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Offlineangryjslice
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Super easy karo tek * 6
    #2321056 - 02/10/04 12:13 PM (20 years, 10 days ago)

Do you have a microwave? Tired of messing up w/ your karo teks?

heres your solution:

supplies your gonna need:

1 package of small microwavable containers - like these:



1 bottle cheap imitation light syrup - like this:



1 package cheap plastic wrap - like this:



total cost should be under $5

ok now heres what you do:

1) pour about 200ml's of water (around 5 ounces) into the container.

2) add a teaspoon of light syrup to the water(im sure more could be used to increase results, but this is just what i did)

3) put a peice of plastic wrap over the top of the container, and then put the lid on top of that - like this:



4) microwave the container, with the lid slightly cracked (so the container doesnt melt) for 5 Minutes . when its done nuking, open your microwave and push the lid down so its all sealed in there (carefull, its hot)

5) let cool for a few hours, or untill it is room temperature again, and then lift up the lid slowly, flame sterilize your syringe, and put in 1 - 2cc's of your left over spore solution.

6) toss these guys in your incubator (im using a double tub setup)



7) after a few days you should start to see some myc. growth in the solution. after about a week and a half your container should look like this.



i shake the containers around every few days and let the water sloshing break up the myc. no need for glass chunks.

when your ready to innoculate, just lift up the lid, and poke the needle of your sterile syringe through the plastic wrap and suck up how much ever juice you need.

congratulations...you just spent $5 to turn 2 cc's of spore solution into 200 cc's of spore solution. easy as pie in a microwave too.


well i hope this helps someone. enjoy everybody.

~JSlice~


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OfflineSillllyFressshhhh
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Re: Super easy karo tek [Re: angryjslice]
    #2321080 - 02/10/04 12:22 PM (20 years, 10 days ago)

WOW Sounds fantastic I think Ima try this tonight once I get home from work, THANKS!


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Invisibleniteowl
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Re: Super easy karo tek [Re: angryjslice]
    #2321129 - 02/10/04 12:40 PM (20 years, 10 days ago)

I have done the same thing with half pint jars. Wrap the plastic over the top and nuke. When done nuking put the lids w/hole on, and incubate.
When they are ready just poke the needle thru the hole and suck up.


--------------------
Live for the moment you are in now
Don't be bogged down by your past
Don't be afraid of what lies in your future


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OfflineBen_Stein
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Re: Super easy karo tek [Re: SillllyFressshhhh]
    #2321234 - 02/10/04 01:14 PM (20 years, 10 days ago)

Quote:

SillllyFressshhhh said:
WOW Sounds fantastic I think Ima try this tonight once I get home from work, THANKS!




Shit!  I'm gonna quit my job, go home and try it...NOW.  :lol:

Nice tip, jslice...tanks  :thumbup:

I would consider using two teaspoons of syrup to 200ml, ,though, but it shonuf worked.


--------------------
I'm so ready to start growing. I've shroomed twice and loved it to no extent. --Mindgames


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OfflineGr0wer
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Re: Super easy karo tek [Re: angryjslice]
    #2321418 - 02/10/04 02:03 PM (20 years, 10 days ago)

Is that last pic what a fully colonized solution looks like?


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Offlineangryjslice
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Re: Super easy karo tek [Re: Gr0wer]
    #2321681 - 02/10/04 03:21 PM (20 years, 10 days ago)

that last picture is when ive taken it out of the incubator at this point...its colonized enough to the point that i can make 20 syringes out of it.

~JSlice~


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OfflineMagicBrownie
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Re: Super easy karo tek [Re: angryjslice]
    #2322055 - 02/10/04 08:38 PM (20 years, 10 days ago)

Why does this seem too good to be true?

Does this really work?

Is light syrup the same as corn syrup?

Is it actually sucking spores into the syringe, or mycelium?


--------------------
"From one shroom comes a million."
The Shroomacologist


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InvisibleYarry
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Re: Super easy karo tek [Re: MagicBrownie]
    #2322073 - 02/10/04 08:42 PM (20 years, 10 days ago)

Yeah it works, i did the same with a little molasses. and itll be myc in your syringe not spores. so not legal to trade.. jus wanna make sure everyone knows that. dont want to get yourself in trouble


--------------------
Grumpy Old Man.


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OfflineMagicBrownie
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Re: Super easy karo tek [Re: Yarry]
    #2322091 - 02/10/04 08:44 PM (20 years, 10 days ago)

How long can i wait to inoculate a jar with the syringe?

Will it be just as fast and effective as spores?


--------------------
"From one shroom comes a million."
The Shroomacologist


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OfflineBen_Stein
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Re: Super easy karo tek [Re: MagicBrownie]
    #2322118 - 02/10/04 08:48 PM (20 years, 10 days ago)

It's lots faster cuz the spores are already germinated, cutting out at least five days.

Also, it seems to grow faster in general, plus you no longer have to be stingy with your apores.


--------------------
I'm so ready to start growing. I've shroomed twice and loved it to no extent. --Mindgames


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OfflineMagicBrownie
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Re: Super easy karo tek [Re: Ben_Stein]
    #2322131 - 02/10/04 08:51 PM (20 years, 10 days ago)

This is the best news ive heard in a long time!

i only have 3 cc's of solution left in my syringe, and i cant wait to give this a try!


--------------------
"From one shroom comes a million."
The Shroomacologist


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Offlineangryjslice
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Re: Super easy karo tek [Re: MagicBrownie]
    #2322213 - 02/10/04 09:05 PM (20 years, 10 days ago)

using liquid culture ive colonized half pints completly in 5 days. this isnt anything new that hasnt been done before, but i havnt ever seen a pictorial or instructions for karo in the microwave.

~JSlice~


--------------------


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OfflineGr0wer
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Re: Super easy karo tek [Re: Yarry]
    #2322281 - 02/10/04 09:16 PM (20 years, 10 days ago)

Quote:

YarryShroomer said:
Yeah it works, i did the same with a little molasses.




How well did the molasses work? Was it normal dark molasses?


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InvisibleYarry
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Re: Super easy karo tek [Re: Gr0wer]
    #2322301 - 02/10/04 09:19 PM (20 years, 10 days ago)

Yeah normal dark molasses, it worked pretty well, same amount of myc just a little harder to see cuz its darker. i didnt really do any measurements, just filled a half pint jar with water and added molasses til it looked slightly darker then whiskey. tossed on saran wrap and a plastic lid and microwaved er


--------------------
Grumpy Old Man.


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OfflineNickster_154371
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Re: Super easy karo tek [Re: Yarry]
    #2335240 - 02/14/04 06:01 PM (20 years, 6 days ago)

When your incubating the karo container do you need to have holes punched in it for gas exchange?


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Invisiblesimplemachine
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Re: Super easy karo tek [Re: Nickster_154371]
    #2335288 - 02/14/04 06:18 PM (20 years, 6 days ago)

^^^no it should be as well sealed as possible.


SM


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OfflineBen_Stein
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Re: Super easy karo tek [Re: simplemachine]
    #2335562 - 02/14/04 08:09 PM (20 years, 6 days ago)

^^^ yeah, sealed tight. The culture will live for a few months off the air already in there, which is really nice.


--------------------
I'm so ready to start growing. I've shroomed twice and loved it to no extent. --Mindgames


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Invisiblegugglebum
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Re: Super easy karo tek [Re: Ben_Stein]
    #2336714 - 02/15/04 08:15 AM (20 years, 6 days ago)

Aren't the Shroomery sponsors going to be really pissed off at whoever created the Karo Tek? Oh well ... hehehe


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OfflineBen_Stein
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Re: Super easy karo tek [Re: gugglebum]
    #2336772 - 02/15/04 08:57 AM (20 years, 6 days ago)

Quote:

gugglebum said:
Aren't the Shroomery sponsors going to be really pissed off at whoever created the Karo Tek? Oh well ... hehehe




The tek prolly has been around longer than most of them.

Unfortunately, it's the nature of the business (the spore part, anyway) that you may or may not see a lot of repeat business.

Happily, most of the sponsors seem to be holding their own.


--------------------
I'm so ready to start growing. I've shroomed twice and loved it to no extent. --Mindgames


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OfflineBruce3
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Re: Super easy karo tek [Re: angryjslice]
    #2337017 - 02/15/04 11:08 AM (20 years, 5 days ago)

do you poke through the plastic wrap when injecting the spores? or do you lift it up?


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OfflineAkrobatik
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Re: Super easy karo tek [Re: Bruce3]
    #2337049 - 02/15/04 11:21 AM (20 years, 5 days ago)

poke def, you dont want to expose the myc to any air unless you have a glove box or somthing similar.
:mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2:


--------------------
:mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2: :mushroom2:


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InvisiblePsychoslut
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Re: Super easy karo tek [Re: angryjslice]
    #2337737 - 02/15/04 02:38 PM (20 years, 5 days ago)

great, but i think your thinking a little too far into it, it does not need to be this complicated, with all the surran wrap and junk, just have the lid on with an innoc hole, see the steam in the container is going to cause a positive pressure environment in the container, not allowing dirty air to enter the hole, long enough for you to throw on a piece of tape before it cools, then just innoc like ya would with a half pint when its cooled, really all this tek did is eliminate the metal lid on a jar, im not bashing you at all though, its cool.

4) microwave the container, with the lid slightly cracked(so the container doesnt melt) for 5 Minutes . when its done nuking, open your microwave and push the lid down so its all sealed in there (carefull, its hot)

it wont melt, all of the plastic storage containers are microwave and freezer safe these days, you just need a vent for steam so the lid doesnt pop off, wich is why you just make your innoc hole in the lid before microwaving, sterilize it, take it out of the nuker, and throw on some tape over the hole asap.


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


Edited by Psychoslut (02/15/04 02:59 PM)


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OfflineNickster_154371
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Re: Super easy karo tek [Re: Bruce3]
    #2338183 - 02/15/04 04:40 PM (20 years, 5 days ago)

poke through


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OfflineTinTree
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Re: Super easy karo tek [Re: Nickster_154371]
    #2338287 - 02/15/04 05:15 PM (20 years, 5 days ago)

Somebody asked earlier, and I'm curious too.
How long can you store the fluid before inoculation?
(First post here btw... hi everybody!)


--------------------
"I'm afraid of losing my obscurity. Genuineness only thrives in the dark. Like celery."
- Aldous Huxley


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Invisiblesimplemachine
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Re: Super easy karo tek [Re: TinTree]
    #2338325 - 02/15/04 05:29 PM (20 years, 5 days ago)

I've stored LC jars for weeks before innoculating.


SM


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Invisiblesimplemachine
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Re: Super easy karo tek [Re: angryjslice]
    #2338344 - 02/15/04 05:37 PM (20 years, 5 days ago)

nice work j...

this might make liquid culture a bit more n00b friendly. The only thing I would suggest is the addition of some peroxide.

AFTER the spores have germinated several cc's of peroxide (about 5 or so for that much water) should be addded through the innoculation hole. The peroxide prevents contams and speeds up colonization by adding 02 to the karo solution.


sM


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Offlinevermonster
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Re: Super easy karo tek [Re: simplemachine]
    #2338587 - 02/15/04 07:10 PM (20 years, 5 days ago)

Afoaf has used a 1/2 pt jar with a plastic lid for the same
idea. She has to heat it for 1 min and then let it cool for
a bit and heat it again. If she does it for 5 min straight
the innoc hole is too small too relieve excess pressure and
the top pops off. She also covers it with a coffee filter for
heating and then tyvek for incubation. For storage the jar is
covered in foil and put in the door of the fridge (shakes the jar a little).


--------------------


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Offlineprstnjak
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Re: Super easy karo tek [Re: Bruce3]
    #2338782 - 02/15/04 08:03 PM (20 years, 5 days ago)

does anybody know any other brand of imitation light syrup???

And where I can get it Thanks!


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Offlinesalazare
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Re: Super easy karo tek [Re: prstnjak]
    #2340583 - 02/16/04 10:20 AM (20 years, 5 days ago)

HI this is realy usefull and easy, thanks guys
one simple question thought, how long can this thing be good if kept to room temperature?


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Offlinesalazare
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Re: Super easy karo tek [Re: salazare]
    #2342163 - 02/16/04 04:13 PM (20 years, 4 days ago)

bump


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OfflineNickster_154371
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Re: Super easy karo tek [Re: simplemachine]
    #2343641 - 02/16/04 10:07 PM (20 years, 4 days ago)

Simplemachine, how do you know when the spores germinate? I want to inject a few cc's of peroxide to help speed things up, but im not sure what im looking for. Also, its been about 2 days since i innoced w/ spores and nothing seems to be happening...i just have a big clump of black spores floating around in the liquid and its being kept at 85-87 degreez.


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Invisiblesimplemachine
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Re: Super easy karo tek [Re: Nickster_154371]
    #2343694 - 02/16/04 10:18 PM (20 years, 4 days ago)

I would wait untill there is at least a little myc colonizing the jar. after maybe a week.


SM


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Offlinesalazare
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Re: Super easy karo tek [Re: simplemachine]
    #2349207 - 02/18/04 08:19 AM (20 years, 3 days ago)

someone can help me about how long is this karo mycelium solution viable if stored at room temperature?


Edited by salazare (02/18/04 08:21 AM)


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Invisiblesimplemachine
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Re: Super easy karo tek [Re: salazare]
    #2349285 - 02/18/04 08:53 AM (20 years, 3 days ago)



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Offlinemavix
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Re: Super easy karo tek [Re: angryjslice]
    #2349315 - 02/18/04 09:03 AM (20 years, 3 days ago)

As quoted below, I'm wondering the following, you stated that the lid is cracked open while cooking, yet it gets sealed imediately after the microwave has stopped. how does one do this without introducing contaminated air....

by following your instructions i had the following effect, I sealed the tupperware while hot. the resulting cooling of the expanded gasses inside created a vacuum.... this was then released when puncturing the saran wrap. how does one do this w/o introducing contaminants. do you do the innoculation and subsequent solution removal in your GB?

Mavix who is worried about contamns.

Quote:

angryjslice said:

3) put a peice of plastic wrap over the top of the container, and then put the lid on top of that - like this:

4) microwave the container, with the lid slightly cracked (so the container doesnt melt) for 5 Minutes . when its done nuking, open your microwave and push the lid down so its all sealed in there (carefull, its hot)

5) let cool for a few hours, or untill it is room temperature again, and then lift up the lid slowly, flame sterilize your syringe, and put in 1 - 2cc's of your left over spore solution.





--------------------
-- mavix


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InvisiblePsychoslut
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Re: Super easy karo tek [Re: mavix]
    #2350070 - 02/18/04 12:26 PM (20 years, 2 days ago)

read my post above


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Offlineangryjslice
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Re: Super easy karo tek [Re: mavix]
    #2350090 - 02/18/04 12:29 PM (20 years, 2 days ago)

the lid is cracked so that the container doesnt melt, and so the plastic wrap doesnt melt into the lid. i had no trouble at all with any contaminations even though air filled back in when i injected the spore solution. ive done this many times. if your even in a semi sterile area it shouldnt be a problem. make sure you sterilize your needle though.

~JSlice~


--------------------


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InvisiblePsychoslut
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Re: Super easy karo tek [Re: angryjslice]
    #2350123 - 02/18/04 12:35 PM (20 years, 2 days ago)

dude if you use a microwave safe dish it will not melt, im not sure about surran wrap, but the surran wrap is not needed, explain to me why you couldnt do this exactly the way you would a half pint with a plastic lid, besides the steam popping the lid off, and i posted the solution for that already.


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Offlineangryjslice
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Re: Super easy karo tek [Re: Psychoslut]
    #2350125 - 02/18/04 12:35 PM (20 years, 2 days ago)

>> really all this tek did is eliminate the metal lid on a jar, im not bashing you at all though, its cool.

actually all this tek did was put pictures and a basic outline to something we had been discussing for a while that nobody had compiled into one easy to follow outline. this is my personal way of doing it.  just wanted to make it easy, not 'cool'.  the plastic wrap is very usefull because it acts as another contam barrier.  sure you could go without it, but why?  then you have to deal with making a resealable hole in the lid of your container.


>>  it wont melt, all of the plastic storage containers are microwave and freezer safe these days

the containers i have say right on them "vent lid during microwaving to prevent melting" ...there the cheap ones and yes i did try to leave the lid closed during a different experiment with the same containers and the plastic caught on fire :nonono:

sorry for the double post

~JSLice~


--------------------


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Offlineangryjslice
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Re: Super easy karo tek [Re: Psychoslut]
    #2350149 - 02/18/04 12:39 PM (20 years, 2 days ago)

Quote:

Psychoslut said:
dude if you use a microwave safe dish it will not melt, im not sure about surran wrap, but the surran wrap is not needed, explain to me why you couldnt do this exactly the way you would a half pint with a plastic lid, besides the steam popping the lid off, and i posted the solution for that already.




use your half pints...personally, all of my half pints were in use and i needed more solution for innoc. to each there own man. its just another idea, several people have pm'd me and said this was very easy and worked out well for them.

~JSlice~


--------------------


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InvisiblePsychoslut
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Re: Super easy karo tek [Re: angryjslice]
    #2350150 - 02/18/04 12:40 PM (20 years, 2 days ago)

you will be using your pre-made innoculation hole to vent the lid while it is microwaving, then take it out of the microwave, throw a piece of masking tape on there, wait for it to cool, and innoc it like you would anything.


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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InvisiblePsychoslut
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Re: Super easy karo tek [Re: angryjslice]
    #2350163 - 02/18/04 12:42 PM (20 years, 2 days ago)

you are reading and responding to my posts too quickly, your not understanding what im saying, slow do killer.


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[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Offlineangryjslice
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Re: Super easy karo tek [Re: Psychoslut]
    #2350171 - 02/18/04 12:43 PM (20 years, 2 days ago)

i didnt realize they sold a package of 6 mini plastic containers that come with innoculation holes in them. please point me to them. this is all out of the box and easy to do. if you dont like it dont use it.

the plastic wrap is only so you can lift the lid up and innoc through it while cutting down on potential contams from landing in your solution.

~JSlice~


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Offlineshangrila
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Re: Super easy karo tek [Re: Bruce3]
    #2350179 - 02/18/04 12:44 PM (20 years, 2 days ago)

its an incredible idea, but tell me about incubation please


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OfflineNewSpore
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Re: Super easy karo tek [Re: angryjslice]
    #2350187 - 02/18/04 12:46 PM (20 years, 2 days ago)

Hey J,

Nice job. Makes it simple.

Thanks for sharing and taking the time


--------------------
Be in truth and watch the magic happen.
SBP TEK


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InvisiblePsychoslut
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Re: Super easy karo tek [Re: angryjslice]
    #2350198 - 02/18/04 12:48 PM (20 years, 2 days ago)

why would it be more difficult to poke a hole in a plastic lid then in a metal half pint one? now your just getting angry and will not listen to reason, so i will be leaving now.


--------------------



[quote]KristiMidocean said:
Good now thats clear.WHO FUCKING CARES. If I am fat u all keep pointing it out like its suppose to be a secret.LIke u really have nothing better to do then make fat jokes. If o know its like I do I know yall can come up with NEW AND BETTER SHIT . This shit is old and boring . I left in the first place cause this shit got boring not because of the fat jokes . Fat jokes dont bother me but seriously its old[/quote]


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Offlineangryjslice
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Re: Super easy karo tek [Re: Psychoslut]
    #2350680 - 02/18/04 02:18 PM (20 years, 2 days ago)

im sorry psychoslut.

both systems work. i just have found in my personal experience that plastic wrap is easy. i have poked holes in the lids of jars before, i know its not more difficult for metal or plastic...infact it might be easier with plastic because you could just heat up some metal and melt it through instead of using a hammer.

plastic wrap is a quick temporary way of doing this. i see your point with adding a hole in the lid, this way you can use the container over and over no problem. ill give it a try next time i do this system. what kind of tape do you use?


>>its an incredible idea, but tell me about incubation please

after you knock up these containers put em in a dark area between 80 and 86 degrees. within a week you'll have enough liquid culture to do whatever your heart desires.

~JSlice~


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Offlinevermonster
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Re: Super easy karo tek [Re: angryjslice]
    #2380465 - 02/26/04 04:48 PM (19 years, 11 months ago)

My aunt just knocked up 6 1/2pts, 3 syringe and 3 Karo. So far
the Karo looks to be about 5 days ahead of the syringe jars :thumbup:
She's thinking about doubling the recipie next time
for more mycelium. She sucked up all the white stuff and it just
filled a 10cc syringe. Seemed to grow very slow in the jar.


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OfflineP2K1
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Re: Super easy karo tek [Re: vermonster]
    #2380501 - 02/26/04 04:59 PM (19 years, 11 months ago)

Im trying this Tek with 100% Maple Syrup tonight


--------------------
Stay free
:brucelee:
Glenners said:
Epic win P2K1 !
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Mataspore said:
P2K1 is the winn-ar.
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Invisiblehevvy_psi
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Re: Super easy karo tek [Re: P2K1]
    #2380514 - 02/26/04 05:04 PM (19 years, 11 months ago)

wait, so...mycelium will grow in straight syrup?!?!


--------------------
egocentrism is bangin on the door
self-destructive selflessness
seeps out from the core
alone - eyes closed - an empty room
i'm curled on the floor
choose nothing, thus deciding
all the nothing i've in store.


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Invisiblehevvy_psi
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Re: Super easy karo tek [Re: hevvy_psi]
    #2380573 - 02/26/04 05:28 PM (19 years, 11 months ago)

will it ever colonize the solution completely?


--------------------
egocentrism is bangin on the door
self-destructive selflessness
seeps out from the core
alone - eyes closed - an empty room
i'm curled on the floor
choose nothing, thus deciding
all the nothing i've in store.


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InvisibleMoNk
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Re: Super easy karo tek [Re: angryjslice]
    #2380650 - 02/26/04 06:00 PM (19 years, 11 months ago)

I have a few karo jars colonizing, though in the middle of my large puff i have a single black dot, should i be worried about this single black dot?


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Offlinehwackool
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Re: Super easy karo tek [Re: angryjslice]
    #2380980 - 02/26/04 07:56 PM (19 years, 11 months ago)

You are right... it IS super easy..

Thanks for the great info.


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InvisibleTremor1127
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Re: Super easy karo tek [Re: hwackool]
    #2527661 - 04/05/04 01:06 PM (19 years, 10 months ago)

wow... nice...


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Offlineendokrin
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Re: Super easy karo tek [Re: Tremor1127]
    #2528178 - 04/05/04 03:12 PM (19 years, 10 months ago)

Got a question about the syringe- Is it ok to suck up this mycelium through a normal spore syringe, or should a fatter needle be used?
(will it damage the mycelium?)

Thanks


--------------------
"If King Kong sells drugs, we'll put him in jail"


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Offlineangryjslice
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Re: Super easy karo tek [Re: endokrin]
    #2528237 - 04/05/04 03:24 PM (19 years, 10 months ago)

i have had no problems using regular size spore syringes. as long as its sterile, it shouldnt be a problem.

~JSlice~


--------------------


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OfflinePadidleniddle
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Re: Super easy karo tek [Re: angryjslice]
    #2528297 - 04/05/04 03:39 PM (19 years, 10 months ago)

my friend followed these procedures only he put in slightly more syrup, and after nuking the liquid turned a light tint of yellow... is that to be expected?


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OfflineHrethic
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Re: Super easy karo tek [Re: angryjslice]
    #2528478 - 04/05/04 04:40 PM (19 years, 10 months ago)

I made mine with a lot more Karo than you J, and you're right, I don't think it makes it any faster or slower, they can just live in there much longer. I've got one that I inoculated 6 or 7 weeks ago, and it's still producing strands. I had to throw it away because I think I finally saw some contams, but shit, this is the easiest thing in the world to do, and it works splendidly! Thanks J, it's saved me many a dollar, and I know it will the rest of my life, until I find something else to increase one syringe into infinite syringes. :mushroom2:


--------------------
Will all the big boomers please unveil, please unveil, please unveil.


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Offlinedheadport
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Re: Super easy karo tek [Re: Hrethic]
    #4004857 - 04/02/05 12:11 PM (18 years, 10 months ago)

I have two questions...
1) Can you introduce live tissue to this tech. Say you have 1 cake and no spores. Break the corner open in a glovebox and scrap some tissue in?
2) How does the my get into the syringe, or is it the process of shaking up the container and then drawing solution> So you are taking up micro lovels of the lliving tissue?


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OfflinekronnyQ
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Re: Super easy karo tek [Re: dheadport]
    #4004904 - 04/02/05 12:25 PM (18 years, 10 months ago)

Props to angry for this one.

I always had problems with the microwave because my stuff just wants to boil and pop open.

Was gunna try the baby bottle tek I saw on a diff site but I like this one better, I shall give her a try thx!


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OfflineLiquidMartyr
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Re: Super easy karo tek [Re: angryjslice]
    #4169265 - 05/13/05 01:00 AM (18 years, 9 months ago)

Whatsup everyone. This is my first post on the shroomery. Just want to say that I love everything about this site. It has been extremely helpful.

Let me tell you about this dream I had in regards to the Karo TEK..... I used the Ziplock round, medium "Twist,n lock" containers. I filled them 750 ML of water and about 5 TSP of karo. I used about a square foot of saran wrap when I boiled them (no top). I microwaved them for 6 minutes with no melting problems. When they stopped boiling i put a rubberband around the base of the threads to secure the saran wrap. When they cooled I inoculated one of eatch (they come 2 in a pack... $3.75)with Treasure Coast and Ecuador spores, and then put the tops on. I did it about an hour ago. I guess I will find out in about a week. Hopefully, this will help someone out. Thanks everyone.


--------------------
Yeah, I did that.


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Offlinedown44
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Re: Super easy karo tek [Re: LiquidMartyr]
    #4183452 - 05/16/05 05:56 PM (18 years, 9 months ago)

awesome tek thanks!


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Invisibleshymanta
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Re: Super easy karo tek [Re: Padidleniddle]
    #5125280 - 12/31/05 05:51 AM (18 years, 1 month ago)

Quote:

my friend followed these procedures only he put in slightly more syrup, and after nuking the liquid turned a light tint of yellow... is that to be expected?




This may be the sugars caramelizing. Reduce your heat time. In a pressure cooker it should only be about 30 minute. I don't know about a microwave. Maybe heat more times for a shorter period with a rest in between?


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InvisibleFooMan
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Re: Super easy karo tek [Re: shymanta]
    #5125380 - 12/31/05 07:33 AM (18 years, 1 month ago)

Quote:

shymanta said:
Quote:

my friend followed these procedures only he put in slightly more syrup, and after nuking the liquid turned a light tint of yellow... is that to be expected?




This may be the sugars caramelizing. Reduce your heat time. In a pressure cooker it should only be about 30 minute. I don't know about a microwave. Maybe heat more times for a shorter period with a rest in between?




LOL, Check out the date on that post Shymanta. I would hope after nearly 2 years that they figured the answer out by now!


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OfflineShroomArtist84
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Re: Super easy karo tek [Re: FooMan]
    #5125432 - 12/31/05 08:01 AM (18 years, 1 month ago)

great post man!!!!


--------------------
No matter what I say and no matter what I write here.

I'm sick of always looking at this page with a blank stare.


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Invisibleshymanta
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Re: Super easy karo tek [Re: angryjslice]
    #5125467 - 12/31/05 08:38 AM (18 years, 1 month ago)

Damn, that's funny. Didn't even see the date. I sure hope they figured it out. Maybe someone else will get something from it.


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OfflineSnaggletooth
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Re: Super easy karo tek [Re: shymanta]
    #5126018 - 12/31/05 01:03 PM (18 years, 1 month ago)

shymanta: gets the necromancy award of the day :headbanger:


:bongload:


--------------------


Atheist Chat


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OfflineSpacedone
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Re: Super easy karo tek [Re: P2K1]
    #6263668 - 11/08/06 10:28 PM (17 years, 3 months ago)

a quick question

do you have to suck up the white mycellum itself or will the liquid itself be able to innoc with


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Invisibleoso
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Re: Super easy karo tek [Re: FooMan]
    #6263696 - 11/08/06 10:42 PM (17 years, 3 months ago)

haha.
Quote:

FooManShroom said:

LOL, Check out the date on that post



but yea, you are aiming for the myc, not the sugar water.


--------------------
"When tyranny and oppression come to this land, it will be in the guise of fighting a foreign foe."
James Madison


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Invisiblejack_straw2208
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Re: Super easy karo tek [Re: Spacedone]
    #6643798 - 03/07/07 12:06 AM (16 years, 11 months ago)

Quote:

Spacedone said:
a quick question

do you have to suck up the white mycellum itself or will the liquid itself be able to innoc with




bump


--------------------
If you can’t tell what you desperately need, it’s probably sleep.


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Offlinedjnoktirnal
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Re: Super easy karo tek [Re: jack_straw2208]
    #6646127 - 03/07/07 05:40 PM (16 years, 11 months ago)

Quote:

jack_straw2208 said:
Quote:

Spacedone said:
a quick question

do you have to suck up the white mycellum itself or will the liquid itself be able to innoc with




bump




i would try to suck up myc, although it's probably throughout the water even though you can't see it


--------------------
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InvisibleBlutjager
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Re: Super easy karo tek [Re: djnoktirnal]
    #6646784 - 03/07/07 08:29 PM (16 years, 11 months ago)

YES you need to suck up the mycelium


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Offlinefiggusfiddus
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Re: Super easy karo tek [Re: Blutjager]
    #6647137 - 03/07/07 10:35 PM (16 years, 11 months ago)

Sucking up mycelium isn't hard, if that's what the previous poster was wondering. This is really fluffy stuff, it's only going to even be visible because it's so bloated with water.

I've done simple, successful tissue cultures by just tossing a hunk of stalk in the processor with sterile water, sucking whatever you can up and inoculating. Doing it THAT way will jam up your blunt needle sometimes, but you can just shoot it back out and try again until it works. That's your worst-case scenario with this myc... but I don't think you'll run into that at all.

That said, even the karo water, as long as you let it colonize and then shake it up nicely, is full of tissue and cells. It's just that you get way more tissue if you suck up the actual mycelium. I did experiment a little, by sucking up the really scummy, thick tissue for one jar and just the clearish water for the next, and though the scummy jar got off to a faster start, in the end colonization times don't seem to differ more than one two days (didn't do enough jars in that batch to give a nice scientific average). I figure that has to do with the fact that the watery culture will disperse the tissue over a larger area, making up for some of the lost time, but that's a straight-from-the-ass hypothesis.

Edit: On second thought it should be said that whether you absolutely have to suck up visible mycelium probably has a lot to do with how much it's colonized by the time you start extracting. Like the original poster said though, if you shake it up, you've got 200mL of inoculant (or however much you make), not just the 20mL or so that has visible mycelium in it.


--------------------
FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS
FGSFDSFGSFDSFGSFDSFGSFDSFGSFDS


Edited by figgusfiddus (03/07/07 10:43 PM)


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InvisibleOgla
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Re: Super easy karo tek [Re: figgusfiddus]
    #6647165 - 03/07/07 10:43 PM (16 years, 11 months ago)

wow that a very good one. I was uding the half pint ziplock containers with a burnt hole and electirc tape. seems like a bet.


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Offlinefiggusfiddus
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Re: Super easy karo tek [Re: Ogla]
    #6652456 - 03/09/07 10:52 AM (16 years, 11 months ago)

Just a question about applying this karo tek (or any karo tek) to inoculation with a questionable spore solution:

If I'm not sure my spore solution is very clean, can I apply this tek, then add a little h2o2 before using the karo solution as an inoculant?

As I understand h2o2, it won't hurt live mycelium in small amounts, but it will kill mold spores and bacteria left and right. Thus, by growing live mycelium in a karo solution, it would seem to me you could "clean up" your spores by allowing them to germinate before killing off their competition. I'm pretty sure none of the mold spores can produce advanced enough structures in this time to survive the peroxide holocaust.

Thoughts? I've seen people use h2o2 to clean up messy clones, but I haven't read about anyone using it to effectively clean up bad spores by allowing them to germinate.

Edit: Never mind, I just read this exact same thing in the other karo tek. So uh, I guess I don't get genius credit.


Edited by figgusfiddus (03/11/07 07:31 PM)


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