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blackout


Registered: 07/16/00
Posts: 5,266
Last seen: 2 months, 23 days
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Extra clean spore syringes
#23347117 - 06/15/16 12:11 PM (7 years, 7 months ago) |
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Has any vendor ever sold or thought of selling, or indeed right now selling extra clean spore syringes, the extra cleanliness could command a higher price. These days almost everyone seems to warn against using spores direct in LCs, I have done it numerous times and have had no issues from one vendor (for cubensis) but have from others. Growing & spore printing does not have to happen in open air and there may be other secretive methods sellers already use. The syringes could also be extremely light, I read PF used to use agar water to keep to spores in his light syringes dispersed evenly.
I guess some sellers be put off as it could be seen as admitting their regular syringes are not 100% clean, but is commonly said they are not.
This would be hugely encouraging to new growers thinking of starting out. They may not want to state/guarantee they are 100% clean but just that they have gone the extra mile and not scared to have them tested out in LCs.
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Ryche Hawk
A Messenger



Registered: 03/01/01
Posts: 2,112
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Re: Extra clean spore syringes [Re: blackout]
#23350460 - 06/16/16 10:15 AM (7 years, 7 months ago) |
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I've used my syringes numerous times in LC's without problems but the solution should go on grain afterward, not agar.
Paul Stamets himself teaches spores will always have trace amounts of bacteria. When making spore syringes the water can help magnify that bacteria, but when inoculated in grain the mycelium easily out completes that bacteria so you don't see it. But when put on agar, the bacteria can spread faster then the mycelium. This is why I don't recommend spore syringe on agar, only use spore prints for agar and sector out any bacteria. Although I have seen times where no bacteria shows on agar from spore syringes I've just as many times it does. But those syringes that showed bacteria on agar when used on grain showed nothing but good healthy mycelium growing.
-------------------- -Peace- High Quality MUSHROOM SPORES and CULTURES for microscopy at www.muShrooms.com
muShrooms.com is the new web site of www.thehawkseye.com
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MRCA
Tyroler Gluckspilze



Registered: 06/23/08
Posts: 160
Loc: Europe / Austria / Tyrol ...
Last seen: 5 years, 4 months
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Re: Extra clean spore syringes [Re: Ryche Hawk]
#23353628 - 06/17/16 05:00 AM (7 years, 7 months ago) |
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We can guarantee for the quality, pureness and cleanness of our spore syringes. The production is made in class 100 clean room environment. All syringes are tested on agar plates before they are sold. Only if the spores germinate fast and without any trace of contamination we put them on shelve. Our mushrooms are grown in finest organic quality, we only use raw materials from organic farming, mainly from local suppliers.
To find out more, visit our web page: gluckspilze.com gluckspilze.com/Spores-for-microscopy
Edited by MRCA (06/17/16 05:01 AM)
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Yerow
Stranger



Registered: 09/22/14
Posts: 1,206
Last seen: 2 years, 7 months
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Re: Extra clean spore syringes [Re: MRCA]
#23355320 - 06/17/16 04:18 PM (7 years, 7 months ago) |
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I can easily vouch for MRCA, never had a issue. And had many orders with them
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Extra clean spore syringes [Re: Yerow]
#23387640 - 06/27/16 12:48 PM (7 years, 6 months ago) |
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If you do a proper streak plate from a syringe even with a dirty syringe you should be able to salvage. Honestly never had a problem much with any vendors syringe "microscopically"
At the end of the day there's big fuss about everything but the personal responsibility to be able to use what you get. 10$ to get started with spores for identification purposes only is pocket change
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