|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
|
COMPLETED SUCCESSFUL GROW--Interactive Grow Log SUMMER 2016~~ PENIS ENVY M217-A 2
#23374414 - 06/23/16 04:55 PM (7 years, 7 months ago) |
|
|
Greetings again Shroomerites!
We're back for our annual summer project, and of course that means a new real time grow log, but this time I want to modify the structure to be more conducive to discussion. That is we are going to treat this real time log also as a forum for the open discussion of cultivating the Penis Envy. I a, curious to know how my colleagues tackle this beast differently than I do, and I just want to see the dicks you all have going on, so let's gear up, strap In, and get ready to:
~BRING AN ISOLATE PLATE OUT OF FIVE MONTHS COLD STORAGE (Our very own M217-A) ~TRANSFER PRESERVED ISOLATE TO NEW PLATES ~PREPARE A MYCOLOGIST217 WATER/HONEY LC WITH PRESERVED GENETICS ~INOCULATE 42 GRAIN (WBS) JARS WITH PREPARED LIQUID CULTURE ~FACILITATE THE COLONIZATION AND FRUITING OF 6-7 MONOTUBS
LEARNING OBJECTIVES: -Successfully use genetics that have been in long term storage to perpetuate a "large" scale bulk grow. -Observe physical difference in harvests across different MONOTUBS spawned with identical genetics. -Maintain a high level of contamination free mycology. -Demonstrate an advanced practical knowledge of bulk cultivation technique as it applies to the PC Penis Envy. -Provide an Admirable example of such applications as Agar Plate Work (cloning, strain isolation, sterile technique), Liquid Culture, and Frank Horrigan inspired MONOTUBS!
WHERE DO WE START? Okay! So I started this project officially a couple days when I pulled this plate out of refrigeration and prepared some new agar plates:
 Whereupon yesterday morning I made the actual transfers from the original isolate plate onto the four new plates as well as a tester LC:

My theory is that if my four plates come out clean....the LC should also be clean and therefore ready to use, without having to wait for these plates to grow out and then making another LC and having to wait on that....
So that's where I am now....like I said I'm super curious to see the penis envy you guys are working with, so let the discussion begin!
If you are curious about my history and credentials growing Penis Envy, please refer to links provided in my signature! Let's a have a great summer of magical dick!
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
Edited by Mycologist217 (08/25/16 09:15 AM)
|
MycoLoopology
Eye's wide open



Registered: 06/12/16
Posts: 518
Last seen: 2 years, 7 months
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
#23374499 - 06/23/16 05:25 PM (7 years, 7 months ago) |
|
|
cant wait to see how all this pans out good luck buddy!!
-------------------- Current Grow Log ^^My first grow log <3^^ Wish me luck.
|
jerrylove
little bit further then I was



Registered: 06/19/09
Posts: 602
Loc: shakedown street
Last seen: 1 year, 4 months
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: MycoLoopology]
#23374533 - 06/23/16 05:37 PM (7 years, 7 months ago) |
|
|
Oh hell ya I'm gonna keep an eye on this one
--------------------
THOSE WHO KNOW DONT TALK! THOSE WHO DONT KNOW CANT TALK. (Timothy Leary) "Funny thing about weekends when your unemployed they don't mean quit so much except you get hang out with your workin' friends" Les Claypool
|
SloppyJoseph
Non-sporalator


Registered: 12/08/15
Posts: 8,511
Loc:
Last seen: 3 years, 7 months
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: MycoLoopology]
#23374537 - 06/23/16 05:38 PM (7 years, 7 months ago) |
|
|

Just straight up, jealous if this comes out as good as last time. if you dont mind me asking, how many tubs you trying to do this round?
-------------------- AMU Q&A
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: SloppyJoseph]
#23374611 - 06/23/16 05:57 PM (7 years, 7 months ago) |
|
|
6-7 monos....will be inoculating 7qts of birdseed a day for 6 days straight....hoping to use 6 qts per mono. Doing 7 per day to give me one bonus jar to support a 15% contamination rate
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
#23374625 - 06/23/16 06:03 PM (7 years, 7 months ago) |
|
|

I gotta pull up a chair for this
|
tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,071
Loc: illinois
Last seen: 18 hours, 36 minutes
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: dankington]
#23374678 - 06/23/16 06:16 PM (7 years, 7 months ago) |
|
|
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: SloppyJoseph]
#23375000 - 06/23/16 07:44 PM (7 years, 7 months ago) |
|
|
Quote:
SloppyJoseph said:

Just straight up, jealous if this comes out as good as last time. if you dont mind me asking, how many tubs you trying to do this round?
Call me a pompous prick...Hahahaha.....but I'm actually hoping for 5lbs out of 6-7 tubs.....hahahahaha
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
SloppyJoseph
Non-sporalator


Registered: 12/08/15
Posts: 8,511
Loc:
Last seen: 3 years, 7 months
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
#23375004 - 06/23/16 07:45 PM (7 years, 7 months ago) |
|
|
You're probably gonna get it too if you spawn 7... You pompous prick
-------------------- AMU Q&A
|
magicMerlin



Registered: 01/26/12
Posts: 617
Loc: Toronto
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: SloppyJoseph]
#23376977 - 06/24/16 10:32 AM (7 years, 7 months ago) |
|
|
I liked your last grow log, hopefully you have just as much or more success this time!
-------------------- My favorite threads
|
cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 9 days
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: magicMerlin]
#23377300 - 06/24/16 12:11 PM (7 years, 7 months ago) |
|
|
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: cronicr]
#23377408 - 06/24/16 12:43 PM (7 years, 7 months ago) |
|
|
The transfers from the old plate are all taking to their new plates quite nicely....everything is definitely still alive...hoping the LC I prepared takes and colonizes....don't want to be waiting around for more than a week...in the meantime....I do have a 5month old batch of LC still.....hahaha I mostly want to leave it alone but it looks promising actually.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
#23378786 - 06/24/16 09:22 PM (7 years, 7 months ago) |
|
|
Just going to take a second to explain that the culture I am working with is a monostrain isolate that I isolated away from a clone transfer I took back in December. So while absolute correct terminology for my culture is strain or isolate, I find it easiest to refer to it as my clone....SO....
The clone plates are definitely alive! With a couple of them having already hit their new agar! Pretty exciting for me, as it indicates a clean lab session the other morning and would be indicative of a cleanly made Liquid Culture also! So we are waiting for that to get a little bit of Mycelium going, but here are the new clone plates made the other night from a parent that spent five straight months in a mini fridge:

Already thrilled! Thanks for joining the thread guys! Especially glad to see you here, Cronicr, something about you watching my work makes me feel pretty cool  
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
#23381872 - 06/25/16 07:13 PM (7 years, 7 months ago) |
|
|
probably my nicest couple of plates ever.....a couple more days of colonizing before these hit the fridge

I might make an LC out of the super pretty one.
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
jerrylove
little bit further then I was



Registered: 06/19/09
Posts: 602
Loc: shakedown street
Last seen: 1 year, 4 months
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
#23382004 - 06/25/16 08:01 PM (7 years, 7 months ago) |
|
|
Dude I'm coming to your house for a penis and spaghetti diner when you get this one done!
--------------------
THOSE WHO KNOW DONT TALK! THOSE WHO DONT KNOW CANT TALK. (Timothy Leary) "Funny thing about weekends when your unemployed they don't mean quit so much except you get hang out with your workin' friends" Les Claypool
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: jerrylove]
#23385313 - 06/26/16 07:38 PM (7 years, 7 months ago) |
|
|
So all four plates did major work colonizing today, and the pretty one is about ready to go into storage.....I want to make an LC out of it at some point, but not until after I find out the viability of the one I made along side these plates. It is definitely looking like we have mycelium in our Water/Honey LC

I guess this really is the reason I prefer liquid culture so much, I mean, I only had to take a single wedge transfer to make it, and now I'll have enough inoculant to make 250 WBS jars without having to wait on master grain jars for G2G (which I've never attempted anyway) but now I can see that going that route would virtually be doubling my spawn colonization period....that's my 2cents on that. What do you guys think?
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
Edited by Mycologist217 (06/26/16 07:40 PM)
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
#23392114 - 06/28/16 08:10 PM (7 years, 6 months ago) |
|
|
I have decided to name this strain the PE M217-A
Is that Savvi? I mean I isolated the strain...no one else has these particular genetics....I can give the culture a name right?
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
SloppyJoseph
Non-sporalator


Registered: 12/08/15
Posts: 8,511
Loc:
Last seen: 3 years, 7 months
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
#23392147 - 06/28/16 08:20 PM (7 years, 6 months ago) |
|
|
Yeah you can name it to put the label on your fridge slant....
-------------------- AMU Q&A
|
Mycologist217
Frank's Disciple



Registered: 02/13/13
Posts: 2,425
Loc: Man of the Moon
Last seen: 1 day, 8 hours
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: SloppyJoseph]
#23400998 - 07/01/16 12:23 PM (7 years, 6 months ago) |
|
|
Birdseed is sterilizing now! We should know by the fourth if our LC is clean!
-------------------- My LC Manual (With custom LC lid Tek) ~~ Required Mycology Supplies ~~ Agar Work Videos ~~ L G M AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE Check out my Retail Gourmet Mushroom Farm! Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.
|
Perception7
Psilocin Technician



Registered: 01/10/16
Posts: 403
Last seen: 6 years, 5 months
|
Re: Interactive Grow Log/Discussion: SUMMER 2016~~ PENIS ENVY [Re: Mycologist217]
#23401208 - 07/01/16 01:46 PM (7 years, 6 months ago) |
|
|
Yeah I will definitely keep an eye on this one - growing my first PE very soon from MS
Edited by Perception7 (07/01/16 01:46 PM)
|
|