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Amazon Shop: ½ Pint Jars, Vermiculite

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OfflineBaby_Hitler
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Popcorn contams, me blames tyvec
    #2328715 - 02/13/04 12:29 AM (14 years, 15 days ago)

I made up a dozen jars of popcorn about 2 weeks ago with pieces of tyvec for lids to see if they would keep, and guess what. out of twelve, 5 of them have clear signs of contamination. They were PC'd for an hour at 15 lbs and like the title says I blame the tyvec.

They all contaminated on the top first is the main reason I blame the tyvec, or at least the seals. Something got in after they were completely sterilized.


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[quote]RogerRabbit said:
Actually, it's very easy to isolate a super producing sclerotia strain.

Follow the strain isolation technique on Let's Grow Mushrooms, and then select sectors early which are brown in color.  By the second or third transfer, you'll see stones developing, and this is only about 1 month after the original swipe of spores on agar.

Now, take each stone and move it to a new dish.  Soon, the mycelium will grow out and you'll see fresh stones developing, and if they're good strains, the sclerotia is forming long before the mycelium reaches the edge of the plate.  Pick strains which form four or five stones within two weeks and use these for your grain masters.
RR [/quote]


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Invisiblekeyeghost
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Registered: 09/04/02
Posts: 123
Re: Popcorn contams, me blames tyvec [Re: Baby_Hitler]
    #2328861 - 02/13/04 01:09 AM (14 years, 15 days ago)

My friend has had similar results w/ popcorn and tyvec. What did your contams look like? My friends looked like the popcorn was growing grey hairs.

Ki


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"time couldn't end me, even the great devil Satan who tried to befriend me, understand the equality, God in the bodily form, lettin my knowledge be born" RZA as Prince Rakeem


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InvisibleZapotecorum
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Registered: 01/15/04
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Re: Popcorn contams, me blames tyvec [Re: Baby_Hitler]
    #2328883 - 02/13/04 01:14 AM (14 years, 15 days ago)

tyvec compaired to the layer of verm in a pf style cake is worthless. every time i have used tyvec in bulk grain inoculations i have had major loses compaired to almost no loses with pf style jars employing the 1/4 - 1/2 layer of verm.


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Invisiblekeyeghost
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Registered: 09/04/02
Posts: 123
Re: Popcorn contams, me blames tyvec [Re: Zapotecorum]
    #2328983 - 02/13/04 01:37 AM (14 years, 15 days ago)

Yeah that is the difference in PF style cakes and bulk grains I would guess. My friend finds grains much more fufilling than anything pf style.

Ki


--------------------
"time couldn't end me, even the great devil Satan who tried to befriend me, understand the equality, God in the bodily form, lettin my knowledge be born" RZA as Prince Rakeem


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Amazon Shop: ½ Pint Jars, Vermiculite

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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