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OfflineShroom_walla
Stranger

Registered: 03/16/16
Posts: 59
Loc: Australia
Last seen: 2 years, 6 months
Re: Pastywhyte's Easy Agar Tek [Re: Myko Fanatikos]
    #23286498 - 05/29/16 11:49 PM (7 years, 8 months ago)

Thanks Pasty!

Good simple agar tek, made some a few nights ago, easy to read and easy to follow.

I had to use different style containers but otherwise followed the procedure to the letter, good stuff.


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: Pastywhyte's Easy Agar Tek [Re: Shroom_walla]
    #23292511 - 05/31/16 02:40 PM (7 years, 8 months ago)

I transfered and still having problems.


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InvisibleMunchauzen
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Registered: 06/22/11
Posts: 14,343
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Re: Pastywhyte's Easy Agar Tek [Re: Thoky.ykoht]
    #23292543 - 05/31/16 02:47 PM (7 years, 8 months ago)

Quote:

Thoky.ykoht said:
I transfered and still having problems.




I see some clean growth that has separated from the bacteria. get in there and transfer that ASAP, otherwise you might have to do an agar sandwhich to save that


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
    #23292551 - 05/31/16 02:50 PM (7 years, 8 months ago)

Which photo are you referring to?
What's an agar sandwich?


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InvisibleMudaFuka
Poppin bottles
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Registered: 12/14/13
Posts: 18,648
Loc: Canada Flag
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Re: Pastywhyte's Easy Agar Tek [Re: Thoky.ykoht]
    #23292562 - 05/31/16 02:53 PM (7 years, 8 months ago)

I'd say around 8 o'clock on the last photo. Take the smallest piece of mycelium posible. Like a quarter the size if a grain of rice.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     


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InvisibleMunchauzen
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Registered: 06/22/11
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Re: Pastywhyte's Easy Agar Tek [Re: Thoky.ykoht]
    #23292566 - 05/31/16 02:54 PM (7 years, 8 months ago)

Quote:

Thoky.ykoht said:
Which photo are you referring to?
What's an agar sandwich?



4th picture. 4 oclock - 11 oclock.

agar sandwhich is to just take a 2nd agar plate, and drop the agar puck on top of the first one. the bacteria can't grow up through it, but cube myc can.


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Offlinedeagle5
Sir Flumph
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Registered: 12/01/15
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Last seen: 2 months, 23 days
Re: Pastywhyte's Easy Agar Tek [Re: Bloby]
    #23292568 - 05/31/16 02:55 PM (7 years, 8 months ago)

Quote:

Bloby said:
I've just bought some of these (link) pp5 containers so I can have a go at this tek. I have just one question, in the description of the containers it says "Always leave the lid 1/4 open when sterilising." I haven't seen this mentioned anywhere else so I think ill try just four plates 2 open 2 closed and see what happens the first time around.

Does anyone else have any info on this?




Is that company reputable?


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: Pastywhyte's Easy Agar Tek [Re: deagle5]
    #23292631 - 05/31/16 03:12 PM (7 years, 8 months ago)

How long does it usually take the myc to grow through?


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: Pastywhyte's Easy Agar Tek [Re: Thoky.ykoht]
    #23292988 - 05/31/16 04:52 PM (7 years, 8 months ago)

Do you think it'd be easier to transfer away from a different starter plate?


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OfflineMyko Fanatikos
Rando
I'm a teapot


Registered: 02/18/16
Posts: 283
Loc: Earth
Last seen: 10 months, 26 days
Re: Pastywhyte's Easy Agar Tek [Re: Thoky.ykoht]
    #23293015 - 05/31/16 04:59 PM (7 years, 8 months ago)

Are you using a sab?


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: Pastywhyte's Easy Agar Tek [Re: Myko Fanatikos]
    #23293080 - 05/31/16 05:16 PM (7 years, 8 months ago)

Yes


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InvisibleThoky.ykoht

Registered: 10/08/13
Posts: 399
Re: Pastywhyte's Easy Agar Tek [Re: Thoky.ykoht]
    #23293098 - 05/31/16 05:22 PM (7 years, 8 months ago)

I have 8 plates I used a swab on. They haven't shown any contam or growth.

The myc above is from a jar a spawned, it was leftover in the bottom and had begun to pin so I tried to transfer it to agar to save it. If I can't I'm back to square one.
I have some syringes but I never have any luck on agar with spore solution, my plates for whatever reason always have pooling water. Even the ones above sat a week before transferring anything to them to try to fight the condensation/pooling water.
These are all on Lightmalt/water/agar


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OfflineMyko Fanatikos
Rando
I'm a teapot


Registered: 02/18/16
Posts: 283
Loc: Earth
Last seen: 10 months, 26 days
Re: Pastywhyte's Easy Agar Tek [Re: Thoky.ykoht]
    #23293277 - 05/31/16 06:09 PM (7 years, 8 months ago)

I use potatoes flakes/agar/water. I also started with live myc from a pf jar because my initial try with spores never took off

My biggest issue from my first plates was pooling water also. I started bringing my pressure cooker up a lot slower...a loot slower, and it seemed to help quite a bit with condensation.

I've had had some issues with bacteria on my agar as well. I usually just open it up inside my sab and cut the bacteria out of the agar. Probably not the best advice but it's worked for me.

The way I see it, you have myc growing on agar. As long as your plates are sterile and you don't leave the lid open for a million years to transfer then your far from square one.

If there is even a small sliver of myc you can transfer away from that contaminate then you should be good to go.

It may take a few more transfers but if your persistent you'll see mushies in your future.


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Edited by Myko Fanatikos (05/31/16 06:12 PM)


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InvisibleBloby
Stranger


Registered: 06/04/10
Posts: 17
Re: Pastywhyte's Easy Agar Tek [Re: deagle5]
    #23295418 - 06/01/16 09:11 AM (7 years, 8 months ago)

Quote:

deagle5 said:
Is that company reputable?




That was my first time using them but my containers arrived in a couple of days, well packaged etc. After doing my test run of 6 plates I brought the pressure up super slowly and allowed to cool before opening the cooker and non on them have warped (even the plate I left closed). My cooker only goes to 11.6 PSI so this might have had an influence.

I'll report back once I've used them a few more times, also its been 4 days since having a play in the SAB and non have contaminated yet so fingers crossed the seal holds.


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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
Posts: 9,959
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Re: Pastywhyte's Easy Agar Tek [Re: Bloby]
    #23298420 - 06/02/16 12:55 AM (7 years, 8 months ago)

A bunch of my PP's got fucked with bacteria when my fridge's cycle went through the increasing summer heat. I probably should've be storing plates that long anyway.

Anyway, I don't have lunchboxes lying around for insulation. What do you guys think about stacking them in glass mason jars?


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 4 days, 6 hours
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Re: Pastywhyte's Easy Agar Tek [Re: stareatclouds]
    #23301461 - 06/02/16 09:16 PM (7 years, 8 months ago)

why? just transfer away...


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
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Invisiblestareatclouds
star eat clouds?
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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #23301540 - 06/02/16 09:35 PM (7 years, 8 months ago)

What? I'm talking about protecting the myc from fridge cycles, not making transfers.


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Offlinetryptkaloids
Learner
I'm a teapot


Registered: 02/08/15
Posts: 12,649
Loc: Exact Center
Last seen: 4 days, 6 hours
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Re: Pastywhyte's Easy Agar Tek [Re: stareatclouds]
    #23301604 - 06/02/16 09:57 PM (7 years, 8 months ago)

I don't understand what's happening... temperatures shouldn't add bacteria just make it easier to grow... leaving it in the fridge shouldn't change a thing


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 


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Invisiblestareatclouds
star eat clouds?
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Registered: 09/29/14
Posts: 9,959
Trusted Cultivator
Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #23301673 - 06/02/16 10:19 PM (7 years, 8 months ago)

The temperature changes cause water to start pooling which can't be good for anything. In any case, I need to shield them from the temperature changes which is what I'm asking about. Ziploc bags don't work.


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Invisiblewowimflabbergasted
supercalifragilistic
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Registered: 07/16/12
Posts: 18,918
Re: Pastywhyte's Easy Agar Tek [Re: stareatclouds]
    #23301994 - 06/03/16 12:22 AM (7 years, 8 months ago)

Just go to the store and get a $5 lunchbox :shrug: You of all people stare should know not to cut corners.


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