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Offlinejivangilad
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G2G with only few grains?
    #23366822 - 06/21/16 01:24 PM (7 years, 7 months ago)

I do Rye grain jars, 1 quart.
Do G2G from a colonized rye grain jar.
Most teks say in G2G you need to pour 10 percent of the jar in grains.
Can I pour only like 2 tea spoons?
Will shaking help to spread the mycelium?
Or maybe shaking after a few days?
If I shake more vigorously, does it help to break mycelium from grains, and spread it?


Edited by jivangilad (06/21/16 01:26 PM)


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OfflineKenetic
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Re: G2G with only few grains? [Re: jivangilad]
    #23366847 - 06/21/16 01:38 PM (7 years, 7 months ago)

You can!  You could use one tea spoon even.  Shake the jar right after inoculation (with grain) and one more time at around 30-40℅.


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OfflineFreeWorldOrder
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Re: G2G with only few grains? [Re: jivangilad]
    #23366872 - 06/21/16 01:48 PM (7 years, 7 months ago)

A couple teaspoons will do fine...

Once you add the grains just shake enough to cover them.

Then shake again at 30% or so.

I will often give a final shake after full colonization to see how fast they recover.

If they do not recover in 2-3 days then I suspect bacteria and spawn those jars to an outdoor bed or separate mono or tray... :thumbup:


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OfflineKenetic
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Re: G2G with only few grains? [Re: FreeWorldOrder]
    #23366889 - 06/21/16 01:54 PM (7 years, 7 months ago)

Sometimes I shake my jars at full colonization if I can't spawn for a few days, buys me some time.  I don't like to let spawn consolidate. 
I've rarely do it to check for contams or recovery time but I can definitely agree with the logic behind it.


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Re: G2G with only few grains? [Re: Kenetic]
    #23366908 - 06/21/16 02:01 PM (7 years, 7 months ago)

I found 2 tsp works incredibly well if you use grass seed as your master jar.


It fits nicely between the larger grains and can be agitated, like a washing machine's motion, to filter it further down in the receiving jars


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OfflineMushroomBilly
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Re: G2G with only few grains? [Re: Snazz]
    #23366990 - 06/21/16 02:26 PM (7 years, 7 months ago)

One colonised grain is enough


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Re: G2G with only few grains? [Re: MushroomBilly]
    #23367002 - 06/21/16 02:30 PM (7 years, 7 months ago)

Eh but if you use more and mix it evenly right after the transfer, you can have full colonization in less than a week easily.


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Offlinespacechildo
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Re: G2G with only few grains? [Re: Inocuole] * 1
    #23367045 - 06/21/16 02:46 PM (7 years, 7 months ago)

and most likely have already done a 2nd round of g2g in the same time it took to colonize those first jars.


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Re: G2G with only few grains? [Re: spacechildo]
    #23367077 - 06/21/16 03:00 PM (7 years, 7 months ago)

Obviously more grains will make for faster colinization times. But if you needed to stretch your spawn you could easily turn one jar in 20 and.more. I make one master jar into 14 as my pressure cooker holds 14 quart jars. Ive had to spread one jar thinner and further in the past. It will just take longer but yea. Technically one colonized grain or peice of grain introduced in sterile conditions to sterilized spawn would make tons of jars.


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Offlinespacechildo
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Re: G2G with only few grains? [Re: spacechildo]
    #23367086 - 06/21/16 03:02 PM (7 years, 7 months ago)

my point was it would go faster in 2 rounds with more spawn to each jar.
you'd also end up with way more jars, if that was your original concern.


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OfflineKenetic
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Re: G2G with only few grains? [Re: DeadPhan]
    #23367089 - 06/21/16 03:03 PM (7 years, 7 months ago)

I agree.  Do what you have to with your resources.


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OfflineDeadPhan
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Re: G2G with only few grains? [Re: DeadPhan]
    #23367095 - 06/21/16 03:05 PM (7 years, 7 months ago)

Also, I used to shake right after a g2g but I have since left the grains to sit at the top for the first 2 to 3 days so as they recover and then shake em up. I cant say ive noticed a huge difference but, I like to do it as a contam barrier at first. Where as the colonized grain layer on top might be less susceptible. Inly started doing that recently tho.


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OfflineKenetic
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Re: G2G with only few grains? [Re: DeadPhan]
    #23367104 - 06/21/16 03:08 PM (7 years, 7 months ago)

Quote:

DeadPhan said:
Also, I used to shake right after a g2g but I have since left the grains to sit at the top for the first 2 to 3 days so as they recover and then shake em up. I cant say ive noticed a huge difference but, I like to do it as a contam barrier at first. Where as the colonized grain layer on top might be less susceptible. Inly started doing that recently tho.



Hmmm.  Aren't you disturbing them twice then?


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Offlinekyu
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Re: G2G with only few grains? [Re: MushroomBilly]
    #23367155 - 06/21/16 03:21 PM (7 years, 7 months ago)

Quote:

MushroomBilly said:
One colonised grain is enough




I used one grain and it worked just fine. 10%-rule is simply faster.


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OfflineHybridprX
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Re: G2G with only few grains? [Re: kyu]
    #23367308 - 06/21/16 04:04 PM (7 years, 7 months ago)

1 will work...

4 will finish a jar in 7 days...


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Offlinejivangilad
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Re: G2G with only few grains? [Re: HybridprX]
    #23368573 - 06/21/16 10:46 PM (7 years, 7 months ago)

Quote:

HybridprX said:
1 will work...

4 will finish a jar in 7 days...



Do you need to shake jar vigorously so that mycelium breaks and spread?
The reason I asked the question, is that I used 2 teaspoons.
I did shake them after inoculation.
after 10 days, it is still very far from full colonization.
Maybe there are just like 6-8 times more colonized grains then in the beginning.
I have though ATL#7 species, maybe a bit slower colonizer, but shouldn't be that much slower.
Everybody seems to get such a quick colonization...


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InvisibleSnazz
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Re: G2G with only few grains? [Re: jivangilad]
    #23368613 - 06/21/16 11:02 PM (7 years, 7 months ago)

The trick is:  it needs to be very well distributed.  So each kernel only has to colonize 1.5" in any direction.


Hull-on grains recover faster too, as there is more myc inside than outside, so less damage.


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OfflineHybridprX
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Re: G2G with only few grains? [Re: Snazz]
    #23369058 - 06/22/16 05:09 AM (7 years, 7 months ago)

Quote:

Snazz said:
The trick is:  it needs to be very well distributed.  So each kernel only has to colonize 1.5" in any direction.


Hull-on grains recover faster too, as there is more myc inside than outside, so less damage.




The more distributed you get the mycelium the faster it will colonize, yes. When using 1 to 2 tps you will need to shake the jar again around 50% to redistribute colonized grain for optimum times...

If you use 4-6 you only should have to shake it to distribute the original grains.


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