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Munchauzen
Registered: 06/22/11
Posts: 14,343
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Quote:
pierre2008 said: Post deleted by pierre2008<p>Reason for deletion: not interested in the thread
lol
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Fungeyes
Stranger
Registered: 08/26/17
Posts: 136
Last seen: 2 years, 7 months
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
#24596234 - 09/01/17 02:50 PM (6 years, 6 months ago) |
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Can't wait to try this out
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miketheexplorer
mushfriend
Registered: 08/15/10
Posts: 500
Last seen: 5 years, 3 months
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Re: Agar as easy as 1-2-3! [Re: Fungeyes] 1
#24596806 - 09/01/17 07:11 PM (6 years, 6 months ago) |
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Munch, Thank you for you tutorial videos I learn a lot from them.
Edited by miketheexplorer (09/01/17 07:25 PM)
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senseit
Listening
Registered: 01/16/16
Posts: 156
Last seen: 6 months, 29 days
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Quote:
pierre2008 said: They way we do and say things is everything in terms of results, personal and professional.
Leaving it ... Got 100 petri dishes ordered Enjoy the ride 1 cent :-)
You'll still need to know how to run your pressure cooker properly with petri dishes. I was concerned by your question that you would run the cooker with too little water. According to what I've read, running a pressure cooker dry weakens it and is possibly dangerous. I learned a lot from watching Bod's Easy AF how to use your pressure cooker video, like how to keep my jars from getting mineral buildup and how to run the pressure cooker with less noise. Even if you know how to run the pressure cooker, you still might learn stuff you didn't know.
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apollo321
MycoNoob
Registered: 05/17/16
Posts: 13
Loc: CA
Last seen: 4 years, 1 month
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Re: Agar as easy as 1-2-3! [Re: hamloaf]
#24598464 - 09/02/17 01:15 PM (6 years, 6 months ago) |
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Love this clear, simple recipe and the video that goes with it. Thanks! The flakes are so much nicer to work with than LME.
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miketheexplorer
mushfriend
Registered: 08/15/10
Posts: 500
Last seen: 5 years, 3 months
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Re: Agar as easy as 1-2-3! [Re: apollo321]
#24599274 - 09/02/17 08:37 PM (6 years, 6 months ago) |
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Quote:
senseit said:
Quote:
pierre2008 said: They way we do and say things is everything in terms of results, personal and professional.
Leaving it ... Got 100 petri dishes ordered Enjoy the ride 1 cent :-)
You'll still need to know how to run your pressure cooker properly with petri dishes. I was concerned by your question that you would run the cooker with too little water. According to what I've read, running a pressure cooker dry weakens it and is possibly dangerous. I learned a lot from watching Bod's Easy AF how to use your pressure cooker video, like how to keep my jars from getting mineral buildup and how to run the pressure cooker with less noise. Even if you know how to run the pressure cooker, you still might learn stuff you didn't know.
Agree, been doing research on agar for several weeks, seen Bods vids several times, there's also many good videos on youtube Got the petri dishes today Bought the pressure cooker today, clear operating instructions Made the still box Made the Malt agar ... like the color red for good contrast Sometimes you just have to say fuck it and do it Easy and quite fun ... think i'll be doing this a lot more lol cheers mate!
-------------------- Gratitude increases the value of what we already have
Edited by miketheexplorer (09/02/17 08:43 PM)
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Munchauzen
Registered: 06/22/11
Posts: 14,343
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another piece of advice, remove the lid from your still air box. just wipe off your surface there and work on that. its a lot easier to work on a stable surface than a plastic lid.
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miketheexplorer
mushfriend
Registered: 08/15/10
Posts: 500
Last seen: 5 years, 3 months
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
#24599399 - 09/02/17 10:08 PM (6 years, 6 months ago) |
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Munch, you literally read my mind! I was wondering if I could remove the lid from the SAB, the plates were sliding all over the place
Thank you!!! Really appreciate it
-------------------- Gratitude increases the value of what we already have
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Knockoutartist
Night Night
Registered: 08/09/17
Posts: 66
Last seen: 3 years, 28 days
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miketheexplorer
mushfriend
Registered: 08/15/10
Posts: 500
Last seen: 5 years, 3 months
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
#24599565 - 09/03/17 12:29 AM (6 years, 6 months ago) |
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Quote:
Munchauzen said: another piece of advice, remove the lid from your still air box. just wipe off your surface there and work on that. its a lot easier to work on a stable surface than a plastic lid.
Munch, is just saw your video on working with a SAB. I think you spray soapy water or ISO 70, then cover the SAB.
I just saw this, of a guy putting all his stuff inside the SAB and then spraying with disinfectant ... does this work well?
-------------------- Gratitude increases the value of what we already have
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Munchauzen
Registered: 06/22/11
Posts: 14,343
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its soapy water. never spray isopropyl alcohol in a sab. it effectively creates a bomb.
and thats a big fuck no to spraying disinfectant in your sab. still air and a peaceful soul is all you need to do sterile agar work.
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miketheexplorer
mushfriend
Registered: 08/15/10
Posts: 500
Last seen: 5 years, 3 months
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
#24599742 - 09/03/17 03:06 AM (6 years, 6 months ago) |
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-------------------- Gratitude increases the value of what we already have
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bodhisatta
Smurf real estate agent
Registered: 04/30/13
Posts: 61,891
Loc: Milky way
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Quote:
pierre2008 said:
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bill bixby
Stranger
Registered: 06/09/17
Posts: 36
Last seen: 4 years, 6 months
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Re: Agar as easy as 1-2-3! [Re: bodhisatta]
#24604470 - 09/04/17 10:20 PM (6 years, 6 months ago) |
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tried this the other day and everything looking good so far. very easy to remember cheers munch
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Munchauzen
Registered: 06/22/11
Posts: 14,343
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Re: Agar as easy as 1-2-3! [Re: bill bixby]
#24604512 - 09/04/17 10:37 PM (6 years, 6 months ago) |
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BreakTheSOB
Stranger
Registered: 07/09/17
Posts: 162
Last seen: 6 years, 4 months
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
#24605368 - 09/05/17 10:23 AM (6 years, 6 months ago) |
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i use your softer recipe alot man, thanks
-------------------- "A common sense interpretation of the facts suggests that a super intellect has monkeyed with physics, as well as with chemistry and biology, and that there are no blind forces worth speaking about in nature." Physicist Fred Hoyle
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jeezy117
FunGuy
Registered: 05/31/09
Posts: 100
Last seen: 5 years, 10 months
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Quote:
pierre2008 said:
Quote:
senseit said:
Quote:
pierre2008 said: They way we do and say things is everything in terms of results, personal and professional.
Leaving it ... Got 100 petri dishes ordered Enjoy the ride 1 cent :-)
You'll still need to know how to run your pressure cooker properly with petri dishes. I was concerned by your question that you would run the cooker with too little water. According to what I've read, running a pressure cooker dry weakens it and is possibly dangerous. I learned a lot from watching Bod's Easy AF how to use your pressure cooker video, like how to keep my jars from getting mineral buildup and how to run the pressure cooker with less noise. Even if you know how to run the pressure cooker, you still might learn stuff you didn't know.
Agree, been doing research on agar for several weeks, seen Bods vids several times, there's also many good videos on youtube Got the petri dishes today Bought the pressure cooker today, clear operating instructions Made the still box Made the Malt agar ... like the color red for good contrast Sometimes you just have to say fuck it and do it Easy and quite fun ... think i'll be doing this a lot more lol cheers mate!
Hey Munch,
I've also been trying to step up to the world of agar and have watched the videos and built my now 3rd SAB after not taking the time to properly choose one that is the right size THE FIRST TIME.
Since I forgot to make spore prints the last few grows I did I had to buy another couple spore syringes. Considering that this wont be a tiger drop, would it be wise to drop the powdered agar quantity just a tad?
I also picked up an inoculation loop set. Is this even worth the hassle this round since I'm coming from a syringe?
I'm super stoked to try this. I'll be holed up the house this weekend for the hurricane and can't think of a better time to bite the agar bullet. I have finally overcame the first couple grow jitters and want to make sure I do everything the right way this time.
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Munchauzen
Registered: 06/22/11
Posts: 14,343
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Re: Agar as easy as 1-2-3! [Re: jeezy117]
#24609609 - 09/06/17 06:41 PM (6 years, 6 months ago) |
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no need to change the recipe. it works great for spores and transfers alike.
no need for a loop with the syringe. just heat up the dispensing needle to red hot, then make a little slice thru the middle of the agar to make a little valley and drop your solution into that.
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Cujllickduo
Registered: 06/13/15
Posts: 19,552
Loc: England
Last seen: 3 years, 8 months
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
#24615889 - 09/09/17 11:58 AM (6 years, 6 months ago) |
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first time with agar, ive 15 plates and in 10minutes it'll have been 30mins after they were set do i then place in pressure cooker for 30mins and leave out on the side for next day spore transfer ?
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Munchauzen
Registered: 06/22/11
Posts: 14,343
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yes you need to pressure cook them for 30 minutes. after that, they are good to use for quite some time.
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