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Offlinecandela
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Registered: 09/04/15
Posts: 281
Last seen: 2 years, 6 months
Rookie Agar and Bacteria Question
    #23341246 - 06/13/16 09:22 PM (7 years, 7 months ago)

My Agar Definately is contaminated, Ive already learned how to identify that milky look is bacteria :crazy2:

My understanding is that I can cut and transfer a wedge from the edge, where there is rhizmorphic, and keep transfering wedge edges of rhizmorphic to new plates, until I have a full plate of nice clean looking rhizmorphic mycellium, correct my if I am wrong please.

Should I be concerned that I will just keep transfering bacteria from plate to plate as well, if my first plate was full of milky bacteria?


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InvisibleKush_Zombie
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Registered: 10/22/14
Posts: 4,793
Re: Rookie Agar and Bacteria Question [Re: candela]
    #23341278 - 06/13/16 09:28 PM (7 years, 7 months ago)

Yep, do transfers. I don't even do the edge of the mycelium sometimes, sometimes I just get the middle or wherever I feel like really. I turn one plate into about 4 more when I transfer (flaming between each transfer and have good success.

Also are you starting from spore print? If so then the bacteria is pretty normal. Agar is made so that you can identify the mycelium from the contains easily and make transfers until you're working with cultures you find satisfactory.

Just go for it man. You'll probably surprise yourself with how much better it looks after a transfer of your sab conditions are up to par.


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How to get started in bulk:
Presto 23-Quart Pressure Cooker
BOD's Simple as FUCK Still Air Box
PastyWhyte's Easy Agar Tek
Munchauzen's Cultivation Video Series
How EvilMushroom666 Prepares His Grains (I use jars with Synthetic Filter Discs)
What is G2G? (Grain-to-Grain)
Damion5050's Coir Tek (I use 5.5 - 6 quarts of water instead of 4. Also ignore step 13 and ignore the monotub completely. The only purpose of this tek is to show you how to make a simple substrate. I also add gypsum to it but not necessary)
Spitball's Monotub Tek (A liner isn't necessary but is useful)
Use 6500k lights throughout the whole process. When you wake up, turn the light on. When you go to sleep turn the light off. It's as simple as that.


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