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InvisibleMatai


Registered: 05/04/14
Posts: 1,016
Loc: NZ Flag
Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Bagels]
    #23257176 - 05/22/16 06:18 AM (7 years, 10 months ago)

Quote:

Bagels said:
 





Those are the most adorable psilocybe I've ever seen  :love:


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InvisibleBagels
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Registered: 07/11/14
Posts: 1,029
Loc: NZ
Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Matai]
    #23270608 - 05/25/16 04:57 PM (7 years, 9 months ago)



The one on the left has a twisted stipe and quite a few are like that. Does anyone know the correct term for that?

I have only found two gnat larva on the mushrooms. I think the flies must not be attracted to them because they are so small.

I quite like them too Matai :smile:

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OfflineDunedinite
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Registered: 04/06/16
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Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Bagels]
    #23271266 - 05/25/16 07:36 PM (7 years, 9 months ago)

I'm wondering if I found one of these myself... Found a mature psilocybe that was maybe 8mm across the cap with a stem about 25mm long and 2mm diameter. Took a spore print of it, might try cultivating it from those spores. Didn't actually take any photos though, didn't think about it until it was quite dried. Then I ate it. It was delicious.


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The Official Kiwi Cultivators Thread - for those of us looking for New Zealand-specific assistance!

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InvisibleHashed
SHROOM HEAD

Registered: 03/04/16
Posts: 1,933
Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Bagels]
    #23271431 - 05/25/16 08:16 PM (7 years, 9 months ago)

Quote:

Bagels said:


The one on the left has a twisted stipe and quite a few are like that. Does anyone know the correct term for that?





Mutation.

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OfflineAlan RockefellerM
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Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Hashed]
    #23271879 - 05/25/16 10:02 PM (7 years, 9 months ago)

Quote:

Hashed said:
Mutation.





That's unlikely - mutation means changed DNA.  This is just regular variation.

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OfflineDunedinite
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Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Alan Rockefeller]
    #23272039 - 05/25/16 10:48 PM (7 years, 9 months ago)

Quote:

Alan Rockefeller said:
Quote:

Hashed said:
Mutation.





That's unlikely - mutation means changed DNA.  This is just regular variation.




I assume there are allelic differences between members of a species? For example the subs with nipples and the subs without.


--------------------
The Official Kiwi Cultivators Thread - for those of us looking for New Zealand-specific assistance!

Check my journal for my attempts at growing mushrooms!

"Don't accept that what's happening
Is just a case of others' suffering
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OfflineHudson
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Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Dunedinite]
    #23272123 - 05/25/16 11:19 PM (7 years, 9 months ago)

Quote:

Dunedinite said:
Quote:

Alan Rockefeller said:
Quote:

Hashed said:
Mutation.





That's unlikely - mutation means changed DNA.  This is just regular variation.




I assume there are allelic differences between members of a species? For example the subs with nipples and the subs without.



Could be, but that may be the result of environmental conditions like substrate, humidity, precipitation and temperature would all influence the fruiting body's growth. I have found what I believe to be a phenotypic variation of P. subaeruginosa, in a small patch of native forest about a hectare in size seem to grow directly from pine needles each year and have distinctly hollow stipes and in my experience have a lower psilocin content than their woodchipped dwelling counterparts.

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OfflineAlan RockefellerM
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Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Hudson]
    #23272197 - 05/25/16 11:52 PM (7 years, 9 months ago)

Quote:

Hudson said:
Could be, but that may be the result of environmental conditions like substrate, humidity, precipitation and temperature would all influence the fruiting body's growth. I have found what I believe to be a phenotypic variation of P. subaeruginosa, in a small patch of native forest about a hectare in size seem to grow directly from pine needles each year and have distinctly hollow stipes and in my experience have a lower psilocin content than their woodchipped dwelling counterparts.





That is cool, check the ITS DNA sequences.  All of the P. subaeruginosa that I have sequenced matches well with this one:  http://www.ncbi.nlm.nih.gov/nucleotide/432176743

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InvisibleBagels
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Registered: 07/11/14
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Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Alan Rockefeller]
    #23272500 - 05/26/16 02:15 AM (7 years, 9 months ago)

Thanks everyone. I think I can scrub the "found growing from pine wood chip" in my original post. There seems to be a variety of woods and very little bark.

I managed a few photos today between the showers. These mushrooms were found even further away from my original finds.

There are mostly native plants and a few exotics. These mushrooms were growing near young akeake green (Dodonaea viscosa)  and clover.





And right next door subs!


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InvisibleHashed
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Registered: 03/04/16
Posts: 1,933
Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Alan Rockefeller]
    #23274761 - 05/26/16 06:24 PM (7 years, 9 months ago)

Quote:

Alan Rockefeller said:
Quote:

Hashed said:
Mutation.





That's unlikely - mutation means changed DNA.  This is just regular variation.




Interesting, I always thought that crazy corkscrew stipes were a mutation not just a regular phenotype.

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InvisibleSigneg
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Registered: 06/09/12
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Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Hashed]
    #23344711 - 06/14/16 07:18 PM (7 years, 9 months ago)

Nice work Bagels, look's like a cool mushroom whatever it is. look forward to the results.:popcorn:

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Offlineantichode
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Registered: 06/19/09
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Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Hashed]
    #23345884 - 06/15/16 03:06 AM (7 years, 9 months ago)

The question on everyone's lips however. When this species has a name and a place in science (which is a thing you can mention to be smart and shit). Will it be added to the biggest and best go to resource for all the information and teks ?

Cool finds. Hella nugz

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InvisibleMike O. Phile
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Registered: 03/07/16
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Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: antichode]
    #23347887 - 06/15/16 03:59 PM (7 years, 9 months ago)

awesome thread Bagels, I'm watching with interest :thumbup:

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InvisibleBagels
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Registered: 07/11/14
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Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Mike O. Phile]
    #23349436 - 06/16/16 12:43 AM (7 years, 9 months ago)

   



Thanks everyone. If you knew had bad my eyesight was you would be surprised I found anything at all lol.

Edited by Bagels (06/16/16 12:50 AM)

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InvisibleBagels
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Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Bagels]
    #23351194 - 06/16/16 01:57 PM (7 years, 9 months ago)

A few more pics showing how small some of them are. The NZ dollar coin is 2.2 cm in diameter.


 




Some of these were quite chocolate brown in colour perhaps because of the frost.

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OfflineAlan RockefellerM
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Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Bagels]
    #23381006 - 06/25/16 02:28 PM (7 years, 8 months ago)

ITS sequence: 

atgaACTTGGCTCGGTTGCAGCTGGTCCTCTAGAGGGCATGTGCTCGCCGTGTCATCTTTATCTCTCCACCTGTGCACCTTTTGTAGACCTGGATTAGTTAATTTTCCGAGGAAACTCGGTCGGGAGGATTGCTTTCACAAGCTCTCCTTGCAATTAACCCAGGCCTACGTTTTCATATACCCCAAAGAATGTAACAGAATGTATTATATTGGCCTTGTGCCTATAAACTATATACAACTTTCAGCAACGGATCTCTTGGCTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACCTTGCGCTCCTTGGTATTCCGAGGAGCATGCCTGTTTGAGTGTCATTAAATTCTCAACCTTACCAGCTTTTGCTGATAATGGCTTGGATGTGGGGGTCTTTTTGCTGGCTTCGTTAAGAGGTCTGCTCCCCTTAAATGTATTAGCCGGTGCCCCGCGCAGAGCCGTCTATTGGTGTGATAATTATCTACGCCGTGGACCTTTGCATGAATGGGATTGCGCTGCTTCTAACCGTCCTTCACTGGACAATACAAATGACAATTTGACCTCAAATCAGGTAGGACTACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACTAACAAGGATTCCCCTAGTAACTGCGAGTGAAGCGGGAAAAGCTCAAATTTAAAATCTGGCGGTCTCTGGCCGTCCGAGTTGTAATCTAGAGAAGTGTTATCCGCGCTGGACgt

Surprisingly, it differs from the ITS sequence of P. cf. tasmaniana from the Heyowana collection by only one base pair.  This isn't the real P. tasmaniana, I am calling it Psilocybe cf. tasmaniana for now.

It's pretty close to P. baeocystis and P. stuntzii, and not too far from P. semilanceata.



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OfflineAlan RockefellerM
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Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Alan Rockefeller]
    #23381404 - 06/25/16 04:41 PM (7 years, 8 months ago)

A new phylogenetic tree, which includes this collection and a bagels arrow sign shamelessly copied from google images.


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InvisibleBagels
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Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Alan Rockefeller]
    #23381466 - 06/25/16 05:00 PM (7 years, 8 months ago)

This is stretching my brain cells way too much but thank you Alan for the work that you have done. I look forward to others comments :cool:

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InvisibleMatai


Registered: 05/04/14
Posts: 1,016
Loc: NZ Flag
Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Bagels]
    #23381781 - 06/25/16 06:47 PM (7 years, 8 months ago)

Wow, this is awesome to see. Is a single base pair within the error margins of DNA sequencing? Or is it statistically significant?


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OfflineAlan RockefellerM
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Re: ID Request for a small mushroom that looks like a Psilocybe sp. NZ [Re: Matai]
    #23382250 - 06/25/16 09:19 PM (7 years, 8 months ago)

Quote:

Matai said:
Wow, this is awesome to see. Is a single base pair within the error margins of DNA sequencing? Or is it statistically significant?





It is within the error margins - the TAQ polymerase makes errors at the rate of roughly 1 in 1000.    If I sequenced more times and more collections, I could be more sure that it's an accurate result.

In most cases even if it is accurate, 1 base pair by itself is not enough to delimit a species.  But if other characteristics are also different, it could be.

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