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OfflineTHK107
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Help with picking an agar section
    #23193084 - 05/05/16 05:34 PM (8 years, 8 months ago)

So I just looked at my agar plates and most of them are cotton like with little rhizmorphic growth except for one or two, and one has great rhizmorphic growth.  I was wondering what part of these you would section off to eventually isolate and put into fruiting.  I circled the parts I think I should section off.


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OfflineGiBBz
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Re: Help with picking an agar section [Re: THK107]
    #23193096 - 05/05/16 05:37 PM (8 years, 8 months ago)

I think it may be most aggressive and fastest populating growth.

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Invisibledankington
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Re: Help with picking an agar section [Re: GiBBz]
    #23193118 - 05/05/16 05:44 PM (8 years, 8 months ago)

Your intuitions were pretty spot on. The first pic, I'd ignore the section towards the center, I feel that's asking for trouble. The other edges there look nice though. But I'm partial to the last picture. On the right side of the left rhizo growth, there is a tiny part growing out further than the rest. That's what I'd snag. I'd also pick some of the far left of the right growth, right by the number.

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OfflineTHK107
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Re: Help with picking an agar section [Re: dankington]
    #23194272 - 05/06/16 12:32 AM (8 years, 8 months ago)

Quote:

dankington said:
Your intuitions were pretty spot on. The first pic, I'd ignore the section towards the center, I feel that's asking for trouble. The other edges there look nice though. But I'm partial to the last picture. On the right side of the left rhizo growth, there is a tiny part growing out further than the rest. That's what I'd snag. I'd also pick some of the far left of the right growth, right by the number.




I took a better picture of the more favorable one.  The other one had glad wrap blocking it, would you go for the red that has spread to the edge of the plate or the green still?


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Invisibledankington
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Re: Help with picking an agar section [Re: THK107]
    #23194600 - 05/06/16 06:18 AM (8 years, 8 months ago)

I still think the little part you have circled in green looks a bit stronger than the mycelium surrounding it. But by all means, if you have the extra plates, try some samples from the red section.

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