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ClintMassey
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Registered: 05/31/16
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A stupid question about colonization!
#23355279 - 06/17/16 04:05 PM (7 years, 7 months ago) |
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Sorry in advance but i have a large redboy spawn and most of my jars are halfway colonized the top half is fully colonized and the bottom half is still not,Do i really need to shake the grains in order to colonize it or will it make it eay trough eventually,its such a beautifull thick rhizomorfic mycelium that i really dont want to tear it apart by shaking and its so much dense that i dont believe I could even break it apart,only if i opened the jars and to it with a knife or something cause that shit is really monstrous dense and thick mycelium
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
Edited by ClintMassey (06/17/16 04:06 PM)
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



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Posts: 7,076
Loc: to the brain
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Re: A stupid question about colonization! [Re: ClintMassey]
#23355359 - 06/17/16 04:35 PM (7 years, 7 months ago) |
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You should shake them... Definitely do not open them.
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Q&A US vs. THEM The more I learn, the less I know.
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ComebackKid
Multispore Enthusiast



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Re: A stupid question about colonization! [Re: ClintMassey]
#23355555 - 06/17/16 05:53 PM (7 years, 7 months ago) |
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I wanna pic first before you shake em though
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Substrate surface conditions / Monotub prep and care
Look around you... Everything you see exists inside the mind. Consciousness, the awareness that is experiencing this mind, is peering in from outside the universe. Our individual experiences are all part of the universe's experience of itself
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bodhisatta 
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Re: A stupid question about colonization! [Re: ComebackKid]
#23355577 - 06/17/16 05:59 PM (7 years, 7 months ago) |
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thick rhizomorphic growth sounds like bacteria. pictures
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ClintMassey
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Re: A stupid question about colonization! [Re: bodhisatta]
#23355912 - 06/17/16 08:03 PM (7 years, 7 months ago) |
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No I dont think its bacterial infection its pure mycelium,smells like pure mushrooms and there are no type of slushines or usual marks of bacteria,smell and looks are normal,I didnt think of this as a sort of a problem its normaly filling up the jar...I just asked do I need to shake the jars or will mycelium colonize the jar itself so I dont need to break it all up apart and wait for it to grow back once again cause I really hate to do that
Here are the pictures only they are bad quality with a shitty mobile camera so you will see it a little blurry but I tried to focus on the rhizomorphic growth I usually get fuzzy and all other types of growth and rarely pure rhizomorphical so that s why I said thick rhizomorphic growth,its actually a mix of everything pure with and very nice buildup pretty fast colonizer on some jars,it just went straight trough and I use barley seeds so it usually starts on the upper half and always delays somehow on the lower part,maybe because of the moisture in the seeds or something I dont know




And here is the side where it didnt colonised properly yet,so that s why I asked do I need to shake and brake it or will it colonise itself,in the end I like it when its nice and thick and hard to break up cause I sterilize a spoon and then scrape it out into another big jar and mix it all up very good and then spawn into a bulk with that mix...that s what I did before and it worked great,mycelium doesnt get stunted like when you shake and break and it spawns better and faster

Thanks for the worries but here take a look and jugde If I need to shake and brake I will but I use barley seeds and no gypsum just soaked in boiling water for 90 minutes and pc cooked for like 2 hours so its kinda sticky the mycelium goes trough it though but always one side goes fast and the other stays clear longer,I hit the jars on a tire or a tape but that shit dont brake,everything else crumbles and it sticks like a huge ball of you know what I mean...It fucking stays in a ball of mycelium and just gets bruised and turns blue from all the hitting around but doesnt crumbles so that s why I am asking about shaking it or will it eventually colonize itself cause it should by all means its a fungi and it should go trough all source of food right???
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
Edited by ClintMassey (06/17/16 08:11 PM)
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dankington
The Stranger




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Re: A stupid question about colonization! [Re: ClintMassey]
#23355920 - 06/17/16 08:06 PM (7 years, 7 months ago) |
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Quote:
spacechildo said: you can use smell to confirm bacteria, but never to disprove it.
https://www.shroomery.org/forums/showflat.php/Number/23141051#23141051
Those look highly suspect. Notice the different consistencies in the mycelium? I'd say those would be best fruited in jars, with a casing... They look kinda bad.
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ClintMassey
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Re: A stupid question about colonization! [Re: dankington]
#23355951 - 06/17/16 08:21 PM (7 years, 7 months ago) |
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hmm I dont see anything wrong while looking normally thy are multi spore not from agar so yes normally there are different points where it started to grow and some growth is a little thicker some is a little fuzzieer and almost like every time I do it on pure barley seeds,I think I over cooked the seeds and maybe that is the problem I dont use brown rye or bird seeds we have barley here and pure white rye and that sucks I know,But yesterday I had even more colonised jars,I mean fully fully colonised you couldnt even see the seeds only on the bottom there were some little spots there werer visible everything was left a little longer to colonise and the mycelium just filled everything up but once I broke it it was a delight,that pure mushroomy smeel,nothing sweet or sour and that was the fastest and the crapiest looking mycelium from syzygy strain,I have 9 or 10 jars of redboy currently colonizing,another 12 of syzygy and that batch is fucked its like 5 or 6 days old,it didnt even started to show any signs of mycelium growth,the seeds are to wet and I think spores were unviable cause ŽI didnt get any mycelium growth even on popcorn grains that are over 10 days old and normal idk but that shit is messed up and this grows very nice,Can you post me a picture of your normal mycelium so I can see what do you consider normal consistency,these were started on 6,6,2016 from a syringe to that s bassicaly 12 days from inoculation and I did one thing that maybe messed the mycelium a little,usualy the temperature where I hold my jars is about 24c so I sometimes turn up a heater that heats the cupboard inside where the jars are to even 30c and then turn it off and leave it and it slowly cools down and I did that a few days for a few times a day
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
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ClintMassey
FREE RONDO

Registered: 05/31/16
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Last seen: 7 years, 7 days
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Re: A stupid question about colonization! [Re: dankington]
#23355991 - 06/17/16 08:33 PM (7 years, 7 months ago) |
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Quote:
dankington said:
Quote:
spacechildo said: you can use smell to confirm bacteria, but never to disprove it.
https://www.shroomery.org/forums/showflat.php/Number/23141051#23141051
Those look highly suspect. Notice the different consistencies in the mycelium? I'd say those would be best fruited in jars, with a casing... They look kinda bad.
Sorry I just looked at your post that you attached didnt looked straight ahead,there is certainly something fishy with that jars in your thread,that mycelium growing so far in the air and stuff,the seeds are not completely covered in mycelium which is a bad thing for me a sign of weakness or something wrong,it needs to be completely covered to be healthy mine are cmpletely covered and filling up even to the point that you dont even see the seeds so I think they are fine and by the smell of them they are pure mycelium,its is an unmistakable smell I have some contaminated jars that I used for smelling and it smeels very distinctive,one smeels super sweet,other smeels almost chemical,the moldy ones smeel...well depends on the type of mold but you know when you have a contamination I battled with a bunch of them since I started growing I think I probably had all molds that you can get and all bacterias that u can get on my agar so I smelled alot to notice the difference between the pure ones and the soury sweet pungy and the other stuff,it just smell baked and pickled somehow,I have some jars that are mix of G to G and agar together and they are somehow more suspicios to me these are the purest and best looking when you look it with a light and up close full hd hahaha just kidding,I really mean that is normaln growth I usualy get on barley seeds,on corn seeds the mycelium goes a little different and faster,barley has a different structure and is slower to colonise than the popcorn seeds but I really take care of my jars and never had a problem,I wipe them with surgical alchohol every few days,I keep them in a close environment,I even leave the tin foil after I sterilize the filter with acholhol after inoculation and everything to prevent anything from getting into my jars
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
Edited by ClintMassey (06/17/16 08:39 PM)
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ClintMassey
FREE RONDO

Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
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Re: A stupid question about colonization! [Re: ClintMassey]
#23356002 - 06/17/16 08:35 PM (7 years, 7 months ago) |
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I am pretty sure it s from the shitty camera and the flashlight,it gets all blurry and you cant see the fine details of the picture,man I need to get a better camera soon everything looks shitty and messed up when I take pictures with this piece of shit,Its an old old cell phone i think its like 2megapixels or someshit and its like disgusting how it makes everything seems so blurry
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: A stupid question about colonization! [Re: ClintMassey]
#23356012 - 06/17/16 08:38 PM (7 years, 7 months ago) |
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When mycelium looks like cottage cheese, it's not good. When you have 'colonized grains' that look wet, pressed up against the glass, it's not good. When your mycelial growth looks to have a hard edge, and a whiter shade than the rest of the myc, that's not good either.
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ClintMassey
FREE RONDO

Registered: 05/31/16
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Re: A stupid question about colonization! [Re: dankington]
#23356067 - 06/17/16 08:59 PM (7 years, 7 months ago) |
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Quote:
dankington said: When mycelium looks like cottage cheese, it's not good. When you have 'colonized grains' that look wet, pressed up against the glass, it's not good. When your mycelial growth looks to have a hard edge, and a whiter shade than the rest of the myc, that's not good either.
Thanks for the info I just examined my jars I smelled them smell is ok,nothing suspicious,the grains are a little to hydrated,I have some water drops in there I said before barley seeds I didnt quite yet experimented on the best method of obtaining a perfect balance between wet and dry seeds,but mycelium look pretty much all the same in color,except the thinner layers where it seems like it is transfering to another grain there is a visible thinner structure that is more idk that much english its more see trough until like tommorow when it covers the grain and becomes normal white like the rest,I noticed why you think it is different structured,when I inoculate I inoculate my jars with about 2cc of solution per jar i dont count but I spray in the middle and i do a all 4 side sprinkle sometimes I just sprinkle around the glass and cover it all around and it start almost always on a fwe different spots,i never get one spot that spreads but more like 6 or 7 circles that spread ad some get fuzzy type mycelium other get thicker type mycelium so they kind of stick to each other and just keep going on and on some of my jars here they havent sticked yet,I have some that are just starting but I dont wanna take every jar and take pictures now its 5 in the morning here and I really dont fell like it right now I started a diary here and in about 7 to 8 days I will post pictures of a bulk spawn I spawned so you can follow the syzygy that looked even more suspicios than this ones,that one was left to long and had clumps of mycelium and stuff but its mycelium im pretty sure,I watched you guys on the shroomery and I sometimes admire the things I see,how someone gets such beautifull growth and colonization,and especially the agar part,on that one I really admire,I never ever got that circle type growth you all get mine never grows like that,only ones I got was on agar that was a little wet so the spores kinda went with the water drops on the agar and it grows in different places and starts from there and finnally it covers the whole agar and its mycelium I even cuttedd or scraped some and smelled it and its pure mushrooms but your agar soutions or whatewer are way more better then the shit I work with here,I use mashed potato powder and agar agar and honey,and that kind of shit and I dont have the proper measurements for the base so I do it by the feel of it and things like that...Its easy for you you have pda in powder and just prepare it and malt agar extract,I need to cook my potatoes if I want that kind of agar and still dont know the right ammounts to put in the solution to pc it so I get by you know,But with the jars I almost never had and infection only infections I do get is when I open my jars to soon or do something stupid like that,idk I m tired man I just wanted to ask If I need to shake or will it eventually colonise the whole damn jar cause I know the middle is fully colonised on the most of them and I used to shake but then it needs up to 2 days to regrow the myc and stuff but I get it I need to shake and just wait it out for a 45 or 6 more days to colonise the whole damn thing
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
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ClintMassey
FREE RONDO

Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
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Re: A stupid question about colonization! [Re: ClintMassey]
#23356079 - 06/17/16 09:07 PM (7 years, 7 months ago) |
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here s ones I did with agar and G to G transfer to inoculate and clonise to see the difference it has from the thicker growth from the agar and the fuzzyer mycelium from the Grains itself,cause agar was more inoculated version and g2g was multispore so it does look kinda weird at firs,its only 4 or 4 days since I inoculated those babies and shaked them so check that out
here is the same jar 2 pictures

-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: A stupid question about colonization! [Re: ClintMassey]
#23356084 - 06/17/16 09:09 PM (7 years, 7 months ago) |
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dude these walls of text, man...
I don't need to read all that to know what I see. That popcorn may be fine, image quality makes it tough to discern.
I won't even try reading this one. Please try and be more succinct, and please format to make things easier on us. And edit posts as opposed to double posting
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ClintMassey
FREE RONDO

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Re: A stupid question about colonization! [Re: dankington]
#23356115 - 06/17/16 09:23 PM (7 years, 7 months ago) |
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Sorry for that man I m really tired like I said its 4 in the morning here and just came to check out my question about shaking and seen a bunch of pictures please so I took pictures and stuff but its shitty camera and you cant see anything and everything seems like shit when I take pictures but that s the point I was trying to make the pocorn grows one way,the barley seeds are not usual medium and they grow different than everything else and they are clearly more pure than the popcorn jars,those might have bacteria in them,they were inoculated in opened air with 2 different types of mcyelium of the same kind and they are not nearly as old as the barley jars but colonize a lot faster and have more distinct differences in growth which is visible when observing the mycelium growing from agar and the ones on the grains but whatever you say man...I grow bacteria and that was not even my question but ok nevermind,sorry for the trouble
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: A stupid question about colonization! [Re: ClintMassey]
#23356126 - 06/17/16 09:30 PM (7 years, 7 months ago) |
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You're working open-air? I'm most certain that's your issue right there. At least one of the major vectors. You really should make yourself a SAB.
But there's tons of teks on it. I even used a cardboard box with holes and a window I taped saran wrap over. Do what it takes to make things as clean as possible.
Also, try not to use popcorn, it's very inconsistent and relatively expensive. I've not had good luck with it.
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NDStepp84
Stellar nuclear waste


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Re: A stupid question about colonization! [Re: ClintMassey] 1
#23356327 - 06/17/16 11:30 PM (7 years, 7 months ago) |
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 I'd shake them, out into the trash.
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"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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VYBEv2
VYBE


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Re: A stupid question about colonization! [Re: NDStepp84]
#23356400 - 06/18/16 12:12 AM (7 years, 7 months ago) |
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Shake em out and put them in an outdoor patch if you think there could be possible contamination.
Contam aside: shake around 40-60 % unless growth slows drastically
Do not open them.... that would be contaminating them.
Hope I contributed and helped a bit.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: A stupid question about colonization! [Re: VYBEv2]
#23356769 - 06/18/16 04:26 AM (7 years, 7 months ago) |
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ur first jars of rye or wheat are super wet dude, like hot breakfast cereal, thats why they are stalling. healthy myc on wet grains can get really white like that, but urs are about to go bacterial…i don't think u could shake them loose in the jar if u tried.
ur corn is hydrated better, but because its blurry, i can't tell if its clean.
Quote:
I watched you guys on the shroomery and I sometimes admire the things I see,how someone gets such beautifull growth and colonization,and especially the agar part,on that one I really admire,I never ever got that circle type growth you all get mine never grows like that,only ones I got was on agar that was a little wet so the spores kinda went with the water drops on the agar and it grows in different places and starts from there and finnally it covers the whole agar and its mycelium I even cuttedd or scraped some and smelled it and its pure mushrooms but your agar soutions or whatewer are way more better then the shit I work with here,I use mashed potato powder and agar agar and honey,and that kind of shit and I dont have the proper measurements for the base so I do it by the feel of it and things like that...
this is kind of adorable ….did you not realize that we transfer from that first plate?? that first plate is just a teaser man….the good stuff comes down the line. next time post pictures of those plates 2-3 days after u see germination of spores, and wait until someone explains which part to transfer from and why so you can learn to identify growth yourself.
this is what clean cube spawn looks like when its almost done recovering from its final shake

trust ur filters…take off the foil. re-evaluate ur grain prep (not as wet), and make sure if ur using metal lids they have a GE hole. I like1/2" but anywhere from 1/4"-3/4" is ok.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: A stupid question about colonization! [Re: blindingleaf]
#23356779 - 06/18/16 04:38 AM (7 years, 7 months ago) |
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Gonna have to agree about the bacteria, when you get those stuck grains on the side of the jar that won't colonize completely, that is a clear bacteria contamination sign.
Don't be afraid to shake jars that have rhizo growth btw, more will form and it's one of the major advantages of using grains. Letting mycelium colonize a jar without shaking not only has the risk of stalling but it will be very slow, slow enough for the surviving bacterial endospores to recover and start to grow again before your jar is fully colonized.
Your jars might still produce mushrooms but you should manage your expectations. Bacteria will reduce yield and mold contamination is more likely with it being present. Just spawn them and hope for the best or just fruit them in the jar like darky( ) said.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
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Re: A stupid question about colonization! [Re: Supalemonhaze]
#23356835 - 06/18/16 05:29 AM (7 years, 7 months ago) |
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ClintMassey
FREE RONDO

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Re: A stupid question about colonization! [Re: bodhisatta]
#23357340 - 06/18/16 09:33 AM (7 years, 7 months ago) |
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I have shaken it this morning,we will see what will come clean what will get contaminated,I will post pics after few days when it starts to grow back...I beated the shit out of them this morning to get the colonized clumps loose,it was a hassle but i did my best know its wait and see
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: A stupid question about colonization! [Re: ClintMassey]
#23357386 - 06/18/16 09:45 AM (7 years, 7 months ago) |
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When your grains are very difficult to break up, it isn't good. 
Just another symptom.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: A stupid question about colonization! [Re: dankington]
#23357397 - 06/18/16 09:49 AM (7 years, 7 months ago) |
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if I can't shake my jars in my hands without banging them on something I don't like it.
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: A stupid question about colonization! [Re: bodhisatta]
#23357798 - 06/18/16 11:37 AM (7 years, 7 months ago) |
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Yup. If my palm isn't enough, I toss it now.
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VYBEv2
VYBE


Registered: 02/10/16
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Re: A stupid question about colonization! [Re: dankington]
#23358863 - 06/18/16 06:19 PM (7 years, 7 months ago) |
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outdoor patch outdoor patch outdoor patch......... what could it hert???
-------------------- BEEF UP YOUR CAKES [/url]  
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: A stupid question about colonization! [Re: VYBEv2]
#23358954 - 06/18/16 06:58 PM (7 years, 7 months ago) |
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Quote:
VYBEv2 said: outdoor patch outdoor patch outdoor patch......... what could it hert???
Nothing. My compost heap has several pounds of spent sub and grains...
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