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Offlineregistdewer
Stranger
Registered: 05/05/16
Posts: 21
Last seen: 8 years, 3 months
Problems just happen, help needed.
    #23192573 - 05/05/16 02:45 PM (8 years, 8 months ago)

Hello to all members, this is my first post,

about my story:
I bought spore syringe from a local guy, I used it to inoculate 8 jars with 100% success. then the casing problem happened, the soil i used for casing was contaminated and destroyed all my first try, and I lost all of them,
The guy i bought syringe from had told me i can suck some more water into the syringe as it is so dense. I added distilled water (bought a gallon from drug store) and after that none of my jars were good. I get no contamination. 0% . but hardly mycelium appears.

If by any chance any mycelium spot appears, it will appear after 2 weeks. I saw pictures in the internet were many guys got mycelium spots only after 2 days, this problem happended after i suck water up into syringes.

Next time I used a mushroom cap to make a syringe. I put in in top of a 20ml cup full of same distilled water from same gallon. it look very dense. but success rate is 0%. again i get no contamination in the jars. but no mycelium too.

I don't know if the problem is water, or the mushroom caps dropped no spores. although the water looked near black.

The other main problem I have is that I used agar plate(10% sucess rate) and I put live tissue into jars. after some successful inoculation . after a few days when I mixed the jars to break down mycelium. then they grow no more. they are all dead. suddenly after mixing the jar they all died. i put them in same condition as they were to see if any contamination appears. nothing. no mycelium. no contam.

I got 2 prints from a supplier, can I use distilled injection water. the water used for body injection to make syringes? or boil a bottled water?

Please let me know if you have any idea.
Thanks,
Happy shrooming

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InvisibleSupalemonhaze
Spore syringe hater.
Male


Registered: 10/02/15
Posts: 6,725
Loc: 12" down Europe's butthole
Re: Problems just happen, help needed. [Re: registdewer]
    #23192584 - 05/05/16 02:50 PM (8 years, 8 months ago)

Jars that don't recover after a shake are usually contaminated with bacteria.

I am having a bit of trouble understanding your post, what happened with the agar plates that you inoculated? did they get a milky looking growth?

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Offlinetombosley8
Full on... Bossley Baggins
 User Gallery


Registered: 10/14/13
Posts: 3,660
Last seen: 6 months, 13 days
Re: Problems just happen, help needed. [Re: Supalemonhaze]
    #23193056 - 05/05/16 05:25 PM (8 years, 8 months ago)

what are youi innoculating,  grain?

are you using a sab?

how many cc's of spore solution are you using?

how are you preparing the spawn?


--------------------

Edited by tombosley8 (05/05/16 05:26 PM)

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