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shanesean023
NewGuy

Registered: 04/29/16
Posts: 117
Last seen: 6 years, 10 months
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Re: What NOT to do [Re: Kenetic]
#23368633 - 06/21/16 11:08 PM (7 years, 7 months ago) |
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Quote:
kenetic said: Hey dude you don't have to act like a pretentious asshole. You cleary don't know what you are talking about. For starters, how do you consider a fruiting chamber sterile? Your use of this word in this context makes it evident that you don't understand what this word actually means. There are much better ways to save a species than a dirty cotton swab. I'm not lazy, I'm simply smarter than you. I know not to waste my time with an uncontrolled variable when I can use proven methods.
Did I sat it was? I think it's pretty evident the sterile environment is the swab and everything you do with it. What are your better and proven methods since apparently no one has a proved way of using a sterile spore swab????
-------------------- Looking for a good Mexicana species, I have alot to trade. This account is strictly for role-playing purposes! Nothing that is said, posted, mentioned, or messaged is fact based and has never actually happened. Thanks for playing along.
Edited by shanesean023 (06/21/16 11:23 PM)
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NDStepp84
Stellar nuclear waste


Registered: 04/23/15
Posts: 4,956
Last seen: 1 month, 4 days
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--------------------
"I am free, no matter what rules surround me. If I find them tolerable, I tolerate them; if I find them too obnoxious, I break them. I am free because I know that I alone am morally responsible for everything I do. -Robert A. Heinlein
Links and teks ND's grow log and discussion Plant thread
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Gr0wer
always improving



Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX
Last seen: 5 years, 10 months
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Only issue i see is some black teeth....
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MMG
Artesano



Registered: 07/29/15
Posts: 547
Last seen: 2 years, 3 months
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Re: What NOT to do [Re: Gr0wer]
#23368770 - 06/21/16 11:54 PM (7 years, 7 months ago) |
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and fingers. dig the idea of making chocolate with mushrooms, I'm gonna try it soon.
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mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
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Quote:
shanesean023 said:
Quote:
kenetic said: Hey dude you don't have to act like a pretentious asshole. You cleary don't know what you are talking about. For starters, how do you consider a fruiting chamber sterile? Your use of this word in this context makes it evident that you don't understand what this word actually means. There are much better ways to save a species than a dirty cotton swab. I'm not lazy, I'm simply smarter than you. I know not to waste my time with an uncontrolled variable when I can use proven methods.
Did I sat it was? I think it's pretty evident the sterile environment is the swab and everything you do with it. What are your better and proven methods since apparently no one has a proved way of using a sterile spore swab????
dude... 
you obviously really dont know the meaning of the word sterile.. if you take a STERILE swab, and swab DIRTY spores off a mushroom cap in a NON-STERILE FC, then it isnt sterile anymore, is it?
and on another note, just because you posted you tube videos of congressmen saying something, doesnt mean it is absolute truth. You need actual factual evidence, and some congressman saying shit isnt that.
I cant tell if youre just a troll, or really this ignorant? But I can tell that you deserve the rating you have,
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
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SloppyJoseph
Non-sporalator


Registered: 12/08/15
Posts: 8,511
Loc:
Last seen: 3 years, 7 months
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Re: What NOT to do [Re: Gr0wer]
#23369323 - 06/22/16 08:28 AM (7 years, 7 months ago) |
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Quote:
Gr0wer said: Only issue i see is some black teeth....
Yeah, I guess it's not a big deal for some but I'm a bit of a perfectionist. I like my hard work to not look like the black death. And it was gonna be a pretty nice looking dub too, missed the kodak moment. And what else do we have on here except our pictures? :p
-------------------- AMU Q&A
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shanesean023
NewGuy

Registered: 04/29/16
Posts: 117
Last seen: 6 years, 10 months
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Quote:
mupetmower said:
Quote:
shanesean023 said:
Quote:
kenetic said: Hey dude you don't have to act like a pretentious asshole. You cleary don't know what you are talking about. For starters, how do you consider a fruiting chamber sterile? Your use of this word in this context makes it evident that you don't understand what this word actually means. There are much better ways to save a species than a dirty cotton swab. I'm not lazy, I'm simply smarter than you. I know not to waste my time with an uncontrolled variable when I can use proven methods.
Did I sat it was? I think it's pretty evident the sterile environment is the swab and everything you do with it. What are your better and proven methods since apparently no one has a proved way of using a sterile spore swab????
dude... 
you obviously really dont know the meaning of the word sterile.. if you take a STERILE swab, and swab DIRTY spores off a mushroom cap in a NON-STERILE FC, then it isnt sterile anymore, is it?
and on another note, just because you posted you tube videos of congressmen saying something, doesnt mean it is absolute truth. You need actual factual evidence, and some congressman saying shit isnt that.
I cant tell if youre just a troll, or really this ignorant? But I can tell that you deserve the rating you have,
You obviously don't know shit if I have to spoon feed you every lil damn thing. OK take a box a q tips place in GB spray with oust wait 5 minutes and what do you have moron
-------------------- Looking for a good Mexicana species, I have alot to trade. This account is strictly for role-playing purposes! Nothing that is said, posted, mentioned, or messaged is fact based and has never actually happened. Thanks for playing along.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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swabs without spores on them arent worth much to me, spores aren't clean are what people are saying so once you swab a sterile swab on a cap or print its no longer sterile 
agar is great for making sure you have a clean inoculant, not daring to store the culture in fear of a power-out doesnt take that away!
oh and btw, lysoling or ousting a q-tip doesnt make it sterile, PC'ing it does.
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shanesean023
NewGuy

Registered: 04/29/16
Posts: 117
Last seen: 6 years, 10 months
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Quote:
spacechildo said: swabs without spores on them arent worth much to me, spores aren't clean are what people are saying so once you swab a sterile swab on a cap or print its no longer sterile 
agar is great for making sure you have a clean inoculant, not daring to store the culture in fear of a power-out doesnt take that away!
oh and btw, lysoling or ousting a q-tip doesnt make it sterile, PC'ing it does.
Exactly that's why I said to transfer to the LC to agar. I said do x transfers on agar to clean up your mycelium. An keep the LC as a almost limitless supply of said strain and a safe backup that can be, will be, and is cleaned in the end
-------------------- Looking for a good Mexicana species, I have alot to trade. This account is strictly for role-playing purposes! Nothing that is said, posted, mentioned, or messaged is fact based and has never actually happened. Thanks for playing along.
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shanesean023
NewGuy

Registered: 04/29/16
Posts: 117
Last seen: 6 years, 10 months
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Everytime I PC q tips they swell big af or or become flimsy af. I'll take my chances with .001% germs and bacteria.
-------------------- Looking for a good Mexicana species, I have alot to trade. This account is strictly for role-playing purposes! Nothing that is said, posted, mentioned, or messaged is fact based and has never actually happened. Thanks for playing along.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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from LC to agar is the wrong way, clean a culture up on agar and then throw wedges in grain jars or LC, and a small transfer to a slant if its a clone or culture you wanna keep.
if you dont sterilize your Q-tip you shouldn't use it, flame a loop instead.
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shanesean023
NewGuy

Registered: 04/29/16
Posts: 117
Last seen: 6 years, 10 months
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That's a great way. Wouldn't you still want to clean that LC on agar everytime before you go to grain or GLC? So you'd be starting right back at LC to agar to GLC.But yes agar to LC would definitely be best to start
-------------------- Looking for a good Mexicana species, I have alot to trade. This account is strictly for role-playing purposes! Nothing that is said, posted, mentioned, or messaged is fact based and has never actually happened. Thanks for playing along.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Actual Q tips can be sterilized pretty easily but I use the pre-sterile wooden ones in individual sleeves. I also only take spores from sterile invitro fruits.
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LocN9ne
ɢᄋᄋd ԲᄋЯ ᄁᄋȚᅢΙᄁɢ ᄂᄋ₩ᄂΙԲᄐ



Registered: 04/17/15
Posts: 7,076
Loc: to the brain
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Quote:
shanesean023 said: Everytime I PC q tips they swell big af or or become flimsy af. I'll take my chances with .001% germs and bacteria.
That's just the amount needed to Fuck ya... Weird how that works out... You need to individually wrap the qtips in foil, then put them in a jar and PC them... They should still be dry after the PC cycle man...
--------------------
Q&A US vs. THEM The more I learn, the less I know.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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Quote:
shanesean023 said: That's a great way. Wouldn't you still want to clean that LC on agar everytime before you go to grain or GLC? So you'd be starting right back at LC to agar to GLC.But yes agar to LC would definitely be best to start
no, not at all. a dirty LC is trash, its just too much work cleaning it up on agar. and LCs are used to noc up grains, there's not much point in going to LC if you're just gonna use it to start an agar wedge. You're just going in a loop that makes no sense, where does the GLC come into play??
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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LC to agar to GLC. What a waste of time..
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gravybrain
Stranger

Registered: 07/01/13
Posts: 12
Loc: VT, USA
Last seen: 6 years, 9 months
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Re: What NOT to do [Re: Inocuole]
#23369993 - 06/22/16 01:36 PM (7 years, 7 months ago) |
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I'm dealing with this exact same issue! Had a fourth flush with 3 enormous fruits. 28g together when dried. But I too was on a trip, and came home to a goopy black mess. I've been wondering what to do with the huge amount of contaminated concentrated spore solution I'm left with.
-------------------- Sleep is the greatest drug of all. The worst side effect is that when you wake up, it's tomorrow. Naps are the solution.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
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you can brush them off or simply dont give a fuck and eat them with spores. if you want spores for cultivation take any other random cap and put it on foil and you'll have more than you'll ever need basically.
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Curious Shroomer
Noob


Registered: 05/19/16
Posts: 118
Loc: Germany
Last seen: 1 year, 3 months
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Quote:
SloppyJoseph said:
Quote:
the_r3dz said:
Sloppy you gotta show them what this turned into dude, that's the real star of this show
Yeah seriously. Now...
WHAT REALLY NOT TO DO


WTH is that? And are you using an Icecream Box as a Tub there?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Read more.
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