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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: tombosley8]
#23303331 - 06/03/16 12:30 PM (7 years, 8 months ago) |
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Quote:
tombosley8 said:
Quote:
mupetmower said: also, i did mine without putting paper towels on the tops before the foil, a couple times because i forgot about it, and they turned out the same as the one with paper towel. idk what that means =p just giving an observation. i havent tried to do any of them with unsealed, though.
Same here but I use sfd for my filter...
Quote:
tryptkaloids said:
pasty said the paper towel is to keep the micropore from sticking to the foil. micropore is waterproof so we only need the foil to keep water out of the plate.
Ah ok I thought it was also for condensation or keeping the filter from being wet?
It is. When the filter gets wet it then sticks to the foil.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 9,959
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#23303338 - 06/03/16 12:33 PM (7 years, 8 months ago) |
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They're also handy for writing down variety, transfer number, etc., and you can recycle them plenty. I write in a standard BIC pen in small font, then when I'm done, I Sharpie it out and start again. When that side is done, you can flip it over and repeat. When that side is done, you can flip them inside out. I still have paper towels from the first plates I ever made a year ago. 
Also you can use them to wipe off the condensation if you move them directly from the PC to the SAB since they're still clean. I'm not going to recommend that since condensation isn't a big deal and there's no guarantee they stay sterile from the move, but I've done it probably 20+ times without contams.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 23 days
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#23303343 - 06/03/16 12:35 PM (7 years, 8 months ago) |
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So If I use sfd's for my pp5's do I need the paper towels?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: tombosley8]
#23303349 - 06/03/16 12:37 PM (7 years, 8 months ago) |
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Quote:
tombosley8 said: So If I use sfd's for my pp5's do I need the paper towels?
No.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Pastywhyte's Easy Agar Tek [Re: tombosley8]
#23303351 - 06/03/16 12:38 PM (7 years, 8 months ago) |
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Quote:
tombosley8 said: So If I use sfd's for my pp5's do I need the paper towels?
Nope.
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tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 8 months, 23 days
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thanks to all!
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KinokoKomodo
マッシュルーム



Registered: 05/22/16
Posts: 776
Loc: North Florida
Last seen: 2 years, 6 months
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Re: Pastywhyte's Easy Agar Tek [Re: tombosley8]
#23309083 - 06/05/16 12:46 AM (7 years, 8 months ago) |
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If I'm going to steam sterilize my pasty plates is there anything I need to change about this besides steaming for ~2 hours? Maybe a looser agar mixture or anything like that? Tips and tricks?
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,402
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Don't make a looser agar mixture. Follow the recipe exactly.
You'll get less evaporation because you're using a lower temperature. It will all even out.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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KinokoKomodo
マッシュルーム



Registered: 05/22/16
Posts: 776
Loc: North Florida
Last seen: 2 years, 6 months
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Quote:
elasticaltiger said: Don't make a looser agar mixture. Follow the recipe exactly.
You'll get less evaporation because you're using a lower temperature. It will all even out.
Thank you!
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deagle5
Sir Flumph



Registered: 12/01/15
Posts: 60
Last seen: 2 months, 23 days
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Pasty my darling, is this a workable method sort of combining yours with another one?
Basically using light malt extract and agar powder. Method is as follows 1. Combine LME + Agar powder in a glass with water + swirl until powder has disolved. 2. Add food colouring 3. Pour into PP5 containers and customize the lids in your tek + add paper towels on top with foil wrapped. 4. Place in PC + Add water + lid. 5. Leave weight off + place heat on high until steady stream of steam. 6. PC for 30 mins at 15psi once weight has been added + turned down to med/low heat. 7. Leave overnight in PC to cool and transfer to SAB for use.
Does that method still seem ok? Just wanted another opinion
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Pastywhyte's Easy Agar Tek [Re: deagle5]
#23313462 - 06/06/16 08:24 AM (7 years, 8 months ago) |
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Umm, isn't that basically the tek?
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: deagle5] 1
#23313469 - 06/06/16 08:27 AM (7 years, 8 months ago) |
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Use any recipe you want provided its geared to fungi. Malt, potatos, carrots, cornstarch, turnips, V8, beets, dogfood, cat kibble, grainwater, etc, all make good bases. Add simple sugars at around 1% or omit if the base is high in simple sugar to begin with (like carrot or beet). Simple sugars are things like dextrose, honey, karo, SITR. Its good to change things up regularly to keep the cultures aggressive.
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deagle5
Sir Flumph



Registered: 12/01/15
Posts: 60
Last seen: 2 months, 23 days
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Quote:
Supalemonhaze said: Umm, isn't that basically the tek?
im not putting anything on the stove. wondered if its hampering the potential a little bit or not. or what the actual point is for putting it into a pan and cooking it off
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Pastywhyte's Easy Agar Tek [Re: deagle5]
#23313525 - 06/06/16 08:50 AM (7 years, 8 months ago) |
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To melt it? Depends on the agar mostly. Some agar melts in cold water, normally myco grade is like that. But agar bars or flakes need heat and time before they melt. Some powders also don't melt easily, I avoid chinese sellers for this reason. I bought some agar powder from ebay, gel strength sucked balls, if I tilted the plate the agar would fall down in a blob at the bottom and no matter how agar rich the mix is, it just wouldn't gel properly. It took forever to melt it as well, kinda funny yet sick in the same time, it was advertised as "quick dissolve".
Bought some from the UK and I fell in love with the stuff. Melts in cold tap water so I just throw it my the bottle, add tap water and swirl it around a few times and bam! ready for the PC.
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 5 months
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I love buying my agar off the chinese... all us white guys look the same to them and still everytime I go into his shop buying pokal agar he looks at me funny and says "al YU gonna cook with agal agal??"
hmm that just sounded like racial slur but its pretty funny IRL
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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Re: Pastywhyte's Easy Agar Tek [Re: spacechildo]
#23313629 - 06/06/16 09:34 AM (7 years, 8 months ago) |
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Out of all the things in the world that are racist, finding engrish funny really isn't one of them, engrish is fucking hilarious.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole]
#23313682 - 06/06/16 09:54 AM (7 years, 8 months ago) |
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"da gowrden twiangle"
^^chinese buffet
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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spacechildo
proletarians rise up


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 5 months
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if anything I think the asian dude's the racist one. White boys need aguel aguel too!
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dankington
The Stranger




Registered: 03/14/15
Posts: 4,577
Loc: 8te
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Re: Pastywhyte's Easy Agar Tek [Re: spacechildo]
#23314392 - 06/06/16 01:58 PM (7 years, 8 months ago) |
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YEAH!!!

That'd be more appropriate if I were asian though.
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deagle5
Sir Flumph



Registered: 12/01/15
Posts: 60
Last seen: 2 months, 23 days
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Quote:
Supalemonhaze said: To melt it? Depends on the agar mostly. Some agar melts in cold water, normally myco grade is like that. But agar bars or flakes need heat and time before they melt. Some powders also don't melt easily, I avoid chinese sellers for this reason. I bought some agar powder from ebay, gel strength sucked balls, if I tilted the plate the agar would fall down in a blob at the bottom and no matter how agar rich the mix is, it just wouldn't gel properly. It took forever to melt it as well, kinda funny yet sick in the same time, it was advertised as "quick dissolve".
Bought some from the UK and I fell in love with the stuff. Melts in cold tap water so I just throw it my the bottle, add tap water and swirl it around a few times and bam! ready for the PC.
from the uk? which one pretty please?
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