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kezza413
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Looking to start my first attempt at growth - Questions
#23187538 - 05/04/16 05:23 AM (8 years, 8 months ago) |
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Hey guys i picked two meanies on the weekend, out of season. And i'm thinking i might start a grow or attempt to. From all the guides i've looked at people are using pregained spores etc.
How would I recreate this process without using sterilized prints etc. I basically only have 2 dried blue meanies.
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Inocuole
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Re: Looking to start my first attempt at growth - Questions [Re: kezza413]
#23187593 - 05/04/16 06:06 AM (8 years, 8 months ago) |
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First you'll need impeccable sterile technique to be able to pull clean spores off a wild mushroom... The only possible way to salvage that is to start with agar and attempt a clone from inner tissue or have some really great luck with spores swabs.
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kezza413
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Re: Looking to start my first attempt at growth - Questions [Re: Inocuole]
#23187696 - 05/04/16 07:08 AM (8 years, 8 months ago) |
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So i drop potentially contam spores onto an agar?
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Josex
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Re: Looking to start my first attempt at growth - Questions [Re: kezza413]
#23187709 - 05/04/16 07:17 AM (8 years, 8 months ago) |
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This is one way to do it and if you do it right you may succeed.
-Get yourself a sterile swab and swipe it across the gills under the cap. -Dab the swab on the surface of the agar plate. -Research "sandwich agar tek" and do it.
Bacteria can't grow through agar but mycelium can, so if you perform correctly the agar sandwich tek you should see mycelium that's going to be clean of bacteria.
Once you transfer a wedge, keep making transfers to new agar plates until you're sure you have a clean culture.
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kezza413
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Re: Looking to start my first attempt at growth - Questions [Re: kezza413]
#23187713 - 05/04/16 07:19 AM (8 years, 8 months ago) |
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Also to double check steps im going to need to take.
Step 1. Agar Creation
Step 2. Drop in spores
Step 3. Agar > BRF Cakes? (or do i need to agar > agar again?)
Step 4. BRF cakes > FC
Step 5. ???
Step 6. Success
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kezza413
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Re: Looking to start my first attempt at growth - Questions [Re: kezza413]
#23187718 - 05/04/16 07:21 AM (8 years, 8 months ago) |
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Edited because i read some more
Ok cool, last question does it have to be antibiotic agar? And where do i buy this stuff
Edited by kezza413 (05/04/16 07:30 AM)
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kezza413
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Re: Looking to start my first attempt at growth - Questions [Re: kezza413]
#23187722 - 05/04/16 07:23 AM (8 years, 8 months ago) |
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Also any recommended agar for a first timer?
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Josex
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Re: Looking to start my first attempt at growth - Questions [Re: kezza413]
#23187784 - 05/04/16 07:50 AM (8 years, 8 months ago) |
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Quote:
kezza413 said: Also any recommended agar for a first timer?
I'll be frank with you man, you want to attempt a moderately advanced technique for which you're not prepared at all.
It'd be a lot wiser to buy a spore print from a reputable vendor and be done with it.
This would be your best option if you want to work with agar.
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kezza413
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Re: Looking to start my first attempt at growth - Questions [Re: Josex]
#23187799 - 05/04/16 07:56 AM (8 years, 8 months ago) |
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I live in australia. Can't buy spores legally :/
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kezza413
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Re: Looking to start my first attempt at growth - Questions [Re: kezza413]
#23187808 - 05/04/16 08:00 AM (8 years, 8 months ago) |
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thanks for your help guys i think i understand.
Here we go!
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HEEBSHOUSE
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Re: Looking to start my first attempt at growth - Questions [Re: kezza413]
#23187811 - 05/04/16 08:01 AM (8 years, 8 months ago) |
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Austrailian customs control are pretty strict to. They check everything lol.
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Inocuole
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Re: Looking to start my first attempt at growth - Questions [Re: HEEBSHOUSE]
#23188692 - 05/04/16 01:27 PM (8 years, 8 months ago) |
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If it's an actual blue meanie it's not going to thank you for trying to grow it on BRF cakes either..
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jimmyjams
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Re: Looking to start my first attempt at growth - Questions [Re: Inocuole]
#23188934 - 05/04/16 02:59 PM (8 years, 8 months ago) |
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Spore prints come in a letter it's impossible for customs to scrutinize every incoming letter they'd come to halt. Trust me it's very doable.
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HEEBSHOUSE
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Re: Looking to start my first attempt at growth - Questions [Re: jimmyjams]
#23189056 - 05/04/16 03:36 PM (8 years, 8 months ago) |
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Very true . Just I know Aus and Canada customs are pretty good at their jobs. Ive had a few seizure letters myself back in the old days for little packages i was sure would make it threw.
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kezza413
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Re: Looking to start my first attempt at growth - Questions [Re: HEEBSHOUSE]
#23189364 - 05/04/16 05:01 PM (8 years, 8 months ago) |
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I've read some horror stories about Australian customs and when i got a sprouting field nearby i would rather just put in the hard work to get it done.
I am not going to give up if my first try doesn't work. I am very much so dedicated to this adventure.
And yes they are blue meanies... what do you recommend then for blue meanies? Grain?
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kezza413
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Re: Looking to start my first attempt at growth - Questions [Re: kezza413]
#23189414 - 05/04/16 05:17 PM (8 years, 8 months ago) |
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Sorry about the million questions guys xD.
I am looking through as much information as i can find, but there is so many methods, and so many steps that people do differently. So while one guide gives you flawless information, it has a flaw for example BRF cakes. I can't find any other suggestions specifically for blue meanies.
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