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InvisibleHippie3
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Registered: 11/06/99
Posts: 3,090
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Re: The Coffee Project [Re: ragadinks]
    #2321775 - 02/10/04 07:24 PM (20 years, 1 month ago)

anyone want to see rodger's latest project ?
Quote:

It was done in honor of the original artist who drew the image on a cave wall in Algeria 7,000 years ago.






see The Cave Bee


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Invisiblejoe_millionaire
Lifelong Student

Registered: 11/15/03
Posts: 220
Re: The Coffee Project [Re: Hippie3]
    #2321951 - 02/10/04 08:12 PM (20 years, 1 month ago)

my girl innoculated 6 mycobags of 1000 grams rye grass seed and 500 ml coffee (folgers - 1 tablespoon per cup), and 500 ml H20. She innoculated w/ Mex A for sclerotia production. Of course she will keep everyone posted as she has 6 other bags that were just soaked w/ water (control group).

peace all


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"Ekam Sat Vipras Bahudda Vidante"
The One Truth is named by the wise in many ways.

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OfflineEkstaza
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Re: The Coffee Project [Re: Hippie3]
    #2323513 - 02/11/04 08:59 AM (20 years, 1 month ago)

I was just brewing some coffee to give this thing a try with some millet I have and I got to thinking about a variable that some might have overlooked. I personally like to make my coffee strong as shit and others I know make theirs where it looks like tea. This variance would certainly effect the final outcome of any project and deffinately would skew the results of a large community experiment. Perhaps some kind of standard should be established.


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YOUR EXPERIENCE WITH ANY GIVEN DRUG ISN'T THE DEFINITIVE MEASURE OF THE DRUGS EFFECTS.

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InvisibleHippie3
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Re: The Coffee Project [Re: Ekstaza]
    #2323564 - 02/11/04 09:32 AM (20 years, 1 month ago)

the current standard is 50% of 'normal' brewed coffee,
according to the measurements on the pkg.
according to my can of foldger's,
that works out to 1/2 tablespoon per cup .


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Invisiblejoe_millionaire
Lifelong Student

Registered: 11/15/03
Posts: 220
Re: The Coffee Project [Re: jarhead]
    #2323661 - 02/11/04 10:09 AM (20 years, 1 month ago)

Quote:

jarhead said:
Primary Nutrients :


Nitrogen 1.45%
Phosphorus ND ug/g
Potassium 1204 ug/g

Secondary Nutrients :

Calcium 389 ug/g
Magnesium 448 ug/g
Sulfur high ug/g


Notes:

ND = indicates sample is below detection limit
ug/g = microgram / gram "





So the question becomes, is the mycelium growing at a faster rate (if it is at all) because of the caffeine or because of the extra nutrients that coffe provides.

So once (and if) it is established that coffee (at 1/2 tablespoon per cup) speeds colonization, then the next step would be to use caffeine in a powdered form to one test group and coffee as the control.

Just a thought. But I am getting ahead of myself...


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"Ekam Sat Vipras Bahudda Vidante"
The One Truth is named by the wise in many ways.

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InvisibleYesItsMe
Homeless GOHOME !...
Registered: 10/29/03
Posts: 253
Loc: Working for Piss ;...
Re: The Coffee Project [Re: Hippie3]
    #2325962 - 02/12/04 06:33 AM (20 years, 1 month ago)

I dont know if its new for you , but i find this link :
A PLAN TO REGENERATE THE BY-PRODUCTS OF COFFEE BEAN CULTIVATION

--> We have chosen two species of saprophytes: the Oyster (Pleurotus Oystretus) and the King Stropharia (Rugosa Annulata ). Oyster mushrooms are recognized as capable of bioremediation of coffee waste. We have grown Oyster mushrooms successfully on coffee grounds, and experience leads us to believe that King Stropharia will grow on an extremely wide range of media . <--

Other link :

Effect of the nitrogen source on caffeine degradation by Aspergillus tamarii

--> Caffeine degradation as well as biomass production were characterized. <--


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God save the Queen

Edited by YesItsMe (02/12/04 06:50 AM)

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InvisibleHippie3
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Re: The Coffee Project [Re: YesItsMe]
    #2326073 - 02/12/04 08:40 AM (20 years, 1 month ago)

you know,
trying some dilute coffee
or caffeine
in some agar plates
might make a great experiment.
thx for those links.


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Offlinechillywilly
Eat well - Diethome
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Registered: 03/31/03
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Re: The Coffee Project [Re: Hippie3]
    #2326553 - 02/12/04 11:32 AM (20 years, 1 month ago)

Has anybody considered trying to grow on straight coffee beans yet?
I wonder if there would be a difference between roasted and green beans?

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OfflineRohypnol
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Registered: 08/04/03
Posts: 267
Last seen: 8 years, 7 months
Re: The Coffee Project [Re: Hippie3]
    #2328270 - 02/12/04 07:52 PM (20 years, 1 month ago)

Quote:

Hippie3 said:
you know,
trying some dilute coffee
or caffeine
in some agar plates
might make a great experiment.
thx for those links.




12 agar plates prepared.

6 PDA/ 6 PDA+Coffee

Orissa India
Puero Rico
Malabar

Will report results next saturday - going on vacation.

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InvisibleHippie3
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Re: The Coffee Project [Re: Rohypnol]
    #2329669 - 02/13/04 06:58 AM (20 years, 1 month ago)

what concentration of coffee did you use ?


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OfflineRohypnol
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Registered: 08/04/03
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Re: The Coffee Project [Re: Hippie3]
    #2329808 - 02/13/04 08:34 AM (20 years, 1 month ago)

about 50%

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InvisibleLoki
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Registered: 02/10/04
Posts: 18,296
Loc: Zone ate
Re: The Coffee Project [Re: Rohypnol]
    #2330032 - 02/13/04 09:42 AM (20 years, 1 month ago)

50% ? as in 50% of the liquid used, ie say making up
500ml, you used 250ml of prediluted coffee?

50% seems high.

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OfflineVertigo
Mycovoire
Registered: 03/05/03
Posts: 289
Loc: Panhandle
Last seen: 5 years, 7 months
Re: The Coffee Project [Re: chillywilly]
    #2330077 - 02/13/04 09:52 AM (20 years, 1 month ago)

That's interesting because about a year ago I read about a tek where someone innoculated halved beans and that they grew w/o contaming ever. It was a long time ago but I think I may have found it through erowid. I'll see about finding it again.


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"One side will make you grow taller, and the other side will make you grow shorter."

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Invisiblehevvy_psi
groover

Registered: 01/29/04
Posts: 10,446
Re: The Coffee Project [Re: Hippie3]
    #2330334 - 02/13/04 10:47 AM (20 years, 1 month ago)

what about making PF jars with vermiculite and ground coffee?


--------------------
egocentrism is bangin on the door
self-destructive selflessness
seeps out from the core
alone - eyes closed - an empty room
i'm curled on the floor
choose nothing, thus deciding
all the nothing i've in store.

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OfflineExtravagantDream
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Re: The Coffee Project [Re: hevvy_psi]
    #2330457 - 02/13/04 11:16 AM (20 years, 1 month ago)

a note on the coffee brew soak.

I like to rinse mine well afterwards, so the question is, does the coffee actually absorb through the grain shell or does it sort of just stick to the sides?

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InvisibleHippie3
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Re: The Coffee Project [Re: hevvy_psi]
    #2330497 - 02/13/04 11:23 AM (20 years, 1 month ago)

if it were me,
i'd just use 50% dilute coffee
instead of the 1/4 cup of water
normally used.


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Invisiblehevvy_psi
groover

Registered: 01/29/04
Posts: 10,446
Re: The Coffee Project [Re: Hippie3]
    #2330501 - 02/13/04 11:25 AM (20 years, 1 month ago)

and still us the BRF?


--------------------
egocentrism is bangin on the door
self-destructive selflessness
seeps out from the core
alone - eyes closed - an empty room
i'm curled on the floor
choose nothing, thus deciding
all the nothing i've in store.

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InvisibleHippie3
mycotopiate
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Registered: 11/06/99
Posts: 3,090
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Re: The Coffee Project [Re: hevvy_psi]
    #2330630 - 02/13/04 11:51 AM (20 years, 1 month ago)

yes,
and the vermiculite too.


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Invisiblehevvy_psi
groover

Registered: 01/29/04
Posts: 10,446
Re: The Coffee Project [Re: Hippie3]
    #2330650 - 02/13/04 11:56 AM (20 years, 1 month ago)

of course :smile:

thanks :thumbup:


--------------------
egocentrism is bangin on the door
self-destructive selflessness
seeps out from the core
alone - eyes closed - an empty room
i'm curled on the floor
choose nothing, thus deciding
all the nothing i've in store.

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Offlineamyloid
Stranger
Registered: 03/08/03
Posts: 980
Last seen: 10 years, 2 months
Re: The Coffee Project [Re: Hippie3]
    #2330714 - 02/13/04 12:13 PM (20 years, 1 month ago)

i didnt see anyone mentioning trying azures... im very suprised no one has tried fruiting coffee azures indoors. if i had the supplies i would!


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"A human being is part of a whole, called by us the Universe, a part limited in time and space. He experiences himself, his thoughts and feelings, as something separated from the rest--a kind of optical delusion of his consciousness. This delusion is a kind of prison for us, restricting us to our personal desires and to affection for a few persons nearest us. Our task must be to free ourselves from this prison by widening our circles of compassion to embrace all living creatures and the whole of nature in its beauty."
-Al Einstein

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