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mothballs
Stranger in a strange land



Registered: 04/30/10
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albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms)
#23356841 - 06/18/16 05:33 AM (7 years, 7 months ago) |
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lets start w the article: then the souces and laymans terms.....
method for the production of 7.5 grams of psilocybin in less than a week and a half.
Typical Ps.C. cultures grown by various organic substrates achieve .4 - .6 alkaloid yields. Typical nutrient enhanced substrates achieve slightly higher yields of alkaloids. Liquid cultures can effectively achieve 1 - 1.1 alkaloid yields by making essential nutrients and carbohydrates easily available.
This is the general potency of this strain; attempts to further improve nutrient uptake will most likely only result in a higher biomass yield. In the instance of the introduction of tryptamine to yield 3% psilocin alkaloid, I view it as a novel biosynthesis mechanism, operating externally of the natural model of the organism.
Total biomass, although affected accumulatively, is proportional to carbohydrate uptake. I have heard unofficial reports of rapid growth with dextrose as well as organic honey, which is composed of multiple complex sugars. Perhaps a more efficient mixture of carbohydrates then just glucose could be developed.
Many funguses grow rapidly in an acidic environment (3 - 5 pH). Ours displays higher yields of alkaloids and biomass between 4 - 4.6 pH but loses durability and suffers from tissue damage and possible cellular ruption due to solution agitation during the process of aeration. I assume that the growth acceleration that occurs at a lower pH is because nutrients are able to permeate the cellular equilibrium easier. Taking advantage of this requires a device capable of aerating the solution without disturbing the mycelium.
This method of mycelium harvest is far superior to fruited bodies in relation to alkaloid yields, processing, extraction, and overall time.
Lets Pretend:
Glucose (C6H12O6) [1000 g] Ammonium Succinate (NH4OOC-CH2-CH2-COO) [200 g] Yeast Extract (Organic Compound) [100 g] Magnesium Sulfate (MgSO4-7H2O) [100 g] Potassium Phosphate (KH2PO4) [20 g] Thiamine Hydrochloride (C12H17ClN4OS HCl) [600 mg] Ferrous Sulfate (FeSO4-7H2O) [500 mg] Cupric Sulfate (CuSO4-5H2O) [100 mg] Ammonium Heptamolybdate ((NH4)6Mo7O24-4H2O) [10 mg] Manganese Chloride (MnCl2-4H2O) [7 mg] Zinc Sulfate (ZnSO4-7H2O) [6 mg] Water (H2O) [200 L]
Prepared in 55 Gallon (211L) drum and adjust to pH 5.5 with hydrochloric acid.
a. Lid is sealed and capped with a filtered pressure release valve. b. Drum and solution is heat sterilized. c. Cap is swabbed with sterile gauze and H2O2 and removed. d. Solution inoculated with 1L of precultured Mycelium. e. Insertion aerator and cap with filtered relief check valve. f. Solution is kept at 30C with an electric blanket. g. Solution is aerated for 7 days with filtered air. h. 235 Ounces (14.5 lbs) of mycelium are strained from solution with cloth i. Mycelium dried over calcium chloride to yield 750g dry weight. j. Biomass is ground to a fine powder and re-added to drum with 75 L of methanol. k. Sealed with cap fitted with large egg whisk style blender attached to the bottom. l. Heated to 40C with electric blanket for 1-4 hours depending on level of agitation. m. Solution is filtered with cloth. n. Solution is re-filtered in a funnel with inert filtration medium. o. Cap is fitted with a condenser (.5 - 1" coiled copper tubing inside 4-5 ft 6" PVC pipe allowing for coolant flow around coil) and digital thermometer to measure solution temp. p. Sealed heat element is placed under drum to maintain 65C. q. Solution is distilled to 1-5 liters. r. Solution transferred to distillation apparatus and distilled in 1 L batches. s. Yielding 20-40 grams of residue. t. Residue is developed in a 100 ml H2O and 500 ml Naptha matrix. u. Solution is agitated for 1 hour at room temperature. v. Non-polar proteins and oils are removed via sep funnel separation of naptha layer. x. Water is evaporated from extract with a blower using calcium chloride at 30C in 2 hours. y. Yielding 10-20 grams of extract containing 7.5 g of psilocybin. z. 375 doses with a street value of over $7000.00
I digress:
If boiling 75 L of methanol isn't your idea of time saving, cost efficient or fun, you can opt to process your ground-dried biomass in 75 (10 g / 1 L) batches. The major benefit being that you can reclaim most your solvent, eliminating the need to buy bulk methanol. The downside is the time involved, even under vacuum this is going to take a little while. Under optimum conditions we could still complete the entire process in less than two weeks.
Perhaps a reflux percolator could be implemented to percolate the bulk material with 1 L methanol while collecting extracts in the distillation chamber. (i.e. the methanol condenses from the distillation chamber into a reservoir layered with: ground biomass and perculate, inert filtrate medium, and filter material; then drains back into the distillation chamber.) Percolation should be complete within 4-24 hours. Fastest results would be seen if condenser displaced about 20C. Alternatively the reservoir could act as a condenser with the addition of a cooling coil to displace vapor temperature and instigate methanol condensation while passing thru the biomass perculate. Biomass should be free of alkaloids upon completion and can be tested for their presence using Keller's reagent. Remaining methanol is distilled off.
Under optimum conditions the entire process could take less than a week and a half.
-Zen
Ref: Hoffmann, Albert "Obtaining Psilocybin and Psilocin from Fungal Material" Sandoz Ltd., US patents 3183172, 1959
Ref: Hofmann, Albert "Method of Inducing Therapeutic Tranquilization with Psilocybin and Psilocin" Sandoz Ltd., US patents 3192111, 1959
Ref: Catalfomo, P. and V.E. Tyler, Jr. "The Production of Psilocybin in Submerged Culture of Psilocybe Cubensis" LLoydia 27:53-63, 1964
Ref: Gartz, Jochen "Synthetic Nutrient Medium for Fungal Manufacture of Indole Alkaloids" Ger. (East) DD 255,749 (cl. C12P15/00), 13 Apr 1988
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: mothballs]
#23356843 - 06/18/16 05:34 AM (7 years, 7 months ago) |
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Disproved time and time over
Genetics dictate potency.
More potent species grow on less nutritional substrate. Likewise cubes grown on overly nutritional substrate like cakes do not make more potent mushrooms. Likewise an isolate grown on any substrate is just as potent as it is grown on any other.
Edited by Trusted cuItivator (06/18/16 05:36 AM)
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mothballs
Stranger in a strange land



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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: bodhisatta]
#23356844 - 06/18/16 05:35 AM (7 years, 7 months ago) |
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so, what does that say????? 100 g corn sugar 200g succinic acid (ebay) 20 g potasium phosphate. (americanspice.com) 100g epsom salt 100g yeast extract 600mg vitamin B1 500mg green vitroile (naturalpigment.com) 100mg blue vitriole (amazon) 10mg amonium molybendate(ebay) 7mg manganeese chloride (ebay) 6mg white vitrole (naturalpigments.com) 100L water
this will make an lc specifically designed by albert hoffman to make a particulary potent mycelium and is most likely the most scientific data out there on the nutes required for potency. as far as the extraction goes,.... 75 l of meoh for >1 kilo of dryed matter.....?????? WTF, mr.hoffman, phytochemistry wasnt your strong suit. put it on a meoh reflux in a 3L soxholet system to yeild perfect crystals lickity split!
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mothballs
Stranger in a strange land



Registered: 04/30/10
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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: bodhisatta]
#23356847 - 06/18/16 05:37 AM (7 years, 7 months ago) |
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refrences please, oh, ps, he pattented it
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: mothballs]
#23356850 - 06/18/16 05:39 AM (7 years, 7 months ago) |
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Patents don't mean shit 
The vast majority of patents are crap, BS, useless, etc..
50+ years of no one giving a shit should be enough reference for anyone with 4th graders research skills
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mothballs
Stranger in a strange land



Registered: 04/30/10
Posts: 242
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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: bodhisatta]
#23356855 - 06/18/16 05:41 AM (7 years, 7 months ago) |
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we are talking about a. hoffman here. his phytochem skills were questionable, but.....
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: mothballs]
#23356859 - 06/18/16 05:44 AM (7 years, 7 months ago) |
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I know who Hoffman is.
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mothballs
Stranger in a strange land



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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: mothballs]
#23356862 - 06/18/16 05:45 AM (7 years, 7 months ago) |
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so, do you think it wont work??? or it just wont increase potency??.. or would it still works as a complex lc..... and could you eat dryed myc instead of friut bodys?
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: mothballs]
#23356866 - 06/18/16 05:50 AM (7 years, 7 months ago) |
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I think the media recipe will work, no better or worse than grain water or malt extract.
Your time would be 1000x better spent doing agar isolating to get a more potent set of genetics that grows a higher % alkaloid content every time regardless of what it's grown on as long as it is grown on something acceptable for the species
For the complexity of the LC (even if) it magically made a difference it would still be much more efficient to just use say 5% more mushrooms/mycelium grown on much more simple substrate/media
Eating dried mycelium is noob shit
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: mothballs]
#23356868 - 06/18/16 05:52 AM (7 years, 7 months ago) |
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Why does someone always come in here about once a month and quote some incredibly old and outdated article and call it superior just because it's "referenced"?
Hoffman was a genius, there is no refuting that but so was Thomas Edison, do we still use DC as a main electrical supply? No, we don't. That's because something that was published decades ago is usually improved on, effectively making it outdated.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: Supalemonhaze]
#23356870 - 06/18/16 05:54 AM (7 years, 7 months ago) |
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But there's patents on DC transmission, PATENT!
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
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Loc: 12" down Europe's butthole
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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: bodhisatta]
#23356875 - 06/18/16 05:58 AM (7 years, 7 months ago) |
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Oh, my bad. I should probably tell the government that they are doing it all wrong, after all, the patents are legit.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: Supalemonhaze]
#23356881 - 06/18/16 06:03 AM (7 years, 7 months ago) |
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They actually do use DC for transmission lines across huge distance and because not every AC grid is compatible they have individual AC/DC conversion at localities. But that wasn't Edison stuff anyway
Hoffman was the guy who called mushrooms plants so...
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mothballs
Stranger in a strange land



Registered: 04/30/10
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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: bodhisatta]
#23356884 - 06/18/16 06:04 AM (7 years, 7 months ago) |
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lol, eating myc full of casing, verm, shit, and god knows what else would be some dumb shit to do,... but if it were cultivated in a clean lc? it Does contain the magic
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: mothballs]
#23356893 - 06/18/16 06:07 AM (7 years, 7 months ago) |
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Probably very very little if any. When mushroom primordia form that's when the biological trigger to produce our alkaloids starts.
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mothballs
Stranger in a strange land



Registered: 04/30/10
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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: mothballs]
#23356898 - 06/18/16 06:09 AM (7 years, 7 months ago) |
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a misnomer for mushies, and accurate for the majority rest of the book( plants of the gods). probally his attempt at laymans terms
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Fractal420
Psycellium



Registered: 06/21/13
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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: mothballs]
#23356948 - 06/18/16 06:38 AM (7 years, 7 months ago) |
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Dont compare hofmann to edison.
Edison was a prick
It also mentions tryptamine hcl increasing psilocin to 3% (which is insane). These shrooms, you wouldnt even take a whole gram probably
Anyone out there wanna put some NN-MIPT in substrate??? Please??
-------------------- Dreaming of That face again. It's bright and blue and shimmering. Grinning wide And comforting me with it's three warm and wild eyes. Prying open MY third eye
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bodhisatta 
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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: Fractal420]
#23356960 - 06/18/16 06:43 AM (7 years, 7 months ago) |
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Tyrptamine is totally broke down by the mycelium it doesn't assimilate precursor building blocks. And that's how we can prove that even Hoffman Bullshits
People have tried putting all sorts of things in their substrate from mushrooms to DMT to tryptamine and none of it does shit
Besides that's as stupid as using really good weed as the soil for new weed
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blackout


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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: mothballs]
#23356970 - 06/18/16 06:48 AM (7 years, 7 months ago) |
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why did you not post the link to the original thread that you cut & pasted into the first post?
https://www.shroomery.org/forums/showflat.php/Number/514231
There was no mention of agar in that post, but in the Sandoz patents there is. One poster claimed to be key in making this work, and why Adam Gottlieb's instructions would not work in his guide. The soft agar uses 1/10th the usual amount people use, this creates a thick gel.
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Fractal420
Psycellium



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Re: albert hoffmans recipie for synthetic nutrients designed for potentcy( laymans terms) [Re: bodhisatta]
#23356981 - 06/18/16 06:53 AM (7 years, 7 months ago) |
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Quote:
Trusted Cultivator said: Tyrptamine is totally broke down by the mycelium it doesn't assimilate precursor building blocks. And that's how we can prove that even Hoffman Bullshits
People have tried putting all sorts of things in their substrate from mushrooms to DMT to tryptamine and none of it does shit
Besides that's as stupid as using really good weed as the soil for new weed
If it worked it wouldnt be stupid at all. Ive read about this in TIHKAL too. I am not sure if Shulgin was speaking from experience , but this is in TIHKAL:
Some fascinating studies have been done in Germany where the metabolically active mycelium of some Psilocybe species have been administered diethyltryptamine as a potential diet component. Normally, this mushroom species dutifully converts N,N-dimethyltryptamine (DMT) to psilocin, by introducing a 4-hydroxyl group into the molecule by something that is probably called an indole 4-hydroxylase by the biochemists. You put DMT in, and you get 4-hydroxy-DMT out, and this is psilocin. Maybe if you put Mickey Mouse in, you would get 4-hydroxy-Mickey Mouse out. It is as if the mushroom psyche didn't really care what it was working with, it was simply compelled to do its sacred duty to 4-hydroxylate any tryptamine it came across. It was observed that if you put N,N-diethyltryptamine (DET, not a material found in nature) into the growing process, the dutiful and ignorant enzymes would hydroxylate it to 4-hydroxy-N,N-diethyltryptamine (4-HO-DET) a potent drug also not known in nature. This is the title drug of this commentary. What a beautiful burr to thrust into the natural versus synthetic controversy. If a plant (a mushroom mycelium in this case) is given a man-made chemical, and this plant converts it, using its natural capabilities, into a product that had never before been known in nature, is that product natural? What is natural? This is the stuff of many long and pointless essays.
-------------------- Dreaming of That face again. It's bright and blue and shimmering. Grinning wide And comforting me with it's three warm and wild eyes. Prying open MY third eye
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