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OfflineJamur
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Registered: 05/02/16
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Stroma, what possible cause
    #23187231 - 05/04/16 01:11 AM (8 years, 8 months ago)


those two starter are inoculated from a same parent, but on several days after inoculation the one in the bottle got stroma but the one on petri dish didnt. what the possible cause of this anomaly?
(we inoculated A.bisporus in PDA, the picture taken on the 14th days after inoculation).
anyone could help? :smile:

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InvisibleInocuole
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Re: Stroma, what possible cause [Re: Jamur]
    #23187275 - 05/04/16 01:42 AM (8 years, 8 months ago)

Probably a bacteria or mold contending with the mycelium in those areas.  Typically won't happen on its own with a clean culture.

edit: didn't notice this was bisporus, maybe it does do that on its own, I wouldn't know since this is the forum for psychoactive mushrooms.


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OfflineJamur
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Re: Stroma, what possible cause [Re: Inocuole]
    #23187288 - 05/04/16 01:47 AM (8 years, 8 months ago)

so is it very likely caused by contaminant? because all culture in bottles have the same condition but not happened in the petri dish (we have about 18 bottles and 4 petridish).
edit : sorry , i am new in shroomery, whould you show me where i actually should post this kind of question?

Edited by Jamur (05/04/16 01:51 AM)

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Offlinemushpunx
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Re: Stroma, what possible cause [Re: Jamur]
    #23187326 - 05/04/16 02:09 AM (8 years, 8 months ago)

It still could have gotten contaminated by a lapse in sterile technique.

Curious about the bottles. Ive seen photos of people using bottles for agar work on some  asian farms, only they used a plug of polyfill to seal up the bottle/for gas exhange. What is covering the mouth of the bottle? It looks like a magazine page?

Are you working in a still air box or in front of a flow hood?

Gourmet and medicinal is the proper forum


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OfflineJamur
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Re: Stroma, what possible cause [Re: mushpunx]
    #23194605 - 05/06/16 06:21 AM (8 years, 8 months ago)

i cover the bottle mouth with cotton and some kind of magazine paper (tied by elastic/rubber band). i'm working in a front of a flow hood.

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InvisibleInocuole
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Re: Stroma, what possible cause [Re: Jamur]
    #23194925 - 05/06/16 09:21 AM (8 years, 8 months ago)

Actual cotton?  Not polyfill but cotton?  What.. country is this happening in?


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OfflineJamur
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Re: Stroma, what possible cause [Re: Inocuole]
    #23196747 - 05/06/16 06:52 PM (8 years, 8 months ago)

sorry, i mean i use a kind of polyfill, not natural cotton. it is in Indonesia

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