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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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Offlinecolvet
tonal tripper
Registered: 02/12/04
Posts: 1
Loc: Pacific NW
Last seen: 14 years, 2 months
What did I do wrong?
    #2326480 - 02/12/04 01:15 PM (14 years, 2 months ago)

Greetings

About 7 days ago I innoculated my substrate jars with some Golden Teacher spores, and I'm not seeing any apparent growth right now. I see what appears to be slimy growth (light brown in color) but no bright white flecks or growth. Does it take some jars longer to start growing mycelium than others?

Here's some important info:

Jars: 1/2 Pint Kerr shoulderless
Substrate: Med vermiculite, brown rice flour (organic), distilled water
Sterilization: over an hour on low boil
Conditions: In laundry room with a lamp overhead 1/2 the time. Temp between 70 and 80 F.

Thanks for any help


Edited by colvet (02/12/04 01:21 PM)


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InvisibleLoki
fluid druid
 User Gallery

Registered: 02/10/04
Posts: 15,474
Loc: Zone ate
Re: What did I do wrong? [Re: colvet]
    #2326496 - 02/12/04 01:18 PM (14 years, 2 months ago)

Brown slime could be bacterial contamination.


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OfflineGr0wer
always improving
Male

Registered: 09/16/03
Posts: 6,056
Loc: El Paso, TX Flag
Last seen: 1 month, 8 days
Trusted Cultivator
Re: What did I do wrong? [Re: Loki]
    #2326529 - 02/12/04 01:26 PM (14 years, 2 months ago)

The laundry room prob has lots of airborn contams. Build a bucket in a bucket incubator.


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Offlinegustavo
Stranger
Registered: 01/13/04
Posts: 8
Last seen: 14 years, 1 month
Re: What did I do wrong? [Re: colvet]
    #2326534 - 02/12/04 01:27 PM (14 years, 2 months ago)

when i innoculated w/ golden teacher spores they took a little longer than other strains (amazonian and ecquador)....over a week before any growth. but you shouldn't have light on them at all.


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InvisibleZapotecorum
AlcoHoliC

Registered: 01/15/04
Posts: 186
Loc: my conversion van
Re: What did I do wrong? [Re: colvet]
    #2326707 - 02/12/04 02:17 PM (14 years, 2 months ago)

slime is bad no matter what color. throw away the ones with slime.
its rare what it could be the syringe you used was a dude, it happens. it really makes you second guess your technique. but if not a single jar grows that might be it.


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OfflineGregHimself
Just a guyobssesed withshroomies.
Male User Gallery

Registered: 07/19/03
Posts: 936
Loc: British Columbia, Canada
Last seen: 4 months, 10 hours
Re: What did I do wrong? [Re: Zapotecorum]
    #2326761 - 02/12/04 02:30 PM (14 years, 2 months ago)

no, bacterial contams are hard to deal with, you shouldnt be doing thisi in you laundry room first off, and 2ndly if it is a bacterial contam...which brown slimey usually is, you will get no spore germination...so your screwed i am sorry to say, if you need help with some simple but effective sterile methods, giv e me a pm.


--------------------
"The key to successful cultivation is to match the skills of the cultivator with the right strain on the proper substrate under ideal environmental conditions."
Paul Stamets
Growing Gourmet and Medicinal Mushrooms(Third Edition)


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Offlinerichismalls
Richi-smalls

Registered: 10/19/02
Posts: 768
Loc: the ill state
Last seen: 6 months, 5 hours
Re: What did I do wrong? [Re: colvet]
    #2326878 - 02/12/04 02:55 PM (14 years, 2 months ago)

do you have a pic?


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OfflineTheGanjaGorilla
Shiitake head

Registered: 11/21/02
Posts: 185
Loc: N.E. Indy
Last seen: 3 years, 4 months
Re: What did I do wrong? [Re: colvet]
    #2327237 - 02/12/04 04:21 PM (14 years, 2 months ago)

It sounds like you maybe boned....If you don't have a pressure cooker, you implement the fractional sterilization tek.

Also if you can, injext your jars in the cleanest room possible with little or no air movement. I start my jars in the mudroom of my house. This time of year the rooms temp is around 32 degrees F. I've never had trouble with injection infections before.


--------------------
"Whateva I do what I want..." Eric Catman




"Whateva, I do what I want..." Eric Cartman


TheGanjaGorilla.


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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Pressure Cooker, Vermiculite

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