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Offlinegremtu
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Myc ate tam in dish. Still good to xfer?
    #23341332 - 06/13/16 09:40 PM (7 years, 7 months ago)

Hello. I have a few dishes that had a little bacteria in them. I sat them up and forgot about them and they fully colonized the plates consuming the bacteria. Is the mycelium still alright to transfer to a new dish?


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InvisibleLocN9ne
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Re: Myc ate tam in dish. Still good to xfer? [Re: gremtu]
    #23341364 - 06/13/16 09:48 PM (7 years, 7 months ago)

I guarantee you the myc did not eat the bacteria... And it is still there... But that is kinda what agar is for... You wanna transfer away from contains as soon as you can.


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Offlinegremtu
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Re: Myc ate tam in dish. Still good to xfer? [Re: LocN9ne]
    #23341376 - 06/13/16 09:52 PM (7 years, 7 months ago)

Alright, so it will be alright if i take a small wedge and transfer it as usual then?

Ususally I transfer as soon as i see bacteria but this just sat to long and overgrew the spots.


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Edited by gremtu (06/13/16 09:53 PM)


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InvisibleLocN9ne
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Re: Myc ate tam in dish. Still good to xfer? [Re: gremtu]
    #23341384 - 06/13/16 09:54 PM (7 years, 7 months ago)

Yup... Then transfer again as soon as you see decent growth... If you can't get away from the bacteria in a few transfers look into "agar sandwich"ing that shit.


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Offlinegremtu
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Re: Myc ate tam in dish. Still good to xfer? [Re: LocN9ne]
    #23341400 - 06/13/16 09:58 PM (7 years, 7 months ago)

So i was right then.... I had someone that thinks they know everything yesterday tell me that if i transferred it, that it would still be tammed on a biological level or some shit... I figured that it was just bullshit because ive never had a problem with it. you confirmed. thank you brother.


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InvisiblebodhisattaMDiscordReddit
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Re: Myc ate tam in dish. Still good to xfer? [Re: gremtu]
    #23341610 - 06/13/16 10:54 PM (7 years, 7 months ago)

You ideally want to transfer before the mycelium grows over. Now when you transfer you'll bring some along and then have to transfer again to get away from it. Not the end of the world but you'll have to do less transfers to get away from contamination if you don't let it get out of hand. Mycelium doesn't eat the contamination its still there.


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OfflineMycoLoopology
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Re: Myc ate tam in dish. Still good to xfer? [Re: bodhisatta]
    #23341615 - 06/13/16 10:55 PM (7 years, 7 months ago)

well i just learned something new today. i never realized that was one of the benefits of agar. now im REALLY considering going back to using agar to grain instead of purely G2G...

not to mention how much fun it is to work with petri dishes hehe


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Re: Myc ate tam in dish. Still good to xfer? [Re: bodhisatta]
    #23343191 - 06/14/16 12:10 PM (7 years, 7 months ago)

Quote:

gremtu said:
So i was right then.... I had someone that thinks they know everything yesterday tell me that if i transferred it, that it would still be tammed on a biological level or some shit... I figured that it was just bullshit because ive never had a problem with it. you confirmed. thank you brother.




Quote:

Trusted Cultivator said:
You ideally want to transfer before the mycelium grows over. Now when you transfer you'll bring some along and then have to transfer again to get away from it. Not the end of the world but you'll have to do less transfers to get away from contamination if you don't let it get out of hand. Mycelium doesn't eat the contamination its still there.




:whathesaid:

That's basically what's happening and it's not bullshit, bacteria can get such a hold on mycelium that they're stuck until you do the agar sandwich, and then if it's something else, mold or whatever, the agar sandwich may not even work.

That's why you transfer before the mycelium comes anywhere near the contamination, not wait until its' run it over, then transfer both.


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