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Invisiblesakura
Aussie Expat

Registered: 02/08/04
Posts: 592
Loc: Japan
Would this work???
    #2333540 - 02/14/04 05:40 AM (14 years, 3 months ago)

If someone were to soak, boil and then drain well a grain mix (say birdseed for the sake of discussion).

Leave for 30 hours + as per fractional sterilisation...

Sterilise in jars in the oven (250Deg 90 mins say... Yes I know it dries the medium... read on).

Not just innoculate, but actually hydrate the resulting medium with a fairly mature karo/honey tek medium with a rather largish syringe... (poke sterile needle though the hole, into the cake and move it around, breaking up the cake, squeezing all the way and trying to distribute the mycelium through the mixture)

Would you get shrooms or smelly gunk?


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OfflineRoseM
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Registered: 09/24/03
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Re: Would this work??? [Re: sakura]
    #2333551 - 02/14/04 05:46 AM (14 years, 3 months ago)

I'm sure people will doubt this will work. But, it is pretty original. Try it if you are still curious after the answers you get. You may be on to something new.

My gut says no. WBS needs a pc from all I've heard.


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Fiddlesticks.



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Offlinekilgore_trout
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Registered: 10/18/03
Posts: 1,607
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Re: Would this work??? [Re: Rose]
    #2333632 - 02/14/04 07:40 AM (14 years, 3 months ago)

ive heard the same.

boiling=200degrees
boiling at 15 psi(pressure cooking)=285degrees

if youre going to do it, dont do it at below pressure cooking temp.
id go 285-300 depending on what you can set your oven to.

ive also heard of using a covered container and a water bath in the oven and adding water throughout cooking as it evaporates to combat drying. this however, is unpractical for any real amount of jars and your proposition may be as well since it sounds like youre saying you want to use one syringe per jar. but if youve got the syringes, fuck it. i say try it either way. you could also do something to limit gas exchange so they dont dry out as fast, but that could be a problem later when you need gas exchange. fuck with the idea and think about how to fix every problem as best you can. maybe come up with a removable lid cover with a small hole that would limit drying.

good luck, and try it with one jar first so if its shit you dont have a lot of shit.


--------------------
"I didnt fight a secret war in nicaragua so you could walk these streets of freedom bad-mouthing lady america in your damn mirrored sunglasses."


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InvisiblePrisoner#1
Even Dumber ThanAdvertized!
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Registered: 01/22/03
Posts: 193,665
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Re: Would this work??? [Re: sakura]
    #2333705 - 02/14/04 09:22 AM (14 years, 3 months ago)

Quote:

sakura said:
Sterilise in jars in the oven (250Deg 90 mins say... Yes I know it dries the medium..





soak the grain and do not drain it as you usualy would, just give it a couple minutes to drain, fill your jars, cover with foil and pop it in an oven ar 400F for 45-60 minutes and you may find success... rubbers and plastics dont hold up well under those sonditions tho


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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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