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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Storing sterilized grain until able to inoculate [Re: bodhisatta]
    #23198982 - 05/07/16 01:51 PM (8 years, 8 months ago)

Quote:

bodhisatta said:
They really should be ran at least a half hour to an hour before use too.




Not a requirement, by far. Plenty of folks use it without running it before hand. I do it myself with success. The success is due to the same reason a carpet will not kill your success rate, the flow will be sterile whatever the room's conditions. It's good practice to not have a carpet and to run it beforehand just like it's good practice to wash your hands and tubs before spawning, but very similiarly, not a requirement.

RR's advice going back a decade hasn't failed me yet so I don't mind being a tard. I'll be a successful tard at least.

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InvisibleBoogieman47
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Registered: 03/05/16
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Re: Storing sterilized grain until able to inoculate [Re: Supalemonhaze]
    #23199917 - 05/07/16 06:57 PM (8 years, 8 months ago)

The flow hood actually blows away any particles out of you're work area if you burn an incense on top of the flow it blows it up and away and mold and dust spores are way lighter then smoke so I think one is fine anywhere in NY sab I was getting jars full of mold over and over


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Invisibleblindingleaf
blue collar underworld
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Registered: 07/19/13
Posts: 22,008
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Re: Storing sterilized grain until able to inoculate [Re: Boogieman47]
    #23199961 - 05/07/16 07:08 PM (8 years, 8 months ago)

There is old ass carpet in the room where my hood is.  I cleaned it when I moved in.  I leave the window open, even when the hood is on...Its fine but I agree not ideal.  When I see a contam increase I'll stop.  Dog not allowed in there tho..fuck that.

Make ur fpm higher than 100...stronger flow...weak flow is cool if u in still air super clean room...but other than that let that baby blow


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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OfflineLuminous7


Registered: 01/26/16
Posts: 1,443
Last seen: 4 days, 22 hours
Re: Storing sterilized grain until able to inoculate [Re: blindingleaf]
    #23200364 - 05/07/16 09:26 PM (8 years, 8 months ago)

The SAB seems to be working fine for me. Even though i seem to do a really slopy transfer each time.
I transfered to all the jars of grain that had been sitting for a week , and there already starting to take off and look good.

My room is pretty dank as well. old carpet, pets ect.

SAB hasnt failed me yet. I used to use gloves attached but did the recent transfers with no gloves/sleeves attached . Still no problems


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InvisibleBoogieman47
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Re: Storing sterilized grain until able to inoculate [Re: Luminous7]
    #23200514 - 05/07/16 10:22 PM (8 years, 8 months ago)

That's good some people have great luck but literally everything I did got mold I was going to make a sab kinda like a glass beader once I get enough money but since I built my hood I've had no problems


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InvisibleSupalemonhaze
Spore syringe hater.
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Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Storing sterilized grain until able to inoculate [Re: blindingleaf]
    #23200813 - 05/08/16 12:25 AM (8 years, 8 months ago)

Quote:

blindingleaf said:
There is old ass carpet in the room where my hood is.  I cleaned it when I moved in.  I leave the window open, even when the hood is on...Its fine but I agree not ideal.  When I see a contam increase I'll stop.  Dog not allowed in there tho..fuck that.

Make ur fpm higher than 100...stronger flow...weak flow is cool if u in still air super clean room...but other than that let that baby blow




Mine is actually somewhere around 200fpm. It's said that the closer you are to the rated flow, the more effiecient it will be in cleaning the air. But I agree, nothing can move against a flow that strong.

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Invisibleblindingleaf
blue collar underworld
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Re: Storing sterilized grain until able to inoculate [Re: Supalemonhaze]
    #23201213 - 05/08/16 05:09 AM (8 years, 8 months ago)

to be fair, its on the second floor.  i probably wouldn't keep window cracked if it was first floor.  but in the summer, the window AC unit runs perpendicular to the flow hood in the same room and I'm fine.

I think SAB's are awesome.  i had 100% success with agar>agar and agar>masters.  it was g2g where my success would fall.  but i was using a 66qt tote….stupid mistake.

IMO, the more time u spend growing, the more appropriate a FH becomes.

it doesn't guarantee success,but IME, certainly improves it.

if ur living the good life with game consoles, big TV's, have a nice car etc, then yea, the purchase seems too much for a hobby u don't spend much time with.

if ur just some dude who likes to grow, spends a good amount of free time doing it, and has little else to spend money on (except good beer and good weed), then i think its worth it.

a 12x24" is about same size as a 110qt SAB if u build it right.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Storing sterilized grain until able to inoculate [Re: blindingleaf]
    #23201239 - 05/08/16 05:47 AM (8 years, 8 months ago)

Yeah, I'm on the 2nd story as well but I don't open the windows here. I reckon the stale air inside is fresher than the air outside where I live atm :rofl:.

I agree, a hood will not magically keep your work from contaminating. I normally discourage new folks from getting them for a number of reasons, the most common would be that their sterile tek is usually the fault (even though a hood is easier to use IMO) but more importanly, a newbie might be crazy about mycology for the first couple of months but gets tired of it real quickly. One needs to be real serious about it before he starts spending that amount of money and he needs to be sure that if there are any problems, they aren't from his sterile tek.

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OfflineGreen Bastard
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Re: Storing sterilized grain until able to inoculate [Re: Supalemonhaze]
    #23204480 - 05/09/16 12:23 AM (8 years, 8 months ago)

Yer all probably gonna be hating on me for saying this (lol!)...

Took a 5 month hiatus, this winter, from growing. 3 weeks ago, I pressure cooked 70 jars of rye @ 22 psi, for 100 minutes. Innoclated them from 6 month old masters, that sat in a cool room, all that time. When shaken, and opened in front of my flow hood, they smelled fresh and sweet. The 70 jars all colonized fast. No contams. Just spawned 50 of them, to monotubs, 3 days ago. They're already over 50% colonized, and very healthy looking.

And. I had 15 quarts of rye, PCed back in November, not inoculated, sitting in the same room. They smelled nice and tasty. Nocked them up with some masters, made last September. Colonizing just fine. If all of your teks are on point, you'd be amazed at what you can get away with!!

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InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Storing sterilized grain until able to inoculate [Re: Green Bastard]
    #23204505 - 05/09/16 12:34 AM (8 years, 8 months ago)

It would heavily depend on the endospore count in your grains and how long you PC for. I have used 2 week old jars successfully but I almost always use them fresh just in case.

A grain jar is never really sterile, PCing just opens a window for our mushroom mycelium to grow before the endospores recover.

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OfflineLuminous7


Registered: 01/26/16
Posts: 1,443
Last seen: 4 days, 22 hours
Re: Storing sterilized grain until able to inoculate [Re: Supalemonhaze]
    #23205352 - 05/09/16 10:23 AM (8 years, 8 months ago)

mine are doing great even tho they sat for a week un inoculated. I'm not to worried.


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OfflineLuminous7


Registered: 01/26/16
Posts: 1,443
Last seen: 4 days, 22 hours
Re: Storing sterilized grain until able to inoculate [Re: Green Bastard]
    #23205360 - 05/09/16 10:27 AM (8 years, 8 months ago)

Quote:

Green Bastard said:
Yer all probably gonna be hating on me for saying this (lol!)...

Took a 5 month hiatus, this winter, from growing. 3 weeks ago, I pressure cooked 70 jars of rye @ 22 psi, for 100 minutes. Innoclated them from 6 month old masters, that sat in a cool room, all that time. When shaken, and opened in front of my flow hood, they smelled fresh and sweet. The 70 jars all colonized fast. No contams. Just spawned 50 of them, to monotubs, 3 days ago. They're already over 50% colonized, and very healthy looking.

And. I had 15 quarts of rye, PCed back in November, not inoculated, sitting in the same room. They smelled nice and tasty. Nocked them up with some masters, made last September. Colonizing just fine. If all of your teks are on point, you'd be amazed at what you can get away with!!



Damn that's not bad!! good to know they will last so long with out any issues.


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Offlinespacechildo
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Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Re: Storing sterilized grain until able to inoculate [Re: Luminous7]
    #23205394 - 05/09/16 10:39 AM (8 years, 8 months ago)

try doing that consistently and then tell me there arent any issues..

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OfflineGreen Bastard
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Re: Storing sterilized grain until able to inoculate [Re: spacechildo]
    #23205865 - 05/09/16 12:23 PM (8 years, 8 months ago)

I'll keep ya all updated...

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