Home | Community | Message Board

MRCA Tyroler Gluckspilze
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Kraken Kratom Red Vein Kratom

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinelethalbacon
Stranger

Registered: 01/12/16
Posts: 9
Last seen: 1 year, 7 months
Are these LC jars OK to use?
    #23361392 - 06/19/16 05:09 PM (7 years, 7 months ago)

Hey all,

So, I recently started a few jars of LC from a spore print from my last harvest. Figured it'd be easier and faster than using spore syringes. I thought that they were good to use, as they certainly seemed like they were on the right track.

A few days ago, I inoculated eight BRF cakes, noting which jars came from which LC so that I could track any contamination. Unfortunately, five of my eight jars are definitely molding, and I have suspicions about the other three. I've never lost more than one jar out of eight, and so I'm fairly confident that my procedure is at least fairly sterile. Thus, I'd appreciate any feedback that you guys could give on my LC jars. My biggest concern is that I didn't wait long enough for the LC jars to fully colonize. Well, I pretty much know that I didn't, but I figured that as long as there was mycelium present in the syringe that it would be effective.

On an unrelated note, any ideas on why my cakes always flush at different times? I always see these beautiful pictures of SGFCs with a sea of mushrooms on every cake, but I've never had more than one or two cakes flush at a time.

Thanks as always!





Extras: Filter Print Post Top
OfflineMycoLoopology
Eye's wide open
 User Gallery


Registered: 06/12/16
Posts: 518
Last seen: 2 years, 7 months
Re: Are these LC jars OK to use? [Re: lethalbacon]
    #23361399 - 06/19/16 05:12 PM (7 years, 7 months ago)

Quote:

lethalbacon said:
Hey all,

So, I recently started a few jars of LC from a spore print from my last harvest. Figured it'd be easier and faster than using spore syringes. I thought that they were good to use, as they certainly seemed like they were on the right track.

A few days ago, I inoculated eight BRF cakes, noting which jars came from which LC so that I could track any contamination. Unfortunately, five of my eight jars are definitely molding, and I have suspicions about the other three. I've never lost more than one jar out of eight, and so I'm fairly confident that my procedure is at least fairly sterile. Thus, I'd appreciate any feedback that you guys could give on my LC jars. My biggest concern is that I didn't wait long enough for the LC jars to fully colonize. Well, I pretty much know that I didn't, but I figured that as long as there was mycelium present in the syringe that it would be effective.

On an unrelated note, any ideas on why my cakes always flush at different times? I always see these beautiful pictures of SGFCs with a sea of mushrooms on every cake, but I've never had more than one or two cakes flush at a time.

Thanks as always!








i have no idea on the LC's so someone else can chime in on that. but as for why you only get mushrooms on one cake and not another, imo, is based on where the best micro-climate is being formed in your chamber. id bet that if you moved some of your non fruiting cakes into the area where your fruiting cakes are, youll find that they start fruiting faster while the others start going slower.

or i could be completely wrong because i have no idea what your set-up looks like :p good luck though bud!


--------------------
Current Grow Log

^^My first grow log <3^^

Wish me luck.


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Are these LC jars OK to use? [Re: MycoLoopology]
    #23361414 - 06/19/16 05:17 PM (7 years, 7 months ago)

There is no way to visually tell if a LC is clean, that is why it's recommended as an advanced technique.  It has to be tested before you can use it to inoculate any large amount of anything, and even then, with poor sterile technique, the entire batch could become contaminated while drawing liquid from the jar, so you could, at any time, experience a large amount of failure.  Cleaning out a bunch of jars sucks.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
OfflineBobabouy
Shrimp Boat Captain
Male


Registered: 01/19/16
Posts: 661
Loc: The Black Sea
Last seen: 6 years, 16 days
Re: Are these LC jars OK to use? [Re: Inocuole]
    #23361422 - 06/19/16 05:19 PM (7 years, 7 months ago)

Quote:

Inocuole said:
There is no way to visually tell if a LC is clean, that is why it's recommended as an advanced technique.  It has to be tested before you can use it to inoculate any large amount of anything, and even then, with poor sterile technique, the entire batch could become contaminated while drawing liquid from the jar, so you could, at any time, experience a large amount of failure.  Cleaning out a bunch of jars sucks.




Extras: Filter Print Post Top
Offlinelethalbacon
Stranger

Registered: 01/12/16
Posts: 9
Last seen: 1 year, 7 months
Re: Are these LC jars OK to use? [Re: Bobabouy]
    #23361473 - 06/19/16 05:37 PM (7 years, 7 months ago)

Yeah, kinda what I figured. I'll give it one more shot and then go back to spore syringes, I suppose.

Thanks!


Extras: Filter Print Post Top
InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
 User Gallery


Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: Are these LC jars OK to use? [Re: lethalbacon]
    #23361478 - 06/19/16 05:39 PM (7 years, 7 months ago)

Quote:

lethalbacon said:
Yeah, kinda what I figured. I'll give it one more shot and then go back to learn how to do agar from spore syringes, I suppose.

Thanks!




Fixed it for you homie...:seriousthumbsup:


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


Extras: Filter Print Post Top
OfflinePsilosoulful

Registered: 09/05/14
Posts: 7,205
Last seen: 1 year, 1 month
Re: Are these LC jars OK to use? [Re: LocN9ne]
    #23361530 - 06/19/16 05:59 PM (7 years, 7 months ago)

Agar :heart:


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Kraken Kratom Red Vein Kratom


Similar ThreadsPosterViewsRepliesLast post
* Is it a good idea to create jars way b4 inculation rain_angel 1,664 11 05/27/02 04:44 PM
by Dex_Detrimental
* Could this lead to contamination??? roykinn 671 2 01/26/02 12:33 AM
by roykinn
* H2O2 use, and multiple strains in one jar pulserate 910 3 09/01/01 01:06 AM
by Zen Peddler
* Jars getting air Upsidedown lid PuffinSmoke 1,145 3 06/04/02 06:10 PM
by PuffinSmoke
* Cracked Mason Jars / Lysol Question friendlyfriends 2,062 11 08/24/02 08:40 PM
by Aeolus1369
* Need help--Should I use contaminated jar??? Eyeball_Kid3 843 3 06/27/02 03:35 PM
by goolag
* Re: First grow- jar went invitro on accident JeeLiX 1,514 2 04/03/01 11:23 PM
by JeeLiX
* Re: contaminated Jars paddoholland 977 4 03/15/01 11:06 PM
by Shroomzilla

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
207 topic views. 28 members, 234 guests and 41 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.01 seconds on 14 queries.