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OfflineWill7401
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Registered: 01/27/16
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Re: All white not always all good [Re: SpitballJedi]
    #23366344 - 06/21/16 09:47 AM (7 years, 7 months ago)

I originally started with MS with no success. I quickly learned the Pasty Plate agar tek and have been using that ever since. It was suggested that I go 2 hours for PC instead of 90 so I started doing that as well but not these jars. Also, I was using tow layers of micropore tape as a filter but have now switched to tyvek. These jars had the tape. My technique is improving over time. I stay open minded to any suggestion.


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InvisibleSpitballJedi
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Re: All white not always all good [Re: mupetmower]
    #23366359 - 06/21/16 09:52 AM (7 years, 7 months ago)

Quote:

mupetmower said:
wait, why if youre a guy you might see green when actually blue? just curious haha. never heard that guys and girls see colors differently, or that one can have partial color blindness or something?




Men are statistically more prone to being color-blind or see shades differently. My step dad couldn't see blue and he saw red and green as the same.

Apparently that's not the OP's issue.


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InvisibleSpitballJedi
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Re: All white not always all good [Re: Myko Fanatikos]
    #23366371 - 06/21/16 09:55 AM (7 years, 7 months ago)

Quote:

Myko Fanatikos said:
I PC for 50 minutes to an hour.





A minimum of 90 mins at 15psi is recommended. I've started doing 120 mins


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InvisibleSpitballJedi
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Re: All white not always all good [Re: Will7401]
    #23366395 - 06/21/16 10:02 AM (7 years, 7 months ago)

Quote:

Will7401 said:
I originally started with MS with no success. I quickly learned the Pasty Plate agar tek and have been using that ever since. It was suggested that I go 2 hours for PC instead of 90 so I started doing that as well but not these jars. Also, I was using tow layers of micropore tape as a filter but have now switched to tyvek. These jars had the tape. My technique is improving over time. I stay open minded to any suggestion.




Yes, micropore tape sucks.

You may not be using clean culture. You have to get better at identifying contams on agar and transferring away from it. You also need to make sure you aren't contaminating the agar while working. Avoid moving your hands over open sterile media and do your work toward the back of your SAB.

As I said earlier, if you have green mold in your jar, toss it. No good will come of it.


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Offlinespacechildo
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Re: All white not always all good [Re: SpitballJedi]
    #23366408 - 06/21/16 10:06 AM (7 years, 7 months ago)

This may come out wrong but I'm struggling to believe any of these jars showed green mold 1 hr after shaking up,
left one's got a fair bit bacteria, sure, but still not mold. and green is usually the molds spores which I cant understand how it got to in a grain jar that quickly.


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InvisibleSpitballJedi
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Re: All white not always all good [Re: spacechildo]
    #23366447 - 06/21/16 10:19 AM (7 years, 7 months ago)

That's why I kept pressing about the color. An hour is pretty damn fast for mold, IME. Even when I see mold on something already, it just doesn't grow that fast.


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Invisiblenatedawgnow
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Re: All white not always all good [Re: spacechildo]
    #23366454 - 06/21/16 10:20 AM (7 years, 7 months ago)

The mold could easily have sporelated in the middle of the jar and it was
not visible until it was shaken up and op just didn't notice at 1st glance
after shaking. I have seen that before for sure.


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OfflineMycoLoopology
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Re: All white not always all good [Re: natedawgnow]
    #23366542 - 06/21/16 10:58 AM (7 years, 7 months ago)

im literally just chiming in here because someone mentioned smacking the jars using your hand. not that he/she was advocating for it, but i wanted to add that i once had a jar with myc that didnt want to break up(definitely bacterial contam in that fucker of a jar)...

anyway i got a bit angry and i started slamming my palm/hand all over the jar; and while the myc still hadnt broken up, the glass jar HAD. all over my hand and arm.. lots of cuts, lots of blood; still have the scars.

use a tire. if it doesnt break up on a tire? its probably got bacterial contam. or ive noticed if i let jars sit too long they get harder to break up as well. but either way, dont get angry and use your hand :p i learned my lesson the hard way.


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Invisibledankington
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Re: All white not always all good [Re: MycoLoopology]
    #23366572 - 06/21/16 11:10 AM (7 years, 7 months ago)

If it doesn't break against your palm, it's probably bacterial. If you start really going to town, don't use your hand. :shrug:

Only jar that has broken in my hand was one I was shaking up too soon after PC'ing. Luckily I was holding it by the lid.


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InvisibleSnazz
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Re: All white not always all good [Re: dankington]
    #23366591 - 06/21/16 11:19 AM (7 years, 7 months ago)

If it doesn't bust up, just scoop/flake it out.  Or let it consolidate and shrink a bit,  then skewer it and pull.


I don't like the idea of beating jars,  or bruising / cutting myself much


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InvisibleMad Season
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Re: All white not always all good [Re: spacechildo]
    #23366742 - 06/21/16 12:30 PM (7 years, 7 months ago)

Quote:

spacechildo said:
Quote:

Myko Fanatikos said:
I don't understand how you can tell it's bacterial from looking?

All my jars have smelled like straight mushroom, no sweetness, nothing. I PC for 50 minutes to an hour.

All my tubs have given me 4 flushes as well. If they were bacterial would they still produce for so long without a legit contam?




you can see its bacterial in the way its trying to recolonize the millet on the bottom of the pic but rather grows fuzz around it than colonize it.
If it was healthy it wouldn't look like that after 48hrs.

also 50mins PC time is pushing it, 90-120mins @15psi drasticly reduces bacteria colonies.

bacteria doesnt always mean you'll get contams, it just means you myc is spending a lot of energy fighting the bac instead of focusing on producing mushrooms.
If you get 4 good flushes you should try and better the fruiting conditions, more FAE would be the first thing I'd look into.



:whathesaid: also once you get a huge trich outbreak, let's see you get 4 flushes with that POS spawn jar. I used to open air g2g bacterial af jars thinking they were clean and got a couple flushes out of them. Now that trich has been unleashed in my house, I don't even bother with jars that make me even the slightest concerned.


Also.. yeah myc can bruise green.

that is bruising and it has no blue to it.


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Edited by Mad Season (06/21/16 12:39 PM)


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OfflineMyko Fanatikos
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Re: All white not always all good [Re: Mad Season]
    #23366818 - 06/21/16 01:22 PM (7 years, 7 months ago)

Lol I'll cross the green bridge of trich when I get to it. With 4 cats and a dogs worth of hair floating around my house I'm surprised I haven't gotten more than cobweb so far :tongue2:

If I see anything white that even looks close to trich I'll have that tub sitting outside in a heartbeat.

One of my first tubs looked suspect and it went straight to the back porch.

Thanks for the info guys, without all you I wouldn't know my ass from a hole in the ground lol


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OfflineKenetic
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Re: All white not always all good [Re: Myko Fanatikos]
    #23366838 - 06/21/16 01:34 PM (7 years, 7 months ago)

Quote:

Myko Fanatikos said:
I wouldn't know my ass from a hole in the ground lol




You gave me that impression lol.  Keep trying, you'll figure out what works for you eventually.


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OfflinePerception7
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Re: All white not always all good [Re: Kenetic]
    #23366927 - 06/21/16 02:06 PM (7 years, 7 months ago)

Concerning how mycelium should look, I am FAR from an expert. Almost every time someone says a jar looks bacterial I cannot see anything wrong with the jar. I always spawn jars if they are white with no discoloration, and smell good when opening. Jars break up easily in my hand as well... well usually, except for that one time I had one bust on me and cause some blood to spill.

OH and I collected the mycelium that busted all over the dirty carpet, blood and all, put it inside a pan and fruited it, and got lots of mushrooms :lol:

The pic of the pan below was made with 1 quart of mycelium on rye grain, the other 4 jars I had contaminated with trich.

The pan got bacterial though, never contaminated however (flushed 4-6 times before I chucked it) here is a pic, you can see the white balls of bacteria on the surface, still got many ounces of dried mushrooms... :lmafo:

My blood and sweat have literally been grown into this hobby.



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Edited by Perception7 (06/21/16 02:12 PM)


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OfflineMyko Fanatikos
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Re: All white not always all good [Re: Kenetic]
    #23366945 - 06/21/16 02:13 PM (7 years, 7 months ago)

Quote:

kenetic said:
Quote:

Myko Fanatikos said:
I wouldn't know my ass from a hole in the ground lol




You gave me that impression lol.  Keep trying, you'll figure out what works for you eventually.




Lmao, one day I'll get my shit together. It never hurts to be humbled once in a while.

I'm grateful for the advice :thumbup:


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OfflineKenetic
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Re: All white not always all good [Re: Perception7]
    #23366968 - 06/21/16 02:19 PM (7 years, 7 months ago)

Quote:

Perception7 said:



My blood and sweat have literally been grown into this hobby.






Ha that's great!  Waste not lol

Oh and myco I meant no offense.  I have a ton to learn as well and I couldn't have done anything without this site.  I'm no scientist either lol


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OfflineMyko Fanatikos
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Re: All white not always all good [Re: Kenetic] * 1
    #23367160 - 06/21/16 03:22 PM (7 years, 7 months ago)






Oh and myco I meant no offense.  I have a ton to learn as well and I couldn't have done anything without this site.  I'm no scientist either lol




If I took offense instead of advice I'd never get anywhere :wink:


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OfflineWill7401
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Re: All white not always all good [Re: Myko Fanatikos]
    #23370953 - 06/22/16 07:08 PM (7 years, 7 months ago)

Hey everyone. I'm posting pics that were taken today after a attempted to bang up a few more jars of the same group as before. First off, let me just say that after two days, the original jars have recolonized bright white and are no longer showing any green. The first jar is before shaking. The rest are after. They Are not all the same jar but from the same group. They all looked colonized the same as the first one so I didn't waste time posting all of them. The rest are about 30 mins after shaking. I am still relatively new to mycology so I do not have experience at every level, hence the motive for this post: the experience of others. If this is blue bruising, I will take it. If it's not, anyone seen this before. I've never seen this in any of my jars before.   [image]http://www.shroomery.org/forums/thumbs/16-25/664334428-thumb_WP_20160622_18_27_31_Pro.jpg[/image]


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OfflineKenetic
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Re: All white not always all good [Re: Will7401]
    #23370992 - 06/22/16 07:18 PM (7 years, 7 months ago)

After the shake?  They must have been really consolidated to start bruising like that.  I can't tell for sure but I think its all right.


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Invisibledankington
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Re: All white not always all good [Re: Kenetic]
    #23371043 - 06/22/16 07:30 PM (7 years, 7 months ago)

Those look awfully sketchy to me.


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