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OfflineClintMassey
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Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
Re: My first bulk diary on Shroomery [Re: LocN9ne]
    #23409872 - 07/04/16 10:24 AM (7 years, 6 months ago)

Quote:

LocN9ne said:
Hey Clint... Serious question, I don't feel like reading the whole shit storm... So you PC your agar, then put peroxide on them? Then let them sit upside down to dry, THEN inoculate the plates? If I am reading that correctly, then you are accomplishing absolutely nothing except adding more water to your plates... Peroxide is H202 (water with an extra oxygen) it breaks down quickly... And if you are expecting it to accomplish anything after a couple minutes, you are going to be let down... If you are having less contams after doing this, it's most likely because your sterile procedures are improving with practice... But I promise you the peroxide isn't combatting a God Damn thing unless it is being directly applied to whatever you are trying to "clean up"



Let me correct you,I dropped something on my agar get that,I thought oh shit it will contaminaet probably I need to remove it real quick...after that the first thing I saw was antiseptic and I poured a little on my agar dish,and let it do its thing for a few seconds
after that I took the peroxide bottle to rinse out that little antiseptic liquid I spilled onto my agar plate and i rinsed it and then put the plate upside down on a dry towel inside ma SAB
Then I though why the hell I dont try to rinse the rest of my agar plates with peroxide I get alot of bacterial contaminations and I dont know why maybe this will help,so I did the same only with pure peroxide 3% and when it dried out a little I put a grain from a jar onto every plate and close the lid
Nothing grew from the grin for about 6 days and then it started to grow and there was no bacterial or any other type of contamination,what is for now the purest rate of clean agar plates I got ever.I dont know why does it always get contaminated and always bacterial contamination never mold or anything else its always the same 2 types of bacteria by the looks of it
I hope this answers your question and you get a picture of what I did and what it did with the agar I really dont know I just know it helped to prevent any type of contamination that would ussualy burst out on some of the plates
It always does so I know how much of my plates get contaminated aproximately in percentage hahaha,Idk if it helped really or not I just see the results


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I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down


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Invisiblemupetmower
Mower of Muppets
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Registered: 03/29/16
Posts: 3,036
Loc: Here and There
Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23409891 - 07/04/16 10:30 AM (7 years, 6 months ago)

dude, what dont you understand, though?? you will only cause more bacteria and contam from doing that, though.. you would have been much better off just leaving them as is, and transferring away from the bacteria. now, more than likely, you have bacteria meshed in with your myc. i can almost guarantee that you do. it might LOOK clean to you, but it probably isnt.

the first thing you should be doing, is assessing your sterile technique. make some blank plates, and do some test transfers, and see if anything grows. if it does, then you need to fix your sterile technique.


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-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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OfflineClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
Re: My first bulk diary on Shroomery [Re: dankington]
    #23409922 - 07/04/16 10:41 AM (7 years, 6 months ago)

Quote:

dankington said:
that's just it, clint. my methods are not my own. I've just read, and read and follow what others before me have tried. it's as easy as spores>agar>agar>grains>CVG.



Look here,I ve noticed somethings here,almost everybody uses a giant SAB or a flow hood when they do their stuff,I know it is spores,to agar,than clean part of mycelium,than the best part of mycelium,than you inoculate that with that isolated mycelium and get very unique and rapid growth and stabile appearance and looks,more unique fruits and better yields...or cloning the one fruit you like by apearrance and get just that type of growth I get that isolating part
but first thing,I cannot get any petri dishes in my country,it doesnt sells everywhere,you cannot find a store that sells that laboratory equipment,I looked on ebay and the shipment is more than the dishes itself,I use medical cups for piss,they are sterile and small so I figured ok that will do,problem is in this
they are so tall and have a large cap that is very difficult to move and drop what you need to drop cause the cups are pretty deep comared to the width so I have some problems with that,I get much contaminations even when I work in sab,I clean my whole room before I work
I turn on the air purifier that has a hepa filter and other type of filters to clean out shit that s floating in the air as much as possible!
I turn that shit off and then slowly I start prepairing when the air becomes still in the room,No windows open,doors closed,I put my facemask,I sterilize my hands to the elbows,I put on glowes and sanitize them afterwards,I clean my sab from within with the same medical shit,Then i clean the doors and ouside of the box,I clean the table Im working on,I put the sab on the table,I put everything in my sab and close the hand holes
Then I start working,at first I tryied everything as fast as I can now I got the point that the slower moves you make the air stays more still so I do it slowly,I try not to fuck up and do it with first try,i dont open lids then work,and that kind of stuff I prepare everything and just open lid put grain close lid,But it still gets contaminated,I improved a little from the point I started but its still not good enough its simple as that!
Sorry for the long post but Im trying to explain that I understand the theory and I read alot but I still need to practice alot and do alot of transfers and try out alot of different stuff until I perfect that stuff
I know that with time I will perfect everything cause I got addicted to this stuff and learning about this is very amusing and I like it and understand it pretty easily,Its just a matter of time and til then I will post shitty grows :grin:


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I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down


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Invisibledankington
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Registered: 03/14/15
Posts: 4,577
Loc: 8te
Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23409927 - 07/04/16 10:43 AM (7 years, 6 months ago)



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Invisibledankington
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Registered: 03/14/15
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Re: My first bulk diary on Shroomery [Re: dankington]
    #23409933 - 07/04/16 10:45 AM (7 years, 6 months ago)

there're always ways, my man. I use pre-pour still (pastyplates), and I manage.


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OfflineClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
Re: My first bulk diary on Shroomery [Re: mupetmower]
    #23409945 - 07/04/16 10:49 AM (7 years, 6 months ago)

Quote:

mupetmower said:
dude, what dont you understand, though?? you will only cause more bacteria and contam from doing that, though.. you would have been much better off just leaving them as is, and transferring away from the bacteria. now, more than likely, you have bacteria meshed in with your myc. i can almost guarantee that you do. it might LOOK clean to you, but it probably isnt.

the first thing you should be doing, is assessing your sterile technique. make some blank plates, and do some test transfers, and see if anything grows. if it does, then you need to fix your sterile technique.



I will work on that,I am trying to work on that its just I dont have petri dishes,and any more plates,I gotta go buy that piss containers until I have a chance to order a bunch of petri dishes,I dont know and had never seen a bacterial growth that you cannot see on agar though...I usualy get white milky growth or slightly yellowish spots and that kind of stuff,I we also noticed that you can spot when mycelium grows on the edge it spreads little visible threads that doesnt like to get into the area that is contaminated with bacteria or mold,I think I can spot bacteria on agar plates,I ve seen enough contaminations but never saw no ghost type of bacteria that you cannot spot on agar,it would spread and make some type of growth for sure so I think its not,I mean I dont know cause i cant see anything except mycelium,If its safe to open a fully colonized jar of mycelium without fear of contamination I will open and take a hq picture of my culture with some underneath light so you can observe the threads and the growth patterns


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I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down


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Invisibledankington
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Registered: 03/14/15
Posts: 4,577
Loc: 8te
Re: My first bulk diary on Shroomery [Re: dankington]
    #23409952 - 07/04/16 10:51 AM (7 years, 6 months ago)

Quote:

dankington said:
how about this:?
https://www.shroomery.org/forums/showflat.php/Number/23218339#23218339




those are good sized containers you can most likely find if you're in the EU...

you can also add food coloring to the agar to help make bacteria more visible.


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OfflineClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
Re: My first bulk diary on Shroomery [Re: dankington]
    #23409958 - 07/04/16 10:54 AM (7 years, 6 months ago)

Quote:

dankington said:
there're always ways, my man. I use pre-pour still (pastyplates), and I manage.



Yea I know about pastywhite s no pour tek I looked it up,amazing stuff only I cant seem to find that type and brand of plastic containters that are small,I tried that pp5 and they melted in my pressure cooker so I guess it was some chinese shit of plastic or something  :rofl:


--------------------
I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down


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OfflineClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23409962 - 07/04/16 10:55 AM (7 years, 6 months ago)

and it is balcan dude,it is eu only in theory,its a little different in practice hahahahaha


--------------------
I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down


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OfflineClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
Re: My first bulk diary on Shroomery [Re: dankington]
    #23409968 - 07/04/16 10:58 AM (7 years, 6 months ago)

Quote:

dankington said:
Quote:

dankington said:
how about this:?
https://www.shroomery.org/forums/showflat.php/Number/23218339#23218339




those are good sized containers you can most likely find if you're in the EU...

you can also add food coloring to the agar to help make bacteria more visible.



I got those only a little bigger size,there werent any smaller,mine are like 15cm or something


--------------------
I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down


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OfflineClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23409979 - 07/04/16 11:00 AM (7 years, 6 months ago)

I got an idea,How about this
What if I make a youtube channel and post videos of my equpiment and things I do so I can post every procedure and you guys can tell me where I do things wrong,and If I am missing something you can correct me I think it would be the best and the easiest way to learn and correct my procedures!


--------------------
I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down


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Invisiblethe_r3dz
Trich Propagator
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Registered: 03/26/15
Posts: 5,709
Re: My first bulk diary on Shroomery [Re: ClintMassey] * 1
    #23410009 - 07/04/16 11:10 AM (7 years, 6 months ago)

Because you're a lot safer here on the shroomery, why not just take pics of your stuff if you want us to see it?


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InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: My first bulk diary on Shroomery [Re: ClintMassey] * 1
    #23410016 - 07/04/16 11:14 AM (7 years, 6 months ago)

Hey dude, you might wanna take a look at www.ampulla.eu for pp5's. They have good quart jars as well, and quite cheap. You would do well to squeeze in a few pp5s if you were ever to buy jars from them. Shipping isn't cheap but it was well worth it for me. I have about 80 quart jars from them and like 350 agar pp5s cause I just fell in love with them. I have a tek in my sig on how to go about using them.

As for pp5s melting, they were probably a mix of plastic. Proper pp5 shouldn't do that, regardless of where it's made of. Most plastics are made in china anyway. They should really be recycle code 7 if they were a mix but IDK if it's a marketing ploy or something.

Anyway, you should still be able to find something in supermarkets, we get loads of them here and I live in a country were supplies really hard to come by. Everything is expensive locally though so for jars and pp5s, I just stick to ampulla.

If you ever decide to buy from them and the shipping goes up to much, pm me because I found a neat little loophole in their site where you can buy more for less shipping costs.


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OfflineClintMassey
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Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
Re: My first bulk diary on Shroomery [Re: Supalemonhaze]
    #23410080 - 07/04/16 11:35 AM (7 years, 6 months ago)

Quote:

Supalemonhaze said:
Hey dude, you might wanna take a look at www.ampulla.eu for pp5's. They have good quart jars as well, and quite cheap. You would do well to squeeze in a few pp5s if you were ever to buy jars from them. Shipping isn't cheap but it was well worth it for me. I have about 80 quart jars from them and like 350 agar pp5s cause I just fell in love with them. I have a tek in my sig on how to go about using them.

As for pp5s melting, they were probably a mix of plastic. Proper pp5 shouldn't do that, regardless of where it's made of. Most plastics are made in china anyway. They should really be recycle code 7 if they were a mix but IDK if it's a marketing ploy or something.

Anyway, you should still be able to find something in supermarkets, we get loads of them here and I live in a country were supplies really hard to come by. Everything is expensive locally though so for jars and pp5s, I just stick to ampulla.

If you ever decide to buy from them and the shipping goes up to much, pm me because I found a neat little loophole in their site where you can buy more for less shipping costs.




Thank you, I just browsed the site and shipping costs to my country are 3100 €.


--------------------
I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down


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InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23410091 - 07/04/16 11:38 AM (7 years, 6 months ago)

Say what? :lolwut:


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InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: My first bulk diary on Shroomery [Re: Supalemonhaze]
    #23410107 - 07/04/16 11:45 AM (7 years, 6 months ago)

Did you actually try to put something in the shopping cart and checkout? It will tell you the final shipping price before you have to pay.

They charge me like 30euro for 30kilos, I can buy somewhere around 50-60 jars for 30 euro shipping so it's not too bad considering that a quart jar locally costs 3.50euro each. I just saw that they removed the quart jar from inventory though, I hope they get it back.


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OfflineClintMassey
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Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
Re: My first bulk diary on Shroomery [Re: Supalemonhaze]
    #23410123 - 07/04/16 11:53 AM (7 years, 6 months ago)

Quote:

Supalemonhaze said:
Say what? :lolwut:




Yes, I did.

Standard Delivery - Despatched within 7 days € 3125.00

:rofl:


--------------------
I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down


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InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: My first bulk diary on Shroomery [Re: ClintMassey]
    #23410130 - 07/04/16 11:55 AM (7 years, 6 months ago)

HOLY SHIT. Send them an email and very kindly ask which type of drugs they are taking and where we can get some. I wanna be on whatever it is they are on.


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InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: My first bulk diary on Shroomery [Re: Supalemonhaze]
    #23410143 - 07/04/16 12:00 PM (7 years, 6 months ago)

You should still be able to find some good PP5s locally though so don't fret. You got unlucky with the ones that melted, it is not very common for that to happen. Big ass supermarkets is where you wanna check. Normally here, the ones that are pp5 will be advertised as "microwaveable". Just don't buy non pp5 if it says it's microwavable, it will probably melt anyway. 100% pp5 should not melt,regardless of how thin they are.

The ones I buy from ampulla have really thin lids, they warp a little if you don't space the lids out with a papertowel but if you do that, they handle it like champs.


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OfflineClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
Re: My first bulk diary on Shroomery [Re: Supalemonhaze]
    #23410185 - 07/04/16 12:16 PM (7 years, 6 months ago)

Quote:

Supalemonhaze said:
You should still be able to find some good PP5s locally though so don't fret. You got unlucky with the ones that melted, it is not very common for that to happen. Big ass supermarkets is where you wanna check. Normally here, the ones that are pp5 will be advertised as "microwaveable". Just don't buy non pp5 if it says it's microwavable, it will probably melt anyway. 100% pp5 should not melt,regardless of how thin they are.

The ones I buy from ampulla have really thin lids, they warp a little if you don't space the lids out with a papertowel but if you do that, they handle it like champs.




I know about PP5 plastics, I checked it all before.. Can you please stick around, I'll post a pic of my pins soon and I need some info.


--------------------
I just move myself around, away from the crowd
Cause the crowd get shot down, and I don't get shot down


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