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OfflineLogicaL ChaosM
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Success with Isopropol
    #23347003 - 06/15/16 11:39 AM (7 years, 8 months ago)

So i just checked on my week old pf jrs, all have signs of growth and no signs of infections.

I used only isopropol wipe downs of my needle inbetween each injection.

Am i just getting lucky or is isopropol an effective way to clean a needle?


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Invisiblemupetmower
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Re: Success with Isopropol [Re: LogicaL Chaos]
    #23347027 - 06/15/16 11:44 AM (7 years, 8 months ago)

just lucky. i did the same with my first batch of cakes, and all 6 came out fine. but its just luck.


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-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.

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InvisibleInocuole
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Re: Success with Isopropol [Re: mupetmower]
    #23347034 - 06/15/16 11:47 AM (7 years, 8 months ago)

Is there any major factor preventing you from flaming the needle?  I enjoy flaming my tools since I started using a torch to do it.  Real clean, real fast.  Isopropyl barely has a place in my workflow anymore.


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Invisiblemupetmower
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Re: Success with Isopropol [Re: Inocuole]
    #23347041 - 06/15/16 11:49 AM (7 years, 8 months ago)

i think flaming it pretty fun, too. because you get to use a damn blow torch!!

:kingtard:


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.

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Invisiblemaddchef
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Re: Success with Isopropol [Re: mupetmower]
    #23347050 - 06/15/16 11:52 AM (7 years, 8 months ago)

flamers


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In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

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OfflineLittleDaddy
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Re: Success with Isopropol [Re: maddchef]
    #23347072 - 06/15/16 11:57 AM (7 years, 8 months ago)

isopropyl works fine... it will kill everything in high enough concentrations, but once it evaporates, you're vulnerable again (70%+ helps with this because the water prevents it from evaporating that fast).

when working in the biohood, we spray everything down just with 70% or 100% ethanol (comparable to isopropyl) before placing in the hood and the infections we do see are typically related to technique or spore outbreaks (somewhat resistant to alcohol).


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The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.

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InvisibleInocuole
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Re: Success with Isopropol [Re: LittleDaddy]
    #23347089 - 06/15/16 12:02 PM (7 years, 8 months ago)

Quote:

LittleDaddy said:
isopropyl works fine... it will kill everything in high enough concentrations




Except mold spores, but those are probably fine right?  Doesn't isopropyl also kill more things in LOWER concentrations?  It's pretty well established that 70% is more effective than 90%.


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InvisiblebodhisattaMDiscordReddit
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Re: Success with Isopropol [Re: LittleDaddy]
    #23347098 - 06/15/16 12:04 PM (7 years, 8 months ago)

100% ethanol is very expensive. 95% ethanol is way cheaper. 70% ethanol works better as a sanitizer than either just the same way 70% ISO works better than higher concentration.

Neither kill everything, they both require a contact time of a few minutes to do their 99.99% kill.

You just get lucky using alcohol on needles

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OfflineLittleDaddy
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Re: Success with Isopropol [Re: Inocuole]
    #23347118 - 06/15/16 12:11 PM (7 years, 8 months ago)

Concentration wasn't right, I meant quantity. It will kill nearly all spores in high enough quantities


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.

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InvisiblePastywhyteMDiscord
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Re: Success with Isopropol [Re: LittleDaddy]
    #23347189 - 06/15/16 12:38 PM (7 years, 8 months ago)

Quote:

LittleDaddy said:
Concentration wasn't right, I meant quantity. It will kill nearly all spores in high enough quantities




No it won't. There have been to my knowledge cases of massive trich outbreaks on commercial farms because of that exact assumption. It can kill 99.999%. So in the case of you having 1 billion CFU's on your hands, soaking them in iso alcohol for a few minutes will result in 10000 CFU's surviving. See the problem?

Flame kills everything everytime. Why mess with perfection? Some soot? Not worth it IMO.

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OfflineLittleDaddy
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Re: Success with Isopropol [Re: Pastywhyte]
    #23347337 - 06/15/16 01:24 PM (7 years, 8 months ago)

I agree, flame is better, but isopropyl has effective success.

On commercial scale, it would be dumb to use isopropyl when you can flame. I don't think 10^9 CFU's of possible contamination will be on a syringe. I'm talking about personal cultivation. I've yet to have contamination from it. I'm not going to say it won't happen, but if I lose one jar from it, so be it. Personally, I flame sterilize before first inoc and then isp the subsequent ones.


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The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.

Edited by LittleDaddy (06/15/16 01:31 PM)

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Invisiblemaddchef
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Re: Success with Isopropol [Re: LittleDaddy]
    #23347351 - 06/15/16 01:27 PM (7 years, 8 months ago)

We use 70% instead of 90 or 100 for mainly the reason the water helps penetrate cell membranes or something to that effect. Osmosis or some other big word.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:

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OfflineSloppyJoseph
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Re: Success with Isopropol [Re: maddchef]
    #23347362 - 06/15/16 01:32 PM (7 years, 8 months ago)

Quote:

maddchef said:
We use 70% instead of 90 or 100 for mainly the reason the water helps penetrate cell membranes or something to that effect. Osmosis or some other big word.



Coagulation


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OfflineLittleDaddy
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Re: Success with Isopropol [Re: SloppyJoseph]
    #23347374 - 06/15/16 01:35 PM (7 years, 8 months ago)

So using 70% doesn't have to do with the added water raising vaporization E?

(Edit: just read more about it, I stand corrected - I apologize for the misinformation about mechanism of action being that it lowers vaporization energy)


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.

Edited by LittleDaddy (06/15/16 01:41 PM)

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Invisiblenatedawgnow
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Re: Success with Isopropol [Re: maddchef]
    #23347389 - 06/15/16 01:38 PM (7 years, 8 months ago)

91 has plenty of water for osmosis and coagulation of cell membrane but too much alcohol for it to be
effective because of how fast it evaporates. Make a streak with a cotton
swab on a piece of glass with each and you'll see it clearly.

70 works better because
the water contents slows evaporation enough to let it do its job seeing as
it needs a couple minute soak time for sanitation to occur.


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Invisiblemupetmower
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Re: Success with Isopropol [Re: natedawgnow]
    #23347398 - 06/15/16 01:40 PM (7 years, 8 months ago)

even with all this(which has been said in threads a 100 times), it still will NOT sterilize. flame is what you need to sterilize with. if you wipe it with ISO after the flame, then the previous flaming was pointless. just flame your needle/blade/loop.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.

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OfflineLogicaL ChaosM
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Re: Success with Isopropol [Re: Inocuole]
    #23347438 - 06/15/16 01:49 PM (7 years, 8 months ago)

Quote:

Inocuole said:
Is there any major factor preventing you from flaming the needle?  I enjoy flaming my tools since I started using a torch to do it.  Real clean, real fast.  Isopropyl barely has a place in my workflow anymore.




I dont like flaming cause i dont like the idea of cooking my spores in the needle. spores are precious!

Plus, the soot looks gross.

I like a clean looking needle, so i used 99% iso. i guess im gambling it, but it seems my gamble worked.

Whats ironic, is thta im a total pyro, yet i dont like flaming needles :shrug:

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InvisibleInocuole
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Re: Success with Isopropol [Re: LogicaL Chaos]
    #23347461 - 06/15/16 01:53 PM (7 years, 8 months ago)

If you use a torch or alcohol lamp there's no soot and you have absolute sterility.  You only need a few spores to do the job, there's millions or billions in the syringe.  Spores just aren't that precious to me I guess. :shrug:  I don't ever put spores into syringes though, and I certainly don't buy them all the time, so it's rare that I work with them at all.  So take that for what it is.  But I personally like flaming needles.  With a butane or propane torch it happpens in less than a second and it cools off pretty quick too.

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Invisiblemupetmower
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Re: Success with Isopropol [Re: LogicaL Chaos]
    #23347470 - 06/15/16 01:55 PM (7 years, 8 months ago)

you are killing a drop or two of spores... and there is very little soot if you use a torch. it really is gambling, and will definitely fail more often than flaming. if i was getting contams, that would be the first and obvious cause.


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.

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InvisibleInocuole
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Re: Success with Isopropol [Re: mupetmower]
    #23347478 - 06/15/16 01:56 PM (7 years, 8 months ago)

Nobody bothered to correct me wanting to shoot spores through an isopropyl soaked sponge when I first made this account in 2011.  Just be glad people here care now. :lol:


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