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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
#24319440 - 05/14/17 06:28 PM (7 years, 1 month ago) |
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PreparationH
apply daily

Registered: 03/28/05
Posts: 18,361
Loc: Amsterdam
Last seen: 1 hour, 57 minutes
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
#24532421 - 08/05/17 12:48 PM (6 years, 10 months ago) |
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posting in here so I can find it easily!
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miketheexplorer
mushfriend



Registered: 08/15/10
Posts: 500
Last seen: 5 years, 6 months
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Re: Agar as easy as 1-2-3! *DELETED* [Re: PreparationH]
#24594918 - 08/31/17 11:54 PM (6 years, 9 months ago) |
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Enjoy
-------------------- Gratitude increases the value of what we already have
Edited by miketheexplorer (09/01/17 10:34 AM)
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senseit
Listening


Registered: 01/16/16
Posts: 156
Last seen: 9 months, 27 days
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Quote:
pierre2008 said: I'm diving in and trying out I'm gonna give Pastywhyte's Easy Agar!!
I have 2 questions, I'm stuck on: 1. Do I need to drill the hole in the Glad minis or do I cover, wrap in foil and put in PC?
2. How much water should I put into the pressure cooker? The minis are very small
Thank you
1. Yes they will be crushed otherwise 2. You should put the minimum amount that your pressure cooker requires as stated in the manual. I put two layers of mason jar lids in the bottom and then the trivet/rack that came with the pressure. I fill the water to the trivet which is a few inches of water. The plates go on top of that.
Those questions make it seem like you haven't read the pasty plate thread closely or are ignoring what it says for some reason. You might want to read it again.
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miketheexplorer
mushfriend



Registered: 08/15/10
Posts: 500
Last seen: 5 years, 6 months
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Re: Agar as easy as 1-2-3! *DELETED* [Re: senseit]
#24594987 - 09/01/17 01:34 AM (6 years, 9 months ago) |
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Post deleted by pierre2008Reason for deletion: not interested in the thread
-------------------- Gratitude increases the value of what we already have
Edited by miketheexplorer (09/01/17 01:56 AM)
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BreakTheSOB
Stranger



Registered: 07/09/17
Posts: 162
Last seen: 6 years, 7 months
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I drill well actually melt mine in the side with a soldering iron the cover with mp tape. I'm using 2 oz plastic shot cups from dollar tree with lids. Do around 27 a pop in a 23 qt presto. I have a lot of agar going, I mean alot, just started 7 weeks ago on t8 now
-------------------- "A common sense interpretation of the facts suggests that a super intellect has monkeyed with physics, as well as with chemistry and biology, and that there are no blind forces worth speaking about in nature." Physicist Fred Hoyle
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,885
Loc: Canada
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Quote:
pierre2008 said:
Quote:
senseit said:
Quote:
pierre2008 said: I'm diving in and trying out I'm gonna give Pastywhyte's Easy Agar!!
I have 2 questions, I'm stuck on: 1. Do I need to drill the hole in the Glad minis or do I cover, wrap in foil and put in PC?
2. How much water should I put into the pressure cooker? The minis are very small
Thank you
1. Yes they will be crushed otherwise 2. You should put the minimum amount that your pressure cooker requires as stated in the manual. I put two layers of mason jar lids in the bottom and then the trivet/rack that came with the pressure. I fill the water to the trivet which is a few inches of water. The plates go on top of that.
Those questions make it seem like you haven't read the pasty plate thread closely or are ignoring what it says for some reason. You might want to read it again.
Thank you appreciate the information in your response.
Don't appreciate your ignorant comment at the end. You've been here a little over a year, and I've been here over 7 ... if you can't say anything kind, censor yourself for the sake of everyone's sanity
I don't see his comment as ignorant. Just an honest opinion and it was good of him to mention it. Criticism is not easy to recieve but it's critical to success in life. This hobby can be challenging and if you can't handle some frank comments it's going to be even harder when you get struck with some real challenges. You are growing a felony, imagine how sensitive the authorities will be if you get caught.
Length of tenure has very little to do with skill set. You have been here longer than I have. What does that say?
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muchlove
Duality



Registered: 05/02/17
Posts: 89
Last seen: 3 years, 7 months
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Re: Agar as easy as 1-2-3! [Re: Munchauzen]
#24595363 - 09/01/17 08:08 AM (6 years, 9 months ago) |
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dhype773
Enter the Void



Registered: 10/29/15
Posts: 2,182
Loc: Valhalla
Last seen: 6 years, 7 months
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Re: Agar as easy as 1-2-3! [Re: muchlove]
#24595400 - 09/01/17 08:26 AM (6 years, 9 months ago) |
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Am I the only one who finds it completely impossible to make PastyPlates with no condensation? I've made a few batches now, and I don't get what I'm doing wrong. I'm about to make 16 plates tonight, and would like to get them out of the pc with no water on the sides. I always let the pc cool way longer than 8 hours. Usually closer to 20/24 hours by the time I'm able to take everything out. Advice?
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lovelaughlibs
Dopamine Slave



Registered: 10/14/15
Posts: 1,811
Loc: England
Last seen: 6 years, 4 months
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Re: Agar as easy as 1-2-3! [Re: dhype773]
#24595407 - 09/01/17 08:33 AM (6 years, 9 months ago) |
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Same here, visibility is kinda impaired, it's largely why I did some pour petris.
-------------------- Ask and ye shall receive; Seek and ye shall find.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,885
Loc: Canada
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Condensation is pretty much a non issue for me. Usually dries up in a few days. It's not a vector. Make your plates in advance.
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dhype773
Enter the Void



Registered: 10/29/15
Posts: 2,182
Loc: Valhalla
Last seen: 6 years, 7 months
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Re: Agar as easy as 1-2-3! [Re: Pastywhyte]
#24595452 - 09/01/17 08:54 AM (6 years, 9 months ago) |
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I do, I have them in the fridge, but it never clears up, even after a month. But I guess that's because I leave the foil on until I use them
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,885
Loc: Canada
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Re: Agar as easy as 1-2-3! [Re: dhype773]
#24595492 - 09/01/17 09:12 AM (6 years, 9 months ago) |
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Take the foil off and it clears fast. I leave it on until I use them and they clear in a few days, usually in time to observe the growth. Also never store unused plates in the fridge, if there are CFU'S present you want them to germ and grow so you know not to use them.
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dhype773
Enter the Void



Registered: 10/29/15
Posts: 2,182
Loc: Valhalla
Last seen: 6 years, 7 months
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Re: Agar as easy as 1-2-3! [Re: Pastywhyte]
#24595542 - 09/01/17 09:36 AM (6 years, 9 months ago) |
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Thanks man!
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miketheexplorer
mushfriend



Registered: 08/15/10
Posts: 500
Last seen: 5 years, 6 months
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Re: Agar as easy as 1-2-3! [Re: Pastywhyte]
#24595657 - 09/01/17 10:33 AM (6 years, 9 months ago) |
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I am not discussing tenure as it relates to skill set, so not sure where you got that from. An encouraging attitude is everything in sharing knowledge, especially in an forum where everyone is trying to improve.
-------------------- Gratitude increases the value of what we already have
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,885
Loc: Canada
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Tenure came in when you compared your reg date to his. As for encouragement I feel that people demand too much. It wasn't like he called you a fucking tard and a waste of skin. He just said that it felt like you weren't absorbing as much as you should have and that you should reread. The fact that he took the time to try and help you improve should be enough. Nothing to be butthurt over IMO.
I don't sugar coat my advice, I simply don't get paid enough to do so. Take what I give you or leave it, it's your grow not mine.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Agar as easy as 1-2-3! [Re: Pastywhyte]
#24595802 - 09/01/17 11:23 AM (6 years, 9 months ago) |
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I do the same. Take the foil off. Let em sit a few days and the condensation is gone.
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miketheexplorer
mushfriend



Registered: 08/15/10
Posts: 500
Last seen: 5 years, 6 months
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Re: Agar as easy as 1-2-3! [Re: Pastywhyte]
#24595826 - 09/01/17 11:33 AM (6 years, 9 months ago) |
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They way we do and say things is everything in terms of results, personal and professional.
Leaving it ... Got 100 petri dishes ordered Enjoy the ride 1 cent :-)
-------------------- Gratitude increases the value of what we already have
Edited by miketheexplorer (09/01/17 11:40 AM)
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,885
Loc: Canada
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So the only way you are willing to accept advice or criticism is if the person giving you the help is simultaneously kissing your ass? Seems to me that it should be the other way around.
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hamloaf
Pork Block ®©™√



Registered: 12/23/09
Posts: 22,046
Loc: ation undisclosed.
Last seen: 2 hours, 25 minutes
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Re: Agar as easy as 1-2-3! [Re: eatyualive]
#24596036 - 09/01/17 01:07 PM (6 years, 9 months ago) |
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Quote:
eatyualive said: I do the same. Take the foil off. Let em sit a few days and the condensation is gone.
Environmental conditions come into play when it comes to the condensation of all media vessels. If your temps swing 10-15 degrees, you will see condensation. The best fix for condensation is to keep your spawning area at a constant temperature and relative humidity. I go with 70-72F & around 50% RH.
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