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InvisibleLeftfield420
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transfer questions
    #23201989 - 05/08/16 11:12 AM (8 years, 8 months ago)

All the pastyplates I made up now have tomentose mycelium growing in them.... my first question is: is this normal from spore inoculated agar? My 2nd question is: how do I identify sectors in tomentose myc? My final question is: how do I promote rhizomorphic mycelium on agar so that I can identify sectors?

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Invisibledankington
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Re: transfer questions [Re: Leftfield420]
    #23202013 - 05/08/16 11:20 AM (8 years, 8 months ago)

It could appear tomentose simply because of so many genetics, it could also be bacteria, or even having a very nutritious agar.

So, first question did you use a lot of spores? If so, just just make transfers to see what you get. It may take a few.

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Offlinetripdawg420
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Re: transfer questions [Re: dankington]
    #23202023 - 05/08/16 11:22 AM (8 years, 8 months ago)

right just start xfering :takingnotes:


--------------------
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How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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InvisibleLeftfield420
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Re: transfer questions [Re: dankington]
    #23202025 - 05/08/16 11:23 AM (8 years, 8 months ago)

I believe it's genetics, the cake that the fruit I printed was on had tomentose myc too

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InvisibleLeftfield420
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Re: transfer questions [Re: dankington]
    #23202029 - 05/08/16 11:25 AM (8 years, 8 months ago)

No just a very tiny bit, the clump of spores I scraped off was about the size of a flea

Edited by Leftfield420 (05/08/16 11:25 AM)

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Offlinetripdawg420
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Re: transfer questions [Re: Leftfield420]
    #23202031 - 05/08/16 11:26 AM (8 years, 8 months ago)

thats still alot :crazy2:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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InvisibleLeftfield420
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Re: transfer questions [Re: dankington]
    #23202037 - 05/08/16 11:27 AM (8 years, 8 months ago)

I used the agar recipe from Pastywhyte...with a tad bit more agar powder for firmer agar

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Invisibledankington
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Re: transfer questions [Re: tripdawg420]
    #23202040 - 05/08/16 11:27 AM (8 years, 8 months ago)

:whathesaid:
That's what happened. You are going to need a lot of transfers then.

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InvisibleLeftfield420
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Re: transfer questions [Re: dankington]
    #23202054 - 05/08/16 11:31 AM (8 years, 8 months ago)

Good to know next time I'll use way less, it was my first time using agar

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Invisibledankington
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Re: transfer questions [Re: Leftfield420]
    #23202078 - 05/08/16 11:38 AM (8 years, 8 months ago)

Yep. It's a process. It's hard because you can't see the spores with the naked eye. But it just takes some practice.

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InvisibleJosex
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Re: transfer questions [Re: dankington]
    #23202106 - 05/08/16 11:44 AM (8 years, 8 months ago)

You normally start to see sectoring after quite a few transfers from MS, it can take 5 if you're lucky or even more than 10.

Also, don't worry about tomentose myc. Myc will become rhizo if the conditions change.

Just transfer some samples from the leading edge of that MS plate to new ones, taking that the myc looks healthy.

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InvisibleLeftfield420
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Re: transfer questions [Re: Josex]
    #23202118 - 05/08/16 11:49 AM (8 years, 8 months ago)

It does look nice and white....I'll keep taking transfers and using the agar sandwhich tek so I can clean them up if need be....

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Offlinepuka
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Re: transfer questions [Re: Josex]
    #23202133 - 05/08/16 11:53 AM (8 years, 8 months ago)

Can you wait too long to transfer?

I have 15 pastyplates that are about 3 weeks old now.
Can I use a 3" blunt tip 14 gauge needle as my scalpel?

Flame sterilized ofc. It's all I have right now. You only need like a mm piece to transfer correct?

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InvisibleLeftfield420
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Re: transfer questions [Re: Leftfield420]
    #23202137 - 05/08/16 11:55 AM (8 years, 8 months ago)

Thanks for the input everyone....you guys are great! The info definently helps a noob out!

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InvisibleLeftfield420
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Re: transfer questions [Re: puka]
    #23202143 - 05/08/16 11:57 AM (8 years, 8 months ago)

I was told it was best to wait until the myc was tge size of a quarter, I don't know if that helps you out any bro?

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Offlinetripdawg420
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Re: transfer questions [Re: Leftfield420]
    #23202162 - 05/08/16 12:01 PM (8 years, 8 months ago)

right any size will do i like mine a lil bigger so i get more xfers


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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Offlinepuka
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Re: transfer questions [Re: tripdawg420]
    #23202191 - 05/08/16 12:10 PM (8 years, 8 months ago)

Ok yeah mine are almost the size of the whole container, definitely bigger than a quarter. Time to get to work!

Thanks brotha, good luck to ya

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Invisiblemupetmower
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Re: transfer questions [Re: puka]
    #23202194 - 05/08/16 12:11 PM (8 years, 8 months ago)

heh, i let a few of mine wait a little too long as well. making the agar tonight and transferring a ton in the morning. =]

was going to do it a few days ago, but i got sick blehh


--------------------
-The wise man never stops seeking knowledge.


-I wanna feel the change consume me, feel the outside turning in. I wanna feel the metamorphosis and cleansing I've endured within.

Edited by mupetmower (05/08/16 12:12 PM)

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InvisibleJosex
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Re: transfer questions [Re: Leftfield420]
    #23202216 - 05/08/16 12:18 PM (8 years, 8 months ago)

Quote:

Leftfield420 said:
It does look nice and white....I'll keep taking transfers and using the agar sandwhich tek so I can clean them up if need be....




No need to sandwich it. The sandwich tek is normally used to clone wild fruits and when you're dealing with heavy bacterial contamination or even if you need to germinate some stubborn spores.

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InvisibleLeftfield420
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Re: transfer questions [Re: Josex]
    #23202332 - 05/08/16 01:06 PM (8 years, 8 months ago)

Ok thanks man

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