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Offlineshanesean023
NewGuy
Male
Registered: 04/29/16
Posts: 117
Last seen: 6 years, 10 months
Re: What NOT to do [Re: Kenetic]
    #23368633 - 06/21/16 11:08 PM (7 years, 7 months ago)

Quote:

kenetic said:
Hey dude you don't have to act like a pretentious asshole.  You cleary don't know what you are talking about.
For starters, how do you consider a fruiting chamber sterile?  Your use of this word in this context makes it evident that you don't understand what this word actually means. 
There are much better ways to save a species than a dirty cotton swab.
I'm not lazy, I'm simply smarter than you.  I know not to waste my time with an uncontrolled variable when I can use proven methods.





Did I sat it was? I think it's pretty evident the sterile environment is the swab and everything you do with it. What are your better and proven methods since apparently no one has a proved way of using a sterile spore swab????


--------------------
Looking for a good Mexicana species, I have alot to trade.

This account is strictly for role-playing purposes! Nothing that is said, posted, mentioned, or messaged is fact based and has never actually happened. Thanks for playing along.


Edited by shanesean023 (06/21/16 11:23 PM)


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OfflineNDStepp84
Stellar nuclear waste
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Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
Re: What NOT to do [Re: shanesean023]
    #23368653 - 06/21/16 11:17 PM (7 years, 7 months ago)



--------------------

"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do.
-Robert A. Heinlein 

:takingnotes: Links and teks:takingnotes:
ND's grow log and discussion
Plant thread


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OfflineGr0wer
always improving
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Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX Flag
Last seen: 5 years, 10 months
Trusted Cultivator
Re: What NOT to do [Re: NDStepp84]
    #23368726 - 06/21/16 11:41 PM (7 years, 7 months ago)

Only issue i see is some black teeth....


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OfflineMMG
Artesano
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Registered: 07/29/15
Posts: 547
Last seen: 2 years, 3 months
Re: What NOT to do [Re: Gr0wer]
    #23368770 - 06/21/16 11:54 PM (7 years, 7 months ago)

and fingers.
dig the idea of making chocolate with mushrooms, I'm gonna try it soon.


--------------------
:heart:
  :psychsplit:
    :heart:


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Invisiblemupetmower
Mower of Muppets
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Registered: 03/29/16
Posts: 3,036
Loc: Here and There
Re: What NOT to do [Re: shanesean023]
    #23369302 - 06/22/16 08:18 AM (7 years, 7 months ago)

Quote:

shanesean023 said:
Quote:

kenetic said:
Hey dude you don't have to act like a pretentious asshole.  You cleary don't know what you are talking about.
For starters, how do you consider a fruiting chamber sterile?  Your use of this word in this context makes it evident that you don't understand what this word actually means. 
There are much better ways to save a species than a dirty cotton swab.
I'm not lazy, I'm simply smarter than you.  I know not to waste my time with an uncontrolled variable when I can use proven methods.





Did I sat it was? I think it's pretty evident the sterile environment is the swab and everything you do with it. What are your better and proven methods since apparently no one has a proved way of using a sterile spore swab????





dude... :facepalm:

you obviously really dont know the meaning of the word sterile.. if you take a STERILE swab, and swab DIRTY spores off a mushroom cap in a NON-STERILE FC, then it isnt sterile anymore, is it?

and on another note, just because you posted you tube videos of congressmen saying something, doesnt mean it is absolute truth. You need actual factual evidence, and some congressman saying shit isnt that.

I cant tell if youre just a troll, or really this ignorant? But I can tell that you deserve the rating you have,


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.


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OfflineSloppyJoseph
Non-sporalator
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Registered: 12/08/15
Posts: 8,511
Loc: Flag
Last seen: 3 years, 7 months
Re: What NOT to do [Re: Gr0wer]
    #23369323 - 06/22/16 08:28 AM (7 years, 7 months ago)

Quote:

Gr0wer said:
Only issue i see is some black teeth....



Yeah, I guess it's not a big deal for some but I'm a bit of a perfectionist. I like my hard work to not look like the black death. And it was gonna be a pretty nice looking dub too, missed the kodak moment. And what else do we have on here except our pictures? :p


--------------------
AMU Q&A


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Offlineshanesean023
NewGuy
Male
Registered: 04/29/16
Posts: 117
Last seen: 6 years, 10 months
Re: What NOT to do [Re: mupetmower]
    #23369753 - 06/22/16 11:53 AM (7 years, 7 months ago)

Quote:

mupetmower said:
Quote:

shanesean023 said:
Quote:

kenetic said:
Hey dude you don't have to act like a pretentious asshole.  You cleary don't know what you are talking about.
For starters, how do you consider a fruiting chamber sterile?  Your use of this word in this context makes it evident that you don't understand what this word actually means. 
There are much better ways to save a species than a dirty cotton swab.
I'm not lazy, I'm simply smarter than you.  I know not to waste my time with an uncontrolled variable when I can use proven methods.





Did I sat it was? I think it's pretty evident the sterile environment is the swab and everything you do with it. What are your better and proven methods since apparently no one has a proved way of using a sterile spore swab????





dude... :facepalm:

you obviously really dont know the meaning of the word sterile.. if you take a STERILE swab, and swab DIRTY spores off a mushroom cap in a NON-STERILE FC, then it isnt sterile anymore, is it?

and on another note, just because you posted you tube videos of congressmen saying something, doesnt mean it is absolute truth. You need actual factual evidence, and some congressman saying shit isnt that.

I cant tell if youre just a troll, or really this ignorant? But I can tell that you deserve the rating you have,






You obviously don't know shit if I have to spoon feed you every lil damn thing. OK take a box a q tips place in GB spray with oust wait 5 minutes and what do you have moron


--------------------
Looking for a good Mexicana species, I have alot to trade.

This account is strictly for role-playing purposes! Nothing that is said, posted, mentioned, or messaged is fact based and has never actually happened. Thanks for playing along.


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Offlinespacechildo
proletarians rise up
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Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: What NOT to do [Re: shanesean023]
    #23369772 - 06/22/16 12:00 PM (7 years, 7 months ago)

swabs without spores on them arent worth much to me, spores aren't clean are what people are saying so once you swab a sterile swab on a cap or print its no longer sterile :super:

agar is great for making sure you have a clean inoculant, not daring to store the culture in fear of a power-out doesnt take that away!

oh and btw, lysoling or ousting a q-tip doesnt make it sterile, PC'ing it does.


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Offlineshanesean023
NewGuy
Male
Registered: 04/29/16
Posts: 117
Last seen: 6 years, 10 months
Re: What NOT to do [Re: spacechildo]
    #23369790 - 06/22/16 12:05 PM (7 years, 7 months ago)

Quote:

spacechildo said:
swabs without spores on them arent worth much to me, spores aren't clean are what people are saying so once you swab a sterile swab on a cap or print its no longer sterile :super:

agar is great for making sure you have a clean inoculant, not daring to store the culture in fear of a power-out doesnt take that away!

oh and btw, lysoling or ousting a q-tip doesnt make it sterile, PC'ing it does.





Exactly that's why I said to transfer to the LC to agar. I said do x transfers on agar to clean up your mycelium. An keep the LC as a almost limitless supply of said strain and a safe backup that can be, will be, and is cleaned in the end


--------------------
Looking for a good Mexicana species, I have alot to trade.

This account is strictly for role-playing purposes! Nothing that is said, posted, mentioned, or messaged is fact based and has never actually happened. Thanks for playing along.


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Offlineshanesean023
NewGuy
Male
Registered: 04/29/16
Posts: 117
Last seen: 6 years, 10 months
Re: What NOT to do [Re: shanesean023]
    #23369794 - 06/22/16 12:08 PM (7 years, 7 months ago)

Everytime I PC q tips they swell big af or or become flimsy af. I'll take my chances with .001% germs and bacteria.


--------------------
Looking for a good Mexicana species, I have alot to trade.

This account is strictly for role-playing purposes! Nothing that is said, posted, mentioned, or messaged is fact based and has never actually happened. Thanks for playing along.


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Offlinespacechildo
proletarians rise up
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Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: What NOT to do [Re: shanesean023]
    #23369797 - 06/22/16 12:09 PM (7 years, 7 months ago)

from LC to agar is the wrong way, clean a culture up on agar and then throw wedges in grain jars or LC, and a small transfer to a slant if its a clone or culture you wanna keep.

if you dont sterilize your Q-tip you shouldn't use it, flame a loop instead.


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Offlineshanesean023
NewGuy
Male
Registered: 04/29/16
Posts: 117
Last seen: 6 years, 10 months
Re: What NOT to do [Re: spacechildo]
    #23369822 - 06/22/16 12:17 PM (7 years, 7 months ago)

That's a great way. Wouldn't you still want to clean that LC on agar everytime before you go to grain or GLC? So you'd be starting right back at LC to agar to GLC.But yes agar to LC would definitely be best to start


--------------------
Looking for a good Mexicana species, I have alot to trade.

This account is strictly for role-playing purposes! Nothing that is said, posted, mentioned, or messaged is fact based and has never actually happened. Thanks for playing along.


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InvisibleInocuole
Scalpel of Evil's Bane
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Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: What NOT to do [Re: shanesean023]
    #23369876 - 06/22/16 12:44 PM (7 years, 7 months ago)

Actual Q tips can be sterilized pretty easily but I use the pre-sterile wooden ones in individual sleeves.  I also only take spores from sterile invitro fruits.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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InvisibleLocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ
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Registered: 04/17/15
Posts: 7,076
Loc: to the brain Flag
Re: What NOT to do [Re: shanesean023]
    #23369941 - 06/22/16 01:11 PM (7 years, 7 months ago)

Quote:

shanesean023 said:
Everytime I PC q tips they swell big af or or become flimsy af. I'll take my chances with .001% germs and bacteria.



That's just the amount needed to Fuck ya... Weird how that works out... You need to individually wrap the qtips in foil, then put them in a jar and PC them... They should still be dry after the PC cycle man...


--------------------


Q&A
US vs. THEM

The more I learn, the less I know.


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Offlinespacechildo
proletarians rise up
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Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Re: What NOT to do [Re: shanesean023]
    #23369969 - 06/22/16 01:27 PM (7 years, 7 months ago)

Quote:

shanesean023 said:
That's a great way. Wouldn't you still want to clean that LC on agar everytime before you go to grain or GLC? So you'd be starting right back at LC to agar to GLC.But yes agar to LC would definitely be best to start




no, not at all. a dirty LC is trash, its just too much work cleaning it up on agar. and LCs are used to noc up grains, there's not much point in going to LC if you're just gonna use it to start an agar wedge.
You're just going in a loop that makes no sense, where does the GLC come into play??


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InvisibleInocuole
Scalpel of Evil's Bane
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Re: What NOT to do [Re: spacechildo]
    #23369985 - 06/22/16 01:32 PM (7 years, 7 months ago)

LC to agar to GLC. :rofl:  What a waste of time..


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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Offlinegravybrain
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Registered: 07/01/13
Posts: 12
Loc: VT, USA
Last seen: 6 years, 9 months
Re: What NOT to do [Re: Inocuole]
    #23369993 - 06/22/16 01:36 PM (7 years, 7 months ago)

I'm dealing with this exact same issue! Had a fourth flush with 3 enormous fruits. 28g together when dried. But I too was on a trip, and came home to a goopy black mess.
I've been wondering what to do with the huge amount of contaminated concentrated spore solution I'm left with.


--------------------
Sleep is the greatest drug of all. The worst side effect is that when you wake up, it's tomorrow.
Naps are the solution.


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Offlinespacechildo
proletarians rise up
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Re: What NOT to do [Re: gravybrain]
    #23369997 - 06/22/16 01:40 PM (7 years, 7 months ago)

you can brush them off or simply dont give a fuck and eat them with spores.
if you want spores for cultivation take any other random cap and put it on foil and you'll have more than you'll ever need basically.


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OfflineCurious Shroomer
Noob
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Registered: 05/19/16
Posts: 118
Loc: Germany
Last seen: 1 year, 3 months
Re: What NOT to do [Re: SloppyJoseph]
    #23370054 - 06/22/16 02:03 PM (7 years, 7 months ago)

Quote:

SloppyJoseph said:
Quote:

the_r3dz said:

Sloppy you gotta show them what this turned into dude, that's the real star of this show




Yeah seriously. Now...

WHAT REALLY NOT TO DO



:rofl2:




WTH is that? 
And are you using an Icecream Box as a Tub there?


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InvisibleInocuole
Scalpel of Evil's Bane
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Re: What NOT to do [Re: Curious Shroomer]
    #23370061 - 06/22/16 02:06 PM (7 years, 7 months ago)

Read more.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


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