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Offlineesse_jeremy
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What we have here???
    #23368039 - 06/21/16 07:37 PM (7 years, 8 months ago)

Hi all, I inoculated  5 little jars of agar with a print of cubensins and something grows and I make a transfert from one of this.

This is what I have



this is the one from isolates



now can you tell me what is the myc?
I think in the first picture u can see a myc but I'm not sure of the others
it's so difficult to compare with the white mold


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things does not exist,
everything is a process, so we proceed

Edited by esse_jeremy (06/21/16 07:57 PM)

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OfflineKenetic
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Re: What we have here??? [Re: esse_jeremy]
    #23368058 - 06/21/16 07:42 PM (7 years, 8 months ago)

What did you inoculate with? And I don't really understand your question.


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Invisibledankington
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Re: What we have here??? [Re: esse_jeremy]
    #23368568 - 06/21/16 10:42 PM (7 years, 8 months ago)

Quote:

esse_jeremy said:
...this is the one from isolates...




Typically, if you have an isolate, you've already done a substantial amount of cleaning the culture up. In other words, you wouldn't be asking if it were contaminated.

Perhaps I don't understand the question though.

The first pics definitely show a culture that has some bacteria within, as you can see by the bumps in the mycelial growth.

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Offlineesse_jeremy
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Re: What we have here??? [Re: dankington]
    #23369261 - 06/22/16 07:52 AM (7 years, 8 months ago)

okk and how can I isolate from the first plate??? I mean how to pick up only the myc??


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things does not exist,
everything is a process, so we proceed

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Invisibledankington
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Re: What we have here??? [Re: esse_jeremy]
    #23369265 - 06/22/16 07:57 AM (7 years, 8 months ago)

You can't from the first plate. An isolate means you have only isolated genetics in the sample. That usually happens after several transfers--at least 8 or so.

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Offlineesse_jeremy
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Re: What we have here??? [Re: dankington]
    #23369296 - 06/22/16 08:16 AM (7 years, 8 months ago)

right, I confused isolates with transfers sorry :frown:(

So I have transfer from the first plate to the last one...but also the transfer got a contam....my question is: how can I make a good transfer from the first plate?? (wich part I have to transfer ecc...)

Or I have to start over?


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Invisibleblindingleaf
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Re: What we have here??? [Re: esse_jeremy]
    #23369326 - 06/22/16 08:29 AM (7 years, 8 months ago)

i can barely make anything out, but i'd transfer from the first one, and then since ur tossing it anyway, take picture of it with the lid of the container off.

  it does kinda look thick like bacteria, but might be ur recipe(too heavy on the nutrients accidentally), or just early MS can also look shady/thick/strange.

the color in the center of ur second 3 pictures is tan, so i think thats weird.

the last pictures are just too jumbled and blurry…looks more like germination than a transfer.


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Offlineesse_jeremy
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Re: What we have here??? [Re: blindingleaf]
    #23369397 - 06/22/16 09:02 AM (7 years, 8 months ago)

The strange colour today turned into green so in the second 3 pics we got a contam dammm.

the last one that appear like a germination is the transfer of the first one....so if u want to see a transf of the first.....that is the result......

anyway I'm going to start over but first I have 2 sterelized empty pastry (just PDA agar) and I'm going to innoc. them, any advice? I got a ghetto SAB but I think that is not a good SAB.....how can I be more sterile when I innoc.??

(I use a loop burned to red, cool it down, then take spore from print and swipe plate)

It's my 3rd attempt and I had only one germination :frown: what a noob I am


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things does not exist,
everything is a process, so we proceed

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