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Amazon Shop for: ½ Pint Jars, HEPA Filter, Paul Stamets, Potato Dextrose, Rye Grain, Wild Bird Seed

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Invisibleeasyskunkin

Registered: 12/23/03
Posts: 565
mycelium died after shaking ?
    #2329740 - 02/13/04 09:35 AM (13 years, 16 days ago)

Hello and thank you for all the answers to my question about the explosions in my PC, I manage a little better now. now I have another problem : I inoculated 5 jars of rye with mycelium grown on PDA substrate, and after 5 days they looked really good and colonized quickly (around 29 celsius)so I shook them as recommended in The Mushroom Cultivator, but now they seem dead and the mycelium doesn't grow anymore. these jars were sealed (I live in a small island and I don't have access to such thing as synthetic filter discs), is it the problem ? I don't think it's contamination from outside because I work in a glove box with HEPA filter and follow sterile procedures. Endospores maybe ? if it's the case will "tyndallisation" help ?


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L'intérêt à croire une chose n'est pas une preuve de l'existence de cette chose.
Voltaire ; Remarques sur les pensées de Pascal (1728)


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InvisiblePrisoner#1M
Even Dumber ThanAdvertized!
 User Gallery

Registered: 01/22/03
Posts: 193,402
Loc: Pvt. Pubfag NutSuck
Trusted Cultivator
Re: mycelium died after shaking ? [Re: easyskunkin]
    #2330025 - 02/13/04 11:40 AM (13 years, 16 days ago)

if you are using grains thee is a greater risk of contaminatio with out autoclaving/pressure cooking... do the kernels have a greasy apearance or is there water that had collected in the bottom of the jars... these are signs of bacterial contaminants... it sometimes sakes a few days for mycelium to recover from shaking and also if the baccilus is present then they may not recolonize at all... best to wait a week or two and see how things progress


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InvisibleSemilanceata
No god, no boss

Registered: 05/26/03
Posts: 841
Loc: República Federal Íbera
Re: mycelium died after shaking ? [Re: easyskunkin]
    #2330061 - 02/13/04 11:49 AM (13 years, 16 days ago)

Can?t you get some polyfill in the island you live ? Polyfill is sold as pillows filling.


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Sr_Setahongo


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Offlinecrazychemist
Spreader ofSpores

Registered: 09/24/03
Posts: 495
Last seen: 11 years, 6 months
Re: mycelium died after shaking ? [Re: easyskunkin]
    #2330360 - 02/13/04 12:55 PM (13 years, 16 days ago)

Use polyfill.  That is what I used even though I probably could have gotten some Tyvek.  My quarts of WBS are moving along nicely.  :smile:  Good luck.


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The worst mistake that you can make is to think you're alive when really you're asleep in life's waiting room.
-Waking Life


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Invisibleeasyskunkin

Registered: 12/23/03
Posts: 565
Re: mycelium died after shaking ? [Re: crazychemist]
    #2330612 - 02/13/04 01:46 PM (13 years, 16 days ago)

thank you all I will try to find polyfill. The thing is with my jars, they don't show any trace of contamination at all, they just don't grow anymore after shaking. I think now it is the total lack of air exchange. Thank you for helping ! Pedro


--------------------
L'intérêt à croire une chose n'est pas une preuve de l'existence de cette chose.
Voltaire ; Remarques sur les pensées de Pascal (1728)


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InvisibleBi0TeK
elephant man

Registered: 11/07/02
Posts: 3,002
Loc: Yorkshire Moors, Great Br...
Re: mycelium died after shaking ? [Re: easyskunkin]
    #2330645 - 02/13/04 01:55 PM (13 years, 16 days ago)

If you find polyfill hard to source try micropore tape, works great and they sell it at most pharmacies.


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PROMOTE BACTERIA. THEY'RE THE ONLY CULTURE SOME PEOPLE HAVE.


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Offlineamyloid
Stranger
Registered: 03/08/03
Posts: 980
Last seen: 3 years, 1 month
Re: mycelium died after shaking ? [Re: easyskunkin]
    #2330689 - 02/13/04 02:07 PM (13 years, 16 days ago)

if you live in the united states, or a location the USPS services you can have them send you free tyvek envelopes, which work as great and free filter disks! i ordered enough for the rest of my life(20 packs of 10), they arrived 1 week later.


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"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein


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Amazon Shop for: ½ Pint Jars, HEPA Filter, Paul Stamets, Potato Dextrose, Rye Grain, Wild Bird Seed

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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