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OfflineTHK107
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Are these streaks on my agar normal?
    #23204565 - 05/09/16 01:13 AM (8 years, 8 months ago)

I know that normally if it's not white then it's not right but I had my petri dishes in my bedroom where it gets really hot then I moved them to a different room but before I did so I noticed condensation inside and on top of the agar, I didn't really pay it much attention before I moved it but today when I looked I saw streaks on my plate, I feel like it's a contamination but I really don't want it to be.



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OfflineTHK107
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Re: Are these streaks on my agar normal? [Re: THK107]
    #23204570 - 05/09/16 01:20 AM (8 years, 8 months ago)

Just realized I put this in the wrong forum, sorry guys!

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Invisible13shroomsM
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Re: Are these streaks on my agar normal? (moved) [Re: THK107]
    #23204618 - 05/09/16 01:55 AM (8 years, 8 months ago)

This thread was moved from Mushroom Cultivation.

Reason:
there you go

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InvisibleSupalemonhaze
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Re: Are these streaks on my agar normal? [Re: THK107]
    #23204621 - 05/09/16 01:57 AM (8 years, 8 months ago)

From what I can make out it seem you have bacteria in there. It's no worries, that's what agar is for. Pics aren't that good so I might be mistaken, if it looks like milky white liquid like I suspect, it's bacteria.

Use sandwich tek to clean bacterial transfers, works wonders.

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OfflineTHK107
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Re: Are these streaks on my agar normal? [Re: Supalemonhaze]
    #23204661 - 05/09/16 02:35 AM (8 years, 8 months ago)

Quote:

Supalemonhaze said:
From what I can make out it seem you have bacteria in there. It's no worries, that's what agar is for. Pics aren't that good so I might be mistaken, if it looks like milky white liquid like I suspect, it's bacteria.

Use sandwich tek to clean bacterial transfers, works wonders.




On the one where it is small and surrounds the mycelium I think I may have streaked the x acto blade across it trying to get mycelium off the blade.  I'll still sandwich tek it tomorrow night.

The other one has it all over the agar plate even the far edges, it looks like it has dots on it to which is why I thought it may have been condensation.

EDIT: I saw RR recommend a hot agar pour, this sounds like the agar sandwich but easier, I'll be trying it tomorrow night.

Edited by THK107 (05/09/16 03:23 AM)

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InvisibleSupalemonhaze
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Re: Are these streaks on my agar normal? [Re: THK107]
    #23206109 - 05/09/16 01:33 PM (8 years, 8 months ago)

Hot pour is different, mostly used for molds. Sandwiching will get rid of bacteria with 1 transfer if done right. There shouldn't be any doubt when you have bacteria, it looks literally like thick milk. I couldn't make anything out because of the pics but they are really easy to spot. Mycelium will have very small strands at the edges, bacteria is just a blob.

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