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OfflineDeadPhan
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Registered: 05/05/04
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Pretty sure my poly fil filters are reason for my contams
    #23350433 - 06/16/16 10:06 AM (7 years, 7 months ago)

Ive been back and forth now for a couple years on contam sources. Unfortunately it's been kind of hard to consistently pinpoint as my work areas have changed many times. Ive been in cool basements, hot garages, tents, etc. For a while I thought could be my flowhood.  In the winter months I have pretty much 100%.  For the first couple years I lived with my ex I had the garage. In the winter months I would have 100 percent success. I would do all my innocs and sterilization out there. I would store my jars in the house at room temp. In the summer in the same spot I would get aloy more contams. Initially I assumed becUse of the heat and more shit in the air that maybe flowhood wasnt as effective.  Which is probaly partly true. However, when I would work in front of hood and store somewhere inside mostly success. Last summer, I stored a bunch of sterilized jars before innoculating in a hot barn and they all got contamed. I just recently did I batch of 14 jars in a small enclosure and everything was moving along nicely. Until recently when I started noticing contams at the top of the jars. I had yo store them outside in a shed. I feel the polyfil lets in contams. Ive tested the flowhood in the past with unaltered lids on half pint jars with agar and no contams. I pack my poly hella tight and my sterilization tech is like clockwork. Ive had many good runs. I have issues in the summer with having.to store outside. My next batch I just ordered syntetic filter discs for. Im starting on agar and will try to narrow down if the poly is the source of my contams. I find them effective filters but with a high spore load I think they manage ti breathe shit through.


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Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Pretty sure my poly fil filters are reason for my contams [Re: DeadPhan]
    #23350484 - 06/16/16 10:22 AM (7 years, 7 months ago)

I only used it twice, and both times I got a contaminate. Though I wasn't doing traditional grains. I think it was either whole brown rice or a pf tek that way 1/8th empty for inoculation.


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Invisiblemaddchef
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Registered: 09/04/09
Posts: 5,602
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Re: Pretty sure my poly fil filters are reason for my contams [Re: DeadPhan]
    #23350488 - 06/16/16 10:23 AM (7 years, 7 months ago)

Yeah I went through the same thing. Wasn't my problem.

If you leave a blank jar and it contams right on top then yes it's your filters. If it's elsewhere then it's not. Be sure not to shake or otherwise distribute your grains.

Are you seeing mold contams? Are you seeing them before spawning?


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineYaMoonSun
The Double Standard


Registered: 10/23/14
Posts: 3,967
Loc: NY
Last seen: 5 months, 11 days
Re: Pretty sure my poly fil filters are reason for my contams [Re: YaMoonSun]
    #23350504 - 06/16/16 10:27 AM (7 years, 7 months ago)

In hindsight, my LC was contaminated with bacteria and trich


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OfflineDeadPhan
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Registered: 05/05/04
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Re: Pretty sure my poly fil filters are reason for my contams [Re: maddchef]
    #23350584 - 06/16/16 10:47 AM (7 years, 7 months ago)

Mostly trich but some mold. And yea. I hadnt shook any of the jars. Healthy myc at innoc points and.eventually contam at odd points on top. I had a bunch last simmer I had not gotten.around to innoculating for a lil over a.week and found them all contamed from the top down. My polyfil is very tight. I just think storing.them.outside they somehow get contams.slippin through. This mext batch will be started on.agar and transfered to jars with sfd. Ill do some control experiments with non inoculated pily and sfd. And innoc poly and sfd. See what happens. But fairly certain thats the cause


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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Invisiblemaddchef
Vaginal escape artist
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Registered: 09/04/09
Posts: 5,602
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Re: Pretty sure my poly fil filters are reason for my contams [Re: DeadPhan]
    #23350627 - 06/16/16 10:59 AM (7 years, 7 months ago)

That'll tell you.

Leave a few blanks of poly and a few blanks of sfd jars. If one contams and not the other you will have your answer.


Poly works but it is an imperfect filter since our holes will never be a true circle and when we stuff it will not expand with the same force in all directions.

That being said I don't like the cellulose sfd's either. Although I use them if I continue to use jars I will buy the actual sfd's


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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OfflineDeadPhan
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Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
Re: Pretty sure my poly fil filters are reason for my contams [Re: maddchef]
    #23350682 - 06/16/16 11:14 AM (7 years, 7 months ago)

Yea. I just decided to buy 10 125mm discs from fungi perfecti. Im just gonna cut out circles for my 3/8 inch holes on my lids and glue on with RTV silicone. I gather I should get about 100 lids done in that fashion. Whatda ya think?


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


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OfflineDeadPhan
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Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
Re: Pretty sure my poly fil filters are reason for my contams [Re: DeadPhan]
    #23350686 - 06/16/16 11:17 AM (7 years, 7 months ago)

Worst part of this is that I was hoping.to have alot of square footage of outdoor beds by now. It's looking like I wont be laying beds for a couple weeks at best now. Hopefully we have a good late summer for a fall harvest


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


Extras: Filter Print Post Top
OfflineDeadPhan
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Registered: 05/05/04
Posts: 5,260
Last seen: 4 years, 2 months
Re: Pretty sure my poly fil filters are reason for my contams [Re: DeadPhan]
    #23350700 - 06/16/16 11:23 AM (7 years, 7 months ago)

I have 8 jars that still hold promise. If just one of them is clean I can g2g and still be on a decent schedule. But ima be innocing some agar in the mext couple days either way.


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
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Registered: 01/24/13
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Re: Pretty sure my poly fil filters are reason for my contams [Re: DeadPhan]
    #23350712 - 06/16/16 11:26 AM (7 years, 7 months ago)

are you sure its trich and not penicillium? those spores are tiny and can be a bitch.
try and store your colonizing grain jars in a tub or something that helps spore load off the filter.


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OfflineDeadPhan
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Registered: 05/05/04
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Re: Pretty sure my poly fil filters are reason for my contams [Re: spacechildo]
    #23350727 - 06/16/16 11:28 AM (7 years, 7 months ago)

Just assumed trich. Is pennicilium green?


--------------------
 


Big Gulps!  Alright!  Well,  See ya later!
And if i claim to be a wise man, well, it surely means that i dont know!


Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
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Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Re: Pretty sure my poly fil filters are reason for my contams [Re: DeadPhan]
    #23350759 - 06/16/16 11:38 AM (7 years, 7 months ago)

yeah its a slightly different kind of green, more blue-ish to my eyes at least and a different texture.

there should be some good pics in kizzle's sticky in the contam forums.


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InvisiblebodhisattaMDiscordReddit
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Re: Pretty sure my poly fil filters are reason for my contams [Re: spacechildo]
    #23351218 - 06/16/16 02:07 PM (7 years, 7 months ago)

Name brand polyfil (one L) works better IMO. I used poly for over a year no problem. You really have to stuff it so tight as to almost tear the hole open more.


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Invisiblemaddchef
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Registered: 09/04/09
Posts: 5,602
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Re: Pretty sure my poly fil filters are reason for my contams [Re: bodhisatta]
    #23351313 - 06/16/16 02:36 PM (7 years, 7 months ago)

lol @ name brand poly


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery
Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Pretty sure my poly fil filters are reason for my contams [Re: maddchef]
    #23351474 - 06/16/16 03:25 PM (7 years, 7 months ago)

Polyfil (TM)
But it's better than Walmart brand or shit out of pillows


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