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Invisiblelone_psychonaut
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Injector port location
    #23373840 - 06/23/16 01:31 PM (7 years, 7 months ago)

I ordered self healing injector ports.  I've been looking at the layout of the injector port and the gas exchange hole. I've seen numerous videos and pictures of the injector port being on one side of the lid and the gas exchange hole being on the other side.

Isn't it a better idea for the injector port to be in the center of the lid in order to more easily inoculate all the way around the jar? Would a normal mouth lid make any difference?


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Invisiblefilthyknees
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Re: Injector port location [Re: lone_psychonaut]
    #23373885 - 06/23/16 01:50 PM (7 years, 7 months ago)

I would never use ships. I would just lift the lid in an SAB. but if I did use ships, I'd consider that liquid running down the side of the glass will travel farther than if squirted in the center, thus colonization happens faster. so I would put it to one side. I don't think the size of the jar mouth makes a difference.


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Invisiblelone_psychonaut
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Re: Injector port location [Re: filthyknees]
    #23373910 - 06/23/16 01:58 PM (7 years, 7 months ago)

I can understand that but if the hole is in the left side for example won't it be difficult to squirt towards the rights side onto the glass as opposed to shooting the glass from the center?


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Offlineblackout
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Re: Injector port location [Re: lone_psychonaut]
    #23373935 - 06/23/16 02:06 PM (7 years, 7 months ago)

How much do you think you have to squirt in? you would not typically be doing PF cakes with a SHIP, not tradtional ones anyway. If you are squirting in lots of LC you will shake the jar afterwards so it does not matter if it all goes in on one side.

The reason I would have them on opposite sides is so if you make an LC you can tilt the jar and suck out LC from the port while having the filter up and not exposed to the liquid. If the SHIP is on the side of the lid you can drain more from the jar than if its in the middle.


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Invisiblelone_psychonaut
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Re: Injector port location [Re: blackout]
    #23373952 - 06/23/16 02:10 PM (7 years, 7 months ago)

It is for quart rye grain jars. I didn't know about shaking the jars or about sucking out LC


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Invisiblelone_psychonaut
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Re: Injector port location [Re: lone_psychonaut]
    #23374013 - 06/23/16 02:37 PM (7 years, 7 months ago)

I'll continue my research


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Invisiblelone_psychonaut
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Re: Injector port location [Re: lone_psychonaut]
    #23374074 - 06/23/16 03:02 PM (7 years, 7 months ago)

I've seen two different posts from RR where he recommends not shaking the jar after inoculation. So maybe it is better to have a port in the center to equally get all around the jar


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Re: Injector port location [Re: lone_psychonaut]
    #23374080 - 06/23/16 03:04 PM (7 years, 7 months ago)

Quote:

lone_psychonaut said:
It is for quart rye grain jars.



If doing quart jars and if you insist on using MS syringes then most recommend squirting in only a small amount, and recommend squirting it against the side of the jar easily, you do NOT squirt all around the sides of the jar. If doing this it is easier to have the SHIP near one side of the lid, you can put your needle in and aim it towards the jar. You do NOT shake the jar if doing this. Since the SHIP will be one one side you will know where to inspect for growth a few days later too. If you have a SHIP in the centre and squirt in only one place you will have to inspect all around the jar unless you mark it in some way.


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Invisiblelone_psychonaut
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Re: Injector port location [Re: blackout]
    #23374223 - 06/23/16 03:46 PM (7 years, 7 months ago)

Thanks for your input, you've been helpful.
1.) I've been getting a lot of responses about MS syringes being not the best method; is agar the preferred method? I plan on growing one type and then move onto another type and so on so I figured MS would be the best method, if not please give me any advice you have for me... Thank you


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OfflineFreeWorldOrder
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Re: Injector port location [Re: lone_psychonaut]
    #23374241 - 06/23/16 03:50 PM (7 years, 7 months ago)

Quote:

lone_psychonaut said:
I've seen two different posts from RR where he recommends not shaking the jar after inoculation. So maybe it is better to have a port in the center to equally get all around the jar




You ideally need only a few drops of inoculate. If you are squirting it all around the jar then that would be way too much IMO.


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Invisiblelone_psychonaut
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Re: Injector port location [Re: FreeWorldOrder]
    #23374269 - 06/23/16 04:04 PM (7 years, 7 months ago)

I read 1-3 cc for a liquid culture, not sure if that's the same for a spore syringe


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InvisibleInocuole
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Re: Injector port location [Re: lone_psychonaut]
    #23374364 - 06/23/16 04:41 PM (7 years, 7 months ago)

Try half a CC for a spore syringe...  Spores are dirty, less is more.  You can use a lot more LC and LI than you can spores.


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Invisiblelone_psychonaut
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Re: Injector port location [Re: Inocuole]
    #23374380 - 06/23/16 04:46 PM (7 years, 7 months ago)

I'm glad I talked to you guys, I'm not as educated as I thought. I have a ms syringe, I'm researching about liquid cultures and agar for the better method


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Invisiblelone_psychonaut
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Re: Injector port location [Re: lone_psychonaut]
    #23374419 - 06/23/16 04:57 PM (7 years, 7 months ago)

What do you mean by spores being dirty and less being more??


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OfflineBobabouy
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Re: Injector port location [Re: lone_psychonaut]
    #23374476 - 06/23/16 05:19 PM (7 years, 7 months ago)

You should concentrate your research on agar right now. After you have mastered that, the rest is gravy. Search and read and search and read. And boots and pants and boots and pants.


Edited by Bobabouy (06/23/16 05:21 PM)


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InvisibleInocuole
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Re: Injector port location [Re: lone_psychonaut]
    #23374520 - 06/23/16 05:34 PM (7 years, 7 months ago)

Quote:

lone_psychonaut said:
What do you mean by spores being dirty and less being more??




A 100% sterile spore syringe is statistically pretty unlikely.  The more solution you use, the more likely it is that something bad will be included in the inoculation.  Say there's 5 bacterium in your syringe, in different places.  That's probably being pretty modest.  If you put 2-3cc per jar, every jar(all of... 3-5 per syringe :thumbdown:) would be likely to show contamination. If you put 0.5 CC per jar, only 5 out of 20 jars could potentially show contamination from the syringe assuming the other techniques are performed correctly.  Simple probability shit.

Though if a syringe were REALLY fucked there'd be millions of bacteria and no chance for any of the inoculations to work.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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Invisiblelone_psychonaut
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Re: Injector port location [Re: Inocuole]
    #23374841 - 06/23/16 07:00 PM (7 years, 7 months ago)

That makes sense. I'll begin researching agar; I really appreciate your help guys


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OfflineFreeWorldOrder
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Re: Injector port location [Re: lone_psychonaut]
    #23377152 - 06/24/16 11:29 AM (7 years, 7 months ago)

Good idea... agar is THE way to go... :headbang:


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"They who can give up essential liberty, to obtain a little temporary safety, deserve neither liberty nor safety." - Benjamin Franklin

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