see how that goes.
I inoculated all jars in round 6 with 3-4mls LC. We'll see how that goes.

All growth so far across previously inoculated jars appears healthy and Round 2 is recovering from a shake nicely....hope colonization of these grains doesn't take more than a couple weeks!




--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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Offlinetripdawg420
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
    #23425310 - 07/09/16 12:25 AM (7 years, 6 months ago)

:headbanger:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

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http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:


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OfflineMycologist217
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Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: tripdawg420]
    #23428025 - 07/09/16 11:26 PM (7 years, 6 months ago)

Everything's definitely going according to plan,...I suspect I am going to get through with all 42 jars most likely..,,the culture is taking a few days to get onto the birdseed, but once it does it is really doing work. It has a distinctly clean look to it (the mycelium) and I'm really just glad to be able to kick back and relax for another week or more before I have to start spawning monotubs!

Here's what we are working with now:


I expect some jars from round 1 will start finishing up by next weekend.

The jar I shot 10mls into is already showing a bit of seed colonization....


--------------------
My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M
AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

Check out my Retail Gourmet Mushroom Farm!

Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.


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