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Xevtious
Stranger
Registered: 03/30/16
Posts: 97
Last seen: 7 years, 5 months
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Funky mycelium color change and regrowth. To birth or not to birth?
#23305674 - 06/04/16 12:58 AM (7 years, 8 months ago) |
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So I have 2 jars showing this strange slight Browning color change (GT cubes), and i can see new white mycelium colonizing the old mycelium that changed color.
 What's going on here? Is this safe to birth when the new mycelium finish colonizing the old?
-------------------- My name is Xev, and I am a newb. No matter how much research I think I've done, I don't know better than tek writers. I am not a mycologist. I will follow the tek and not deviate. There is no such thing as "good enough". I will do it right or I will not do it at all.
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tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 11 months
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: Xevtious]
#23305692 - 06/04/16 01:06 AM (7 years, 8 months ago) |
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Bacth of batera
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Cameron1996
Stranger
Registered: 11/28/15
Posts: 384
Last seen: 1 year, 10 months
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: tump]
#23305712 - 06/04/16 01:15 AM (7 years, 8 months ago) |
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bacteria... smell it
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George Sears
43rd President of the US



Registered: 02/23/13
Posts: 1,477
Loc: New Mexico
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: tump]
#23305714 - 06/04/16 01:15 AM (7 years, 8 months ago) |
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Super bacteria-ridden. Toss it.
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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 25 days, 8 hours
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: George Sears]
#23305864 - 06/04/16 03:06 AM (7 years, 8 months ago) |
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First off why do you have foil on, and second what's under it, how is your lid set up?
Second did you flame sterilize your syringe and then innovulate *without* touching it / wiping the needle with ISO after flaming it?
--------------------
 Amateur Mycologists United AMU Q&A
Edited by mushpunx (06/04/16 04:27 AM)
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,418
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: mushpunx]
#23305952 - 06/04/16 04:09 AM (7 years, 8 months ago) |
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Nothing is colonizing anything. That is contamed to all fuck. Can't you see the color-change saturating the entire area.
Understand there is no 'win or lose' in this. The bacteria already won.
If you birth that jar it will spend it's entire life spending resources fighting off contamination.
I dare you to take the lid off and smell it.
Contamination cannot be 'destroyed' by healthy mycelium.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: mushpunx]
#23305990 - 06/04/16 04:46 AM (7 years, 8 months ago) |
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Quote:
mushpunx said:
First off why do you have foil on, and second what's under it, how is your lid set up?
Second Thirdly, did you flame sterilize your syringe and then innovulate *without* touching it / wiping the needle with ISO after flaming it?
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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 25 days, 8 hours
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: Supalemonhaze]
#23306172 - 06/04/16 07:12 AM (7 years, 8 months ago) |
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Heh
--------------------
 Amateur Mycologists United AMU Q&A
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Xevtious
Stranger
Registered: 03/30/16
Posts: 97
Last seen: 7 years, 5 months
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: mushpunx]
#23306524 - 06/04/16 09:46 AM (7 years, 8 months ago) |
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Yes, I used impeccable sterile method. This jar and it's brother were doing very well and growing very strongly up until about 95% colonization and then they just.... Died... I think that jar got too warm or something. I figured it was contaminated, I was just intrigued by the regrowth areas.
-------------------- My name is Xev, and I am a newb. No matter how much research I think I've done, I don't know better than tek writers. I am not a mycologist. I will follow the tek and not deviate. There is no such thing as "good enough". I will do it right or I will not do it at all.
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Xevtious
Stranger
Registered: 03/30/16
Posts: 97
Last seen: 7 years, 5 months
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: elasticaltiger]
#23306739 - 06/04/16 11:01 AM (7 years, 8 months ago) |
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Quote:
elasticaltiger said:
Understand there is no 'win or lose' in this. The bacteria already won.
If you birth that jar it will spend it's entire life spending resources fighting off contamination.
I dare you to take the lid off and smell it.
Contamination cannot be 'destroyed' by healthy mycelium.
Challenge accepted. Opened the lids and took a big whiff, no funny smell. I was surprised, mean anything or still garbage?
-------------------- My name is Xev, and I am a newb. No matter how much research I think I've done, I don't know better than tek writers. I am not a mycologist. I will follow the tek and not deviate. There is no such thing as "good enough". I will do it right or I will not do it at all.
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mushpunx
Fungus Punk


Registered: 04/20/14
Posts: 13,394
Last seen: 25 days, 8 hours
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: Xevtious]
#23306763 - 06/04/16 11:12 AM (7 years, 8 months ago) |
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Quote:
Xevtious said: Yes, I used Nnmimpeccable sterile method. This jar and it's brother were doing very well and growing very strongly up until about 95% colonization and then they just.... Died... I think that jar got too warm or something. I figured it was contaminated, I was just intrigued by the regrowth areas.
Well for one the foil comes off right before inoculation and stays off. It impedes gas exchange.
You didn't answer my questions though, did you wipe the needle *after* flaming it?
You say too hot, what w ere the temps in your house?
Still garbage. Bacteria doesnt always smell.
--------------------
 Amateur Mycologists United AMU Q&A
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Xevtious
Stranger
Registered: 03/30/16
Posts: 97
Last seen: 7 years, 5 months
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: mushpunx]
#23307628 - 06/04/16 03:55 PM (7 years, 8 months ago) |
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I had my jars in an incubator until I noticed the color change, then switched to room temp where they all faired much better anyway. I think one spot on the elctric blanket got super warm and killed the bottom of the jars or something, because both of these suspect jars were in the center of the tub and touching each other. The mycelium towards the tops look healthy, and appear to be overtaking the old mycelium very quickly(line was drawn 48 hours prior to picture)

To answer your question, I used an alc lamp to flame the needle and swabbed with a fresh alc swab before each inoculation. It was incredibly tedious for 30 fucking jars and I'm doing agar to bulk next time just to skip this step lol.
-------------------- My name is Xev, and I am a newb. No matter how much research I think I've done, I don't know better than tek writers. I am not a mycologist. I will follow the tek and not deviate. There is no such thing as "good enough". I will do it right or I will not do it at all.
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George Sears
43rd President of the US



Registered: 02/23/13
Posts: 1,477
Loc: New Mexico
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: Xevtious]
#23307655 - 06/04/16 04:08 PM (7 years, 8 months ago) |
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Regardless of what you think, that new mycelium is only fighting a bacterial contamination. Also, you're not supposed to wipe a syringe with alcohol after flame sterilizing.. That heat blanket is also a big red flag. Never use any type of direct, external heating to keep jars warm. The mycelium produces its own heat inside that jar and you adding more than enough to kill it was probably the primary cause of that looking the way it does. Higher temps also encourage bacterial growth, which is really easy to get with BRF since you're not fully sterilizing in a PC or autoclave.
As for having gone through this process for 30 jars.. Well shit, lol. You're definitely not wrong to want to go agar>grain> bulk, and you may want to start rounding up the stuff for all that right now in case all these end up going bad and you have to wait for a whole new batch of jars and shit to colonize.
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Xevtious
Stranger
Registered: 03/30/16
Posts: 97
Last seen: 7 years, 5 months
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: George Sears]
#23307737 - 06/04/16 04:45 PM (7 years, 8 months ago) |
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Already got wishlists set up lol. And I'm not concerned about this batch being fucked, I birthed 22 very clean healthy jars last night that are currently dunking lol. Rolling and fruiting tonight. 6 more just need another week tops, and then the 2 icky ones. I should have some healthy spore prints off this batch, or I might do a live tissue transplant to agar and clone my prettiest mushroom lol
-------------------- My name is Xev, and I am a newb. No matter how much research I think I've done, I don't know better than tek writers. I am not a mycologist. I will follow the tek and not deviate. There is no such thing as "good enough". I will do it right or I will not do it at all.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: Xevtious]
#23308087 - 06/04/16 06:56 PM (7 years, 8 months ago) |
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Yeah, cakes in an incubator will do that if temp goes up too much. You should get rid of the incubator, especially now that it's almost summer.
You can always birth anything that manages to colonize completely in the presence of bacteria but you should manage your expectations of it's perfomance.
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George Sears
43rd President of the US



Registered: 02/23/13
Posts: 1,477
Loc: New Mexico
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: Xevtious]
#23308094 - 06/04/16 06:57 PM (7 years, 8 months ago) |
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Clone yourself a good cluster and you'll have a great monotub by the time you move onto grains and bulk.
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Cameron1996
Stranger
Registered: 11/28/15
Posts: 384
Last seen: 1 year, 10 months
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: elasticaltiger]
#23308543 - 06/04/16 09:20 PM (7 years, 8 months ago) |
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Quote:
elasticaltiger said: Nothing is colonizing anything. That is contamed to all fuck. Can't you see the color-change saturating the entire area.
Understand there is no 'win or lose' in this. The bacteria already won.
If you birth that jar it will spend it's entire life spending resources fighting off contamination.
I dare you to take the lid off and smell it.
Contamination cannot be 'destroyed' by healthy mycelium.
hahahaha that shit stinks so bad itll make a grown man cry, throw up , and black out all at the same time
u say it didnt stink.. break the bottom open outside.. your cultures should be the same color exept metabolites and brusing and when u take them out of the jar it should stink like pure shrooms... some contams do smell like shrooms tho but youll be able to see it and tell its not
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Cameron1996
Stranger
Registered: 11/28/15
Posts: 384
Last seen: 1 year, 10 months
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: Xevtious]
#23308551 - 06/04/16 09:23 PM (7 years, 8 months ago) |
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yeah agar and you might actually salvage some mycelium off that thing
good practice if u do anyways
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Cameron1996
Stranger
Registered: 11/28/15
Posts: 384
Last seen: 1 year, 10 months
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: Supalemonhaze]
#23308561 - 06/04/16 09:24 PM (7 years, 8 months ago) |
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Quote:
Supalemonhaze said: Yeah, cakes in an incubator will do that if temp goes up too much. You should get rid of the incubator, especially now that it's almost summer.
You can always birth anything that manages to colonize completely in the presence of bacteria but you should manage your expectations of it's perfomance.
Room temp works great unless your fucking with woodlovers, then u need it cold
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Xevtious
Stranger
Registered: 03/30/16
Posts: 97
Last seen: 7 years, 5 months
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Re: Funky mycelium color change and regrowth. To birth or not to birth? [Re: Cameron1996]
#23310435 - 06/05/16 12:18 PM (7 years, 8 months ago) |
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I think I'm going to keep the co laminated jars in isolation for observation/educational purposes.
Yea I trashed the incubator, kept in a sealed tub they got up to 76-77 all by themselves lol. Got my 22 jars that were ready dunked and rolled and in the fruiting chamber. Kinda overestimated the amount of space I had though, don't really have space for my other 6 that birth next week xD
-------------------- My name is Xev, and I am a newb. No matter how much research I think I've done, I don't know better than tek writers. I am not a mycologist. I will follow the tek and not deviate. There is no such thing as "good enough". I will do it right or I will not do it at all.
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