|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: Pastywhyte's Easy Agar Tek [Re: deagle5]
#23315463 - 06/06/16 07:15 PM (7 years, 8 months ago) |
|
|
Flied lice, anyone?
|
esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 12 minutes, 6 seconds
|
Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#23315584 - 06/06/16 07:42 PM (7 years, 8 months ago) |
|
|
Amazing guide, as you suggested me I'll start with PDA agar tomorrow and my question is: how much water it's supposed to be in my pc ?? also can I PC the jars stacked over other jars??
sorry but I'm scared to fail again
--------------------
things does not exist, everything is a process, so we proceed
|
Myko Fanatikos
Rando



Registered: 02/18/16
Posts: 283
Loc: Earth
Last seen: 10 months, 26 days
|
Re: Pastywhyte's Easy Agar Tek [Re: esse_jeremy]
#23315684 - 06/06/16 08:11 PM (7 years, 8 months ago) |
|
|
Enough water so it doesn't evaporate and melt everything into a pp5 agar puddle lol.
You can stack them, I do. I use the same little midget pp5 glad mini rounds this tek uses and I put the water level about 3/4 way to the top of the first set of rounds.
--------------------

|
Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
|
|
Quote:
Supalemonhaze said: Flied lice, anyone?
U Rike Fry Rye?
|
KinokoKomodo
マッシュルーム



Registered: 05/22/16
Posts: 776
Loc: North Florida
Last seen: 2 years, 6 months
|
Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
#23317021 - 06/07/16 04:44 AM (7 years, 8 months ago) |
|
|
Quote:
Inocuole said: Out of all the things in the world that are racist, finding engrish funny really isn't one of them, engrish is fucking hilarious.
for sure! my user title is actually the japanese pronunciation mushroom, pronounced maash-room-u. the japanese language doesn't have a whole lot of sounds the english language and similar romance languages share. There is no 'muh', only 'moo' which doesn't fit and 'maa.' 'mushroom' for example, and most english words actually, intentionally get a -u sound on the end to define it as an english word because they borrow so many of them. so they get it as close as possible with what they have...and you're left with maash-room-u lol
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
#23317029 - 06/07/16 04:54 AM (7 years, 8 months ago) |
|
|
Quote:
Inocuole said:
Quote:
Supalemonhaze said: Flied lice, anyone?
U Rike Fry Rye?
Hao long wai ting? Ai wan fry rye nao!
|
esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 12 minutes, 6 seconds
|
|
ok I Innoc. six agar plates with MS! now how much they will take to germinate?? because I saw ppl that have the myc after 3 days, other after 7, the avg is 7 to 14 but other said that after a month is still normal to have no germination on agar...what's your opinion?? I thought that agar will speed up the germination compared to MS>>Wbs grain....
--------------------
things does not exist, everything is a process, so we proceed
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
|
Re: Pastywhyte's Easy Agar Tek [Re: esse_jeremy]
#23324975 - 06/09/16 08:03 AM (7 years, 8 months ago) |
|
|
It's not that it speeds it up. It's that once you do agar for a while you will have live culture already on hand. So when your ready to go you just pull a plate and go.
The big benefit is being able to see a clean colony right off the bat.
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: Pastywhyte's Easy Agar Tek [Re: esse_jeremy]
#23325024 - 06/09/16 08:20 AM (7 years, 8 months ago) |
|
|
Quote:
esse_jeremy said: ok I Innoc. six agar plates with MS! now how much they will take to germinate?? because I saw ppl that have the myc after 3 days, other after 7, the avg is 7 to 14 but other said that after a month is still normal to have no germination on agar...what's your opinion?? I thought that agar will speed up the germination compared to MS>>Wbs grain....
Agar germination with spore syringes/solutions is usually more difficult than prints. The two spore solutions I used took a long ass time and a lot of tries before they grew something. I tried almost every trick in the book and it was brutal. To be fair, one of the vials was bunk so that was never going to work. They were both exotic vials and I suspect that they had been in the vendor's fridge since the last ice age.
My latest print to agar germinations all took 48 hours to germinate. I love prints.
|
esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 12 minutes, 6 seconds
|
|
so where can I retrive sone prints if I fail every grow? and wich is the best way to use spore syringes or vial?? I tought agar is a clear and fast way, I tried Lc and wbs too and after 2-3 weeks nothing
--------------------
things does not exist, everything is a process, so we proceed
|
mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
|
Re: Pastywhyte's Easy Agar Tek [Re: esse_jeremy]
#23325331 - 06/09/16 10:15 AM (7 years, 8 months ago) |
|
|
well, you can get spres syringes and prints from vendors. there are shroomery sponsored vendor, if you just search it in google. or, you can trade prints with poeple here who are willing, or get freebies from giveaways. but since you are new, it might be hard to do that. but you never know. go to the AMU threads and there are giveaways for newer users, sometimes.
and yes, agar is a great way to go to make sure you have clean culture before inoculation.
if you arent going to use agar, then take the syringe and do pf tek with it. just look up pf tek, there are tons of research materials and even lots of videos on it. it's really ewasy to make your own brf cakes and inoc those in a SAB with your syringe, while flame sterilizing your needle before each inoc. just do some research and youll find out what you need to know.
when you search for post/threads, filter them by 3 years or newer, and click the TC box, for the least amount of outdated info.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
|
Myko Fanatikos
Rando



Registered: 02/18/16
Posts: 283
Loc: Earth
Last seen: 10 months, 26 days
|
Re: Pastywhyte's Easy Agar Tek [Re: esse_jeremy]
#23325353 - 06/09/16 10:22 AM (7 years, 8 months ago) |
|
|
Only thing I can add to that is don't make posts asking for spores or trades until that 90 day grace period is up for you and the marketplace is open.
If there is a giveaway then by all means, but you will be warned and you may get banned for asking before your 90.
90 days isn't that long and I just breached it lol its no fun waiting but the rules are put in place for the safety of everyone who uses this site.
--------------------

|
mupetmower
Mower of Muppets



Registered: 03/29/16
Posts: 3,036
Loc: Here and There
|
|
fo sho.
thought i would post a successfully fruiting plate i just noticed, that i can get a clone from for a good, fruiting culture. It is Ecuadorian:


I cant thank Pasty enough for this tek. If it werent for this tek, i wouldnt have gotten into agar as easily as i did. it is perfect for beginners, and i see almost everyone, beginner and expert alike, using Pasty Plates.
So, thank you, again, Pasty! you are an invaluable member o this community, and i think most everyone(except the haters) realize that.
-------------------- -The wise man never stops seeking knowledge.
-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.
Edited by mupetmower (06/09/16 10:28 AM)
|
esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 12 minutes, 6 seconds
|
|
yeah but it's difficult to me to evaluate if a vendor is ok or not......also I'm from EU and lots don't ship here :S
--------------------
things does not exist, everything is a process, so we proceed
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: Pastywhyte's Easy Agar Tek [Re: esse_jeremy]
#23325424 - 06/09/16 10:51 AM (7 years, 8 months ago) |
|
|
Quote:
esse_jeremy said: yeah but it's difficult to me to evaluate if a vendor is ok or not......also I'm from EU and lots don't ship here :S
You can use European vendors. I am for the EU as well. You can rest assured that every sponsor on the shroomery is a good one, more or less. They all go through a vetting process to see if they are trustworthy. When I recieved a bad vial from a sponsor, they sent me a new one without any hassle.
IMO, if your spores are not germinating in LC, they might very well be bunk. Spores to LC is a very bad idea for inoculating jars but it is a good way to get stubborn spores to germinate. My 2nd vial also was tough to germinate but the LCs I inoculated were the first to germinate. There is no difference between a syringe and a vial. I would recommend a syringe because taking spore solution from a vial with a syringe is an extra possible vector for contams.
If you are using agar, buy prints instead of spore solutions, it will make your life much easier. Also you should use half the agar the recipe calls for and a little more nutrition than normal. Slightly higher nutrition and a softer agar recipe will help a lot with spore germination. It is the only way I managed to get germination in 48 hours so it works pretty darn good. I use 1g agar and 2.5g malt extract per 100ml for water. You can also try a warm pour. Basically, what you do is make a soft agar batch and PC it. Once it cools and starts to thicken, you pour that over your spore inoculated plate. You have to be careful not to pour very hot agar, it needs to be warm, just before it starts to gel up or you will kill the spores. A warm pour will sandwich your spores between the old agar and the new agar and the warmth will soften the spore walls and that aids in germination.
I've been told that activated charcoal is a good additive for aiding in spore germination so it might be worth a try if you keep having trouble. You can also try forced hydration on your syringes but you would need either a syringe with a luer lock or you have to plug the end of the syringe somehow.
|
esse_jeremy
the deconstructor


Registered: 05/28/16
Posts: 173
Loc: Italy
Last seen: 12 minutes, 6 seconds
|
|
thanks a lot!!! the next time I'll buy a print and follow your recipe!!
but now that i've use vial...what is the avg time for the spore germination on agar?? if with the print is about 3 days with vial or syringes it take up to 7-14 days to start germinate??? (I didn't know that I was going to use agar when I buy the spores.....this is my first order of spore)
--------------------
things does not exist, everything is a process, so we proceed
|
Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
|
Re: Pastywhyte's Easy Agar Tek [Re: esse_jeremy]
#23327622 - 06/09/16 09:33 PM (7 years, 8 months ago) |
|
|
Germination will vary greatly and there is no real way to predict unless you printed the spores yourself. Fresh spores germinate quicker, old ones take longer or possibly never. Type of media is less important for germination but stiffness is. Use around 1.5% agar for a softer easier to germ media.
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#23328466 - 06/10/16 03:52 AM (7 years, 8 months ago) |
|
|
Type of nutes don't but I was told the nutrient content does, doesn't it?
|
MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
|
|
Not so much. I have played with lots of different nutrition levels and sources for spore germination. They always germinate within the same time frame regardless of nutrition.
|
Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
|
Re: Pastywhyte's Easy Agar Tek [Re: MudaFuka]
#23328607 - 06/10/16 08:53 AM (7 years, 8 months ago) |
|
|
Strange, I think it was mad season who told me that. So I guess it was just the soft agar that got me fast germination in the end. I never had spores germ in 2 days before except for oyster. Last batch of spore inoculations I did was with 3 cube varieties and Ps. hooshagenii and I was pretty surprised to see them all germ within hours of each other.
|
|