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Offlinetump
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Registered: 03/17/16
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if you make lc with un modified lid question.
    #23200842 - 05/08/16 12:43 AM (8 years, 8 months ago)

How do you clean the outside of syringe to not be a contam risk when sucking up the lc to inject the other jars of grains in glovebox

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OfflinePsilosoulful

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Re: if you make lc with un modified lid question. [Re: tump]
    #23200918 - 05/08/16 01:19 AM (8 years, 8 months ago)

You don't "clean" it, you have to flame sterilize the entire needle before inserting into your sterile LC jar

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InvisibleSupalemonhaze
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Re: if you make lc with un modified lid question. [Re: Psilosoulful]
    #23200943 - 05/08/16 01:31 AM (8 years, 8 months ago)

I think he means the plastic of the syringe. If I understand correctly, he is going to use an unmodified lid so he is going to have to reach in with his syringe inside his jar.

But still, you don't have to OP. You can tilt the jar so only the needle goes inside or just pour it in your jars since it's gonna be already open. It would be less risky to pour IMO, you're gonna spend a lot of time with the LC without a lid if you are sucking the LC up with a syringe so it would be faster and thus safer to pour.

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OfflinePsilosoulful

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Re: if you make lc with un modified lid question. [Re: Supalemonhaze]
    #23200954 - 05/08/16 01:39 AM (8 years, 8 months ago)

Yeah, I think pouring would be the way to go in this case.
It would save time and reduce your chances of contaminating the whole LC.
You don't want to have the lid off for too long, it doesn't matter how good your sterile techniques are, LC's are highly prone to contam given the high sugar content.

Look into LI's next time, they're a lot easier to work with and are very reliable.

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Re: if you make lc with un modified lid question. [Re: Psilosoulful]
    #23200991 - 05/08/16 02:06 AM (8 years, 8 months ago)

Lc are prone to contam thats why ive always done the same injection ports as my grain jars it just seems un modified lids are all the rage right now and haveing to replace silicone after each cycle sucks

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InvisibleSupalemonhaze
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Re: if you make lc with un modified lid question. [Re: tump]
    #23201001 - 05/08/16 02:16 AM (8 years, 8 months ago)

You should buy the rubber ports if you want them to last a bit longer. For my lc's I like leaving the hole empty and using tyvek. When it's time to inoculate I remove the ring, peel back the tyvek, fold it so the underside doesn't get dirty and use the hole instead of silicone. Doesn't matter how new the silicone is, my needles always tear small pieces of it and they end up in my LC usually contaminating it. With a hole you can use it multiple times without contaminating if you are careful.

Nowadays I also prefer to make multiple small batches of LC rather than 1 large batch. I use around 200ml so I won't have to keep using the same LC over and over. Much safer.

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Offlinecamplo
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Re: if you make lc with un modified lid question. [Re: tump]
    #23201056 - 05/08/16 02:43 AM (8 years, 8 months ago)

Quote:

tump said:
How do you clean the outside of syringe to not be a contam risk when sucking up the lc to inject the other jars of grains in glovebox




Sterilize the whole thing in tin foil wrap or in a jar. Sterilized, nutritious, media is prone to contam if not kept sterile, period.


--------------------
:three:

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InvisibleSupalemonhaze
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Re: if you make lc with un modified lid question. [Re: camplo]
    #23201080 - 05/08/16 02:57 AM (8 years, 8 months ago)

If that is supposed to bring him peace of mind, it won't. He intended to reach with his syringe inside his jar but you would still have to touch the syringe with your hand to pull the plunger. Best to either not use a syringe or use a hole so only the flame sterilized needle can go inside the jar.

I don't pc my syringes anymore, those with a rubber plunger will crack from the heat and the ones with a plastic plunger will make the outside plasic expand due to the plunger being such a tight fit. I prepare my syringes with boiling water, like that tek for spore syringes.

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Re: if you make lc with un modified lid question. [Re: Supalemonhaze]
    #23201135 - 05/08/16 03:34 AM (8 years, 8 months ago)

Yes i see now im just going to use the good old method to make sure its clean then just spay it in grain jar s

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