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Offlinecirilo.h
local mycologist
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Registered: 10/17/15
Posts: 180
Last seen: 5 years, 10 months
Inoculation experiment
    #23206006 - 05/09/16 12:53 PM (8 years, 8 months ago)

I had a idea...
Currently i have 4 cakes that had mycelium on the bottom and cubes already growing out of them  from a cow field lined up in slight weeds/grass up against a fence in my backyard they get indirect sunlight from 7am when the sunrises to about 1pm in the afternoon, then from there till sunset at 8 they get direct sunlight , i spray them with water for a few minutes every other day and they've been doing extremely well, the pins on them are huge and I've picked a few shrooms from them that had really nice size stems for outdoor shrooms,

i live in central florida and the climate right now is great btw,


now my question is i have 4 pp5 trays that i sterilized and i was wondering if i could cut a piece of mycelium off from the bottom of one of the cakes (i have one in mind that is thriving with new mycelium on the bottom) and it it in a iso/purified water mix or just iso to clean it and then , use a syringe to inoculate a tray with it.

i will be posting pics of the cakes and the trays and follow up on it in this thread but id like some advice on it first thanks!

Edited by cirilo.h (05/09/16 12:59 PM)

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Invisibledankington
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Re: Inoculation experiment [Re: cirilo.h]
    #23206297 - 05/09/16 02:33 PM (8 years, 8 months ago)

:justno: You should take a sample from the interior of a fruit instead, and put it on agar. Then you can make an LI once you're sure it's clean and inoculate your prepared grain or whatever.

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InvisibleInocuole
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Re: Inoculation experiment [Re: dankington]
    #23206316 - 05/09/16 02:38 PM (8 years, 8 months ago)

Word "experiment" in title, first reply includes :justno:

Sounds about right.  Definitely don't use anything that has ever come into contact with open air, ever, to inoculate with.


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OfflineSloppyJoseph
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Re: Inoculation experiment [Re: Inocuole]
    #23206325 - 05/09/16 02:41 PM (8 years, 8 months ago)

:justno:


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