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Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

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Invisiblebastard4life
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Registered: 03/12/11
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isolating on petri dishes
    #23350188 - 06/16/16 08:29 AM (7 years, 7 months ago)

Im doing my first set of plates in a few days. They are falcon disposable petri 90x15mm. I want to know from a print or syringe how many petris to get an isolate. I'd like to start 2 cubes together. Is reserving 9 petris a piece overkill or just about right. I'm using a glovebox with jars now but I PC the jars and agar together move to glovebox and pour it because I was doing no pour in jars but always had excess water.  I don't think I can get away with 3 cube isolates of different varieties with only 20 dishes. Oh and for master slants im getting 20 16x100mm cultubes with white autoclavable and antibacterial caps. I figured antibacterial and PC safe is a great idea. I also have parafilm. Its starting to look a lot like agar time for real.


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Invisiblemupetmower
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Re: isolating on petri dishes [Re: bastard4life]
    #23350226 - 06/16/16 08:44 AM (7 years, 7 months ago)

it will probably take more than 9 plates to transfers and get an isolate.


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Edited by mupetmower (06/16/16 08:45 AM)


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Invisiblebastard4life
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Re: isolating on petri dishes [Re: mupetmower]
    #23350251 - 06/16/16 08:55 AM (7 years, 7 months ago)

OK so get a sleeve of 20 per each print syringe being used. No prob. I'll just order one more.


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