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shanesean023
NewGuy

Registered: 04/29/16
Posts: 117
Last seen: 7 years, 10 months
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LC problem?
#23203348 - 05/08/16 06:41 PM (8 years, 8 months ago) |
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4 jars started a week ago and no signs of any growth. South American spore syringe from freespores, 250 ml of water and 1 tablespoon of honey in a pint jar, one 1/4 inch hole with tyvek cover over on the lid and under the ring; pc for 15 minutes at 15 lbs. let the jars cool in PC overnight, took jars straight out of the PC and put in GB stored at 85°, stir marble in two of the jars. Inoculated inside GB with 1cc per jar, flame sterilized the needle. Wore clean clothes, gloves, mask, hair net, sprayed the whole room three times and no air flow. Why no growth? This is the third time without success
-------------------- Looking for a good Mexicana species, I have alot to trade.
This account is strictly for role-playing purposes! Nothing that is said, posted, mentioned, or messaged is fact based and has never actually happened. Thanks for playing along.
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SloppyJoseph
Non-sporalator


Registered: 12/08/15
Posts: 8,511
Loc:
Last seen: 4 years, 7 months
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When you use spores to start an LC, sometimes they will need time to germinate. It's definitely the longest method of making an LC start to finish. Be patient, You might not even see germination still next week.
-------------------- AMU Q&A
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tripdawg420
low life with no life



Registered: 02/02/09
Posts: 7,653
Loc: illinois
Last seen: 14 hours, 38 minutes
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lc isnt the best way to start spores tyveck under the lid stored at 85 all not good i wouldnt mess with lc learn agar its just as easy and way better
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shanesean023
NewGuy

Registered: 04/29/16
Posts: 117
Last seen: 7 years, 10 months
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The tyvek is ontop of the solid part of the lid and under the screw on part. Should I take it off? Is 85° to hot? I want to go with agar but I want to make sure these spores are good or at least grow something before I put anymore money into this hobby. Over $500 in the hole so far :/
-------------------- Looking for a good Mexicana species, I have alot to trade.
This account is strictly for role-playing purposes! Nothing that is said, posted, mentioned, or messaged is fact based and has never actually happened. Thanks for playing along.
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SloppyJoseph
Non-sporalator


Registered: 12/08/15
Posts: 8,511
Loc:
Last seen: 4 years, 7 months
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Unless you bought an All American and built your own flow hood, sounds like you are seriously over budget spending stupid money.
If you have a pressure cooker, agar is another 15$ investment. Best money you'll spend in this hobby. It will pay for itself with healthy shrooms and better yields.
-------------------- AMU Q&A
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shanesean023
NewGuy

Registered: 04/29/16
Posts: 117
Last seen: 7 years, 10 months
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Ive tried 40+ jars and no growth at all what so ever. Not even a contamination. . . at least then I would have successfully grown somethin ha. Thank you guys for the quick replies
-------------------- Looking for a good Mexicana species, I have alot to trade.
This account is strictly for role-playing purposes! Nothing that is said, posted, mentioned, or messaged is fact based and has never actually happened. Thanks for playing along.
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shanesean023
NewGuy

Registered: 04/29/16
Posts: 117
Last seen: 7 years, 10 months
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Should I get the telephone agar?
-------------------- Looking for a good Mexicana species, I have alot to trade.
This account is strictly for role-playing purposes! Nothing that is said, posted, mentioned, or messaged is fact based and has never actually happened. Thanks for playing along.
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Josex
#cheat_code



Registered: 11/13/15
Posts: 8,999
Loc:
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This musltispore to LC thing pops up a lot, so why not just quote something that was discussed today already?...
Quote:
Josex said:
Quote:
NDStepp84 said: Not sure if it's your first grow, but a lot of people starting out have the misconception that it would be faster and easier to start out with LC, when in fact if done right it is one of the most advanced techniques there is, would take way more time and not worth dealing with unless you are inoculating 50 quarts or so IMO.
To be done right you would need to germinate spores on agar, transfer a few times to make sure you have a clean inoculant, use a wedge to inoculate the LC, wait for the LC to colonize, then back to agar to make sure it's clean (more waiting). Spores directly to an LC would be a big gamble and most likely be more of a waste of time than doing it right.
No spore syringe is 100% clean and you cannot look at an LC and tell if it is good or bad. I didn't click it but I'm assuming dank linked pastys agar tek, super simple and easy. Tiger drop or TD tic tac toe to grains after you have clean plates FTW.
 I just want to add that if you plan to inoculate a LC with MS, it only takes the tiniest amount of bacteria getting into the liquid media to have a shitexplosion of them in no time and the worst thing is that you'd probably never know until it's too late and you used that LC to inoculate some projects.
Syringes are never sterile, they can be very aseptic and that's as good as it gets, meaning that it's highly probable a syringe may have bacteria. Bacteria will grow and expand at an exponential rate in the liquid media.
There are zillions of terrible and outdated teks that tell you to inoculate LC with a MS syringe. Syringes are not clean enough for this and spores will take their sweet time to germinate and grow out, thus defeating the purpose of making this otherwise awesome inoculant, namely insane speed of colonization of the substrate you inoculate with it.
LC is a really advanced tek that should only be performed by experienced growers, and even they struggle with it sometimes. You should also test your LC on agar every time you want to use it, just to check that it's clean.
Also, in order to make a perfect LC you'd need the right equipment and set of skills: -Agar is a must and you'd need to be proficient at it to do LC's. -Magnetic stirrer to increase the speed of colonization of the LC. -Watman filters and proper SHIP's. -Maybe an Eberbach container to blend a wedge from agar. -Malt extract instead of honey.
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SloppyJoseph
Non-sporalator


Registered: 12/08/15
Posts: 8,511
Loc:
Last seen: 4 years, 7 months
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Quote:
shanesean023 said: Ive tried 40+ jars and no growth at all what so ever. Not even a contamination. . . at least then I would have successfully grown somethin ha. Thank you guys for the quick replies
Are all those 40+ jars being tried with the same syringe? If you didn't buy your syringe from a shroomery sponsor, then you might have honestly been sold water in a syringe, with that much of a failure rate. Even though MS LC isn't recommended, it still should work.
-------------------- AMU Q&A
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shanesean023
NewGuy

Registered: 04/29/16
Posts: 117
Last seen: 7 years, 10 months
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No used different syringes at times; ten different batches at 4 quart jars each time onto WBS. I got 9 different strains from our sponsor freespores. I checked each syringe for little black specs and found them in all but 1 syringe.
-------------------- Looking for a good Mexicana species, I have alot to trade.
This account is strictly for role-playing purposes! Nothing that is said, posted, mentioned, or messaged is fact based and has never actually happened. Thanks for playing along.
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Supalemonhaze
Spore syringe hater.



Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
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Quote:
SloppyJoseph said: Unless you bought an All American and built your own flow hood, sounds like you are seriously over budget spending stupid money.
If you have a pressure cooker, agar is another 15$ investment. Best money you'll spend in this hobby. It will pay for itself with healthy shrooms and better yields.
Not everyone has it easy in this hobby. I spent about 500 euro for my presto, jars and all the little shit you need to start out. Definitely spent over 1000 euro so far, probably around 1300-1500 if I had to guess.
But yeah, I agree. You should not drop spores into an LC unless you are only looking for germination and transferring to agar right after. Especially with a print/syringe from freespore rings. Those are made by hobby-ists.
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