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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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I save this thread to read on the shitter.
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ClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
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I am not dipping my substrate in peroxide,you got something wrong,I just clean my agar surface with 3% peroxide and leave it of a while to dry out a little,it leaves a very clean smooth surface...I put regular grains not dipped in peroxide,I leave them until the new growth appears on agar and just watch them every day to see is there any contamination and for now it showed great,only mycelium spreaded and no bacterias or any type of that shit...and yea man I am illiterate cause I dont usually speak english,sorry you didnt understand my question fully so I dont find your answer appropriate to the question but thanks alot,I am glas you found something funny in everything
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
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ClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
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Re: My first bulk diary on Shroomery [Re: Inocuole]
#23409245 - 07/04/16 03:44 AM (7 years, 6 months ago) |
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Quote:
Inocuole said: I save this thread to read on the shitter.
You know what dude, there is a saying in my country and it goes like this...svaka reklama je dobra reklama on english it would go something like this Any advertising is good advertising enjoy on the shitter,I guess you spend most of your time there...just kidding a little...I would say something to you now but its not worth it,live in your world and enjoy it...future is a motherfucker and karma is a bitch enjoy
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
Edited by ClintMassey (07/04/16 04:36 AM)
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drolman
Dimension Jumper

Registered: 03/01/14
Posts: 64
Loc: Somewhere between the 2nd...
Last seen: 3 months, 6 days
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
#23409252 - 07/04/16 03:49 AM (7 years, 6 months ago) |
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Good luck, I hope it is not a contam and it fruits for you. I've been following.
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ClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
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Re: My first bulk diary on Shroomery [Re: drolman]
#23409317 - 07/04/16 04:59 AM (7 years, 6 months ago) |
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Quote:
drolman said: Good luck, I hope it is not a contam and it fruits for you. I've been following.
Ty!
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
#23409360 - 07/04/16 05:36 AM (7 years, 6 months ago) |
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Quote:
ClintMassey said: And here is the proof that it is wise to rinse your agar surface with H202 and let it uspide dry out a bit then do your transfers without fearing that you will transfer bacteria from a contaminated source or fear contaminations

usually "proof" in this hobby means mushrooms, not blurry unzoomable pics uploaded to another site...
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ClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
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Re: My first bulk diary on Shroomery [Re: spacechildo]
#23409524 - 07/04/16 07:37 AM (7 years, 6 months ago) |
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Spacechildo I am really sorry but this site will not upload my pictures I tried before posting on image upload and I dont feel like waiting for ressurecton of christ for a few pictures to upload so I can get codes
you can zoom and observe,but im already sick of your smart ass speaches if you dont like what you see please leave you are not helping in any way only spamming my thread,plz dont troll if you dont have nothing smart to say,you can clearly see its very much purer and cleaner than that opened already contaminated lame ass 2 day old normal agar g2g transfered jar,sorry jars are a little blurry,I will gladly open my culture and give you a high resolution image of the mycelium culture once I perform isolation to another agar jar until then can you please stop with your being a dick routine...
I get that you are just mad cause you are wrong about the peroxide and trying to turn out as the smart one by sneak dissing when someone is proving you wrong in front people
I promise if anyone else starts shitting about the f...ing peroxide I will make a detailed video with 10 agar + peroxide and 10 pure agar dishes and perform g2g transfer on all of them and literally quote you and others and make a frickin tek for making contamination free agar plates and prove you are wrong...please just stop I m not here to discuss or to argue with anybody about anything I just dont like narrow sighted persons who dont have real knowledge but nearly just spread informations they collected from other people and really dont know shit about what are they talking about,because they cannot even explain why are they saying what are they saying and prove that they are right
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
Edited by ClintMassey (07/04/16 07:47 AM)
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
#23409530 - 07/04/16 07:39 AM (7 years, 6 months ago) |
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Quote:
ClintMassey said: but im already sick of your smart ass speaches if you dont like what you see please leave you are not helping in any way only spamming my thread,plz dont troll if you dont have nothing smart to say,
please stop with your being a dick routine,you are just mad cause you are wrong and someone is proving that
no, its the other way around, you're being a dick spreading bad info like gospel, not knowing what you're doing yet but still thinking hey I should share these fucked up thoughts on cultivation.
I'm helping big time, I'm helping others not fall for your BS.
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ClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
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Re: My first bulk diary on Shroomery [Re: spacechildo]
#23409549 - 07/04/16 07:53 AM (7 years, 6 months ago) |
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Then my friend I dare you to prove that more diluted or even 3% H202 solution mixed with water hurts mycelium in any way,scinetifically it cannot be proven that is to small concetration to do anything to mycelium
http://www.fanaticus.com/antibiot.htm
here is some reading on antibiotics and peroxide if you havent read,I would like to see your proof that h202 will hurt mycelium,make a test yourself and prove to everybody you are right
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mrmazdarx9
Pffffttt


Registered: 05/15/16
Posts: 9,796
Loc: behind you
Last seen: 1 year, 4 months
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
#23409554 - 07/04/16 07:55 AM (7 years, 6 months ago) |
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You posted pics earlier in this thread so full of shit it went wrong and you don't wanna admit you were wrong Probably waiting for a batch you did right to fruit then post pics on that Either post pics or it didn't happen
-------------------- COCA GROWERS come here and share your knowledge COCA GROWERS UNITE
Click here for UK trades
need some supplies in the UK check Here or PM me UK OTD uk members chat UK supplies and trade OTD place to chat shit Right Here If you use "SWIM" you should DROWN
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ClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
#23409558 - 07/04/16 07:56 AM (7 years, 6 months ago) |
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Hydrogen Peroxide is a powerful antiseptic. The solution of Hydrogen Peroxide bought in a drug store is 3% Hydrogen Peroxide and 97% water. Even at this low concentration, and with further dilution's, the germ killing is potent. But that germ killing power only works for micro fungi spores and bacteria endospores. A micro-organism that has germinated into its secondary form (mycelium), is safe from the antiseptic power of diluted Hydrogen Peroxide. But the ungerminated spores, bacteria endospores, and microbes are all susceptible. If there is bacteria that is growing (germinated), it will not succumb to the peroxidated water (just as the fungi mycelium is not succumbing). Also, any mold that is growing will survive. A clean fragment of shroom flesh or mycelium from a mold contaminated culture has germs all over it, but only in the spore or endospore form. They won't germinate in the peroxidated medium and water or on the recovering mycelia.
The tek is like a tightrope act. The mycelium that is cultured in the peroxide enriched medium can survive and grow, but it is not clean. Any spores or bacteria that are "piggy-backing" on the mycelium and not in contact with the peroxidated medium can come to life if given the opportunity.
After the culture of mycelium grows, it can be rehydrated with more peroxidated water. The spores and bacteria endospores that are "piggy-backing" on the mycelium will die. The mycelium in its fully secondary form, will survive the new peroxidated solution, "cleaned".
Professor Fanaticus
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
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ClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
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Re: My first bulk diary on Shroomery [Re: mrmazdarx9]
#23409560 - 07/04/16 07:57 AM (7 years, 6 months ago) |
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Quote:
mrmazdarx9 said: You posted pics earlier in this thread so full of shit it went wrong and you don't wanna admit you were wrong Probably waiting for a batch you did right to fruit then post pics on that Either post pics or it didn't happen
Post pics of what exactly???
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: My first bulk diary on Shroomery [Re: ClintMassey] 2
#23409569 - 07/04/16 08:04 AM (7 years, 6 months ago) |
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Quote:
ClintMassey said: Then my friend I dare you to prove that more diluted or even 3% H202 solution mixed with water hurts mycelium in any way,scinetifically it cannot be proven that is to small concetration to do anything to mycelium
http://www.fanaticus.com/antibiot.htm
here is some reading on antibiotics and peroxide if you havent read,I would like to see your proof that h202 will hurt mycelium,make a test yourself and prove to everybody you are right
h2o2 is used to treat cob web for newbs who get cobweb and bother trying to fix it. nothing else. been proven over and over again. Not sure what you think that link says  You're the one making wild claims, so You're the one who has to prove it.
example; God exists my proof is; You try to disprove him then...
just doesnt work that way...
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ClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
#23409576 - 07/04/16 08:09 AM (7 years, 6 months ago) |
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god damn what an idiot you are mrmazdarx9 do you think I have time to post pictures of 3 identical 2 liter trays of my only left spawn of syzygy that I had and even said they were my last colonies and i wont let them get ruined,and somene started with that looks contaminated talk cause the mycelium looked different than on his perlite vermiculite casing or whatever and then I magically used my powers of materialising and summoned another 3 identical cakes of syzygy with pins and came here claiming that I did something that U personaly consider bullshit cause look now there are pins growing out on the cakes and everyone thought and said it would be fucked up,stop being a retard please,I dont got time for your trolling and shit cause you are stubborn and never tried anything else that makes common sense in a situation and you only know how to follow teks and dont know how to think for yourself?fine than it didnt happen take a walk dude and stop coming to this thread
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
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ClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
#23409600 - 07/04/16 08:23 AM (7 years, 6 months ago) |
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Ok its not like you said,it is used in medicine and agriculture and I had this kind of fights with idiots on cannabis growing forums for another purpose it is used by some of smart people in fungiculture even but ok,I accept your challenge, I will make a video in which i will cook the agar ammount for 20 jars and pour it into same jars in sab all recording non stop I will let the agar cool down I will treat 10 agar plates and mark them so it cannot be erased or swaped,and I will leave 10 other jars untreated After I treat the 10 jars with H202 and drain them out I will inoculate all 20 from the same jar of grains with a single grain of colonized spawn After that I will take pictures every single day of the both batches of agar for 2 weeks so you can see that it takes 6 days for mycelium to starts growing inside the H202 treated jars,and we shall see how many of them will be clean once the test ends in about 14 days from the start
If my theory proves its point and creates better results and be 100% clear you will make a new thread and appologize publicly and admit you are an idiot If your theory proves its point I will leave this forum forever and never return? Are you ready to take on the challenge?
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
#23409636 - 07/04/16 08:45 AM (7 years, 6 months ago) |
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Quote:
ClintMassey said: Ok its not like you said,it is used in medicine and agriculture and I had this kind of fights with idiots on cannabis growing forums for another purpose it is used by some of smart people in fungiculture even but ok
this is mush cult. h2o2 has no place in sterile tech. Please show me which "smart people" use peroxide for mush cult! should be good  you're not the first one to find bad info or read what you want into the stuff you find. if you think h2o2 kills endospores go ahead and "sterilize" your grains with h2o2. I'll be here 
sanitizing stuff is not sterile tech. heat kills, cleaners sanitizes. removing 99,999% of 100 bn mold spores is far from good enough.
stop using meth and start using the enter button, try to think out what you wanna say before typing and use periods, dont double post and try to focus.
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ClintMassey
FREE RONDO


Registered: 05/31/16
Posts: 178
Last seen: 7 years, 7 days
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Re: My first bulk diary on Shroomery [Re: spacechildo]
#23409663 - 07/04/16 08:55 AM (7 years, 6 months ago) |
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Quote:
spacechildo said:
Quote:
ClintMassey said: Ok its not like you said,it is used in medicine and agriculture and I had this kind of fights with idiots on cannabis growing forums for another purpose it is used by some of smart people in fungiculture even but ok
this is mush cult. h2o2 has no place in sterile tech. Please show me which "smart people" use peroxide for mush cult! should be good  you're not the first one to find bad info or read what you want into the stuff you find. if you think h2o2 kills endospores go ahead and "sterilize" your grains with h2o2. I'll be here 
sanitizing stuff is not sterile tech. heat kills, cleaners sanitizes. removing 99,999% of 100 bn mold spores is far from good enough.
stop using meth and start using the enter button, try to think out what you wanna say before typing and use periods, dont double post and try to focus.
What are U my fucking momma,I dont do meth,I dont do drugs actually,I dont even drink. Stop acting a fool and just take the challenge publicly,and do I have to spell it I am not a fucking U.S.A. resident and I am not british...English is not my native language If you want to start this beef than just say the words and I will make a youtube channel and cook the fucking agar tonight,I accidentally have some malabar and redboy grains colonized so I will gladly shoot that shitty half hour video of the procedure and then we will see how much effective is my peroxide against your words
-------------------- I just move myself around, away from the crowd Cause the crowd get shot down, and I don't get shot down
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: My first bulk diary on Shroomery [Re: ClintMassey]
#23409669 - 07/04/16 08:57 AM (7 years, 6 months ago) |
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peroxide. it works by causing cell walls to collapse and the weight of the cell can't hold itself any longer. Mycelium can withstand it at 3% concentrations, but it will still weaken the cell walls significantly. we all told you you would most likely get some fruits. But there're reasons we told you to put the H2O2 away.
I realize you think we're all potheads and shroomheads and probably always fucked up. But some of us are fairly intelligent--we learn from the legends that came before us.
you don't think any of us have heard of Fanaticus? Think we just tell people to do PF tek without knowing who PF was?
You're right, PF did a lot of work with antibiotics and H2O2. Did you know his specimens, all his work, all his genetics are deteriorating due to a degenerative virus that started attacking his weakened cultures?
Here's a quote from Workman, one of the truly great mycologists here: https://www.shroomery.org/forums/showflat.php/Number/7504640#7504640
Quote:
Workman said: The bent stems could be genetic or it might be a viral infection. I have long suspected that the PF strains harbor a virus that causes deformity. I am currently trying to inactive any potential virus though a heat therapy protocol to see if that improves the form. If the problem is only genetic it should be improved by crossing with healthier strains. Sorry, I didn't save any for a cross section but they do blue intensely at the slightest touch.
here's an interview too: https://www.shroomery.org/forums/showflat.php/Number/6720321#6720321
If you'll notice, Workman had to do a lot of work to try and save these cultures. Because of repeated uses of antibiotics, peroxide washes, and perhaps as theorized by some, the over use of blacklights for growing.
in the future, save the H2O2 for the only thing we'll ever use it for: Dactylium infections.
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: My first bulk diary on Shroomery [Re: dankington]
#23409680 - 07/04/16 09:01 AM (7 years, 6 months ago) |
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also, peroxide turns into something crazy when exposed to air for a period of time! water and oxygen!
Edited by dankington (07/04/16 09:11 AM)
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Re: My first bulk diary on Shroomery [Re: dankington]
#23409684 - 07/04/16 09:04 AM (7 years, 6 months ago) |
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Hey Clint... Serious question, I don't feel like reading the whole shit storm... So you PC your agar, then put peroxide on them? Then let them sit upside down to dry, THEN inoculate the plates? If I am reading that correctly, then you are accomplishing absolutely nothing except adding more water to your plates... Peroxide is H202 (water with an extra oxygen) it breaks down quickly... And if you are expecting it to accomplish anything after a couple minutes, you are going to be let down... If you are having less contams after doing this, it's most likely because your sterile procedures are improving with practice... But I promise you the peroxide isn't combatting a God Damn thing unless it is being directly applied to whatever you are trying to "clean up"
--------------------
Q&A US vs. THEM The more I learn, the less I know.
Edited by LocN9ne (07/04/16 09:14 AM)
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